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To screen the sensitive and resistant yeast mutant when they treated with drug, the recombine technology of yeast gene should be applied to obtain the clone expressing the homeotic gene.

通过使用药物对特定的酵母基因突变株进行干预，筛选出对药物敏感或抵抗的突变株，然后利用酵母基因重组技术，使突变株再表达同源的基因，从而获得再表达同一基因的克隆。

Objective To investigate the mutation characteristics of paired box homeotic gene 9(PAX9) and mus-cle segment homeobox gene 1(MSX1) of patients with congenital oligodontia.

目的 探讨多数牙先天缺失患者的人类成对盒基因(PAX9)和肌节同源盒基因(MSX1)突变位点，为该疾病的病因学研究提供依据。

Because homeotic gene mutation and floral organ variation is a direct relationship,therefore the mutant application is the current functional genomics study developmentdirection. We discovered one mutant ah from line 458, which was a line ofthe twin-seedling strain W2555.ln this study, we have investigated the morphogenesisprogress, genetic analysis of mutant traits and molecular tagging of related gene.

目前，由于在水稻中发现的花器官突变体很少，所以对水稻花发育的研究主要是利用双子叶植物MAS-box基因的保守序列设计引物，在水稻基因组或者cDNA文库中筛选与双子叶植物花器官发育相对应的MADS基因，因为同源异型基因的突变与花器官的变异是直接对应的关系，所以突变体的应用是当前功能基因组学的发展方向。

The term"homeodomain"has evolved to define a class of protein domains that has recognizable similarity to a 60 amino acid motif (encoded by 180 bp homeobox sequences) originally recognized in three Drosophila homeotic and segmentation proteins. The homeodomains that have been tested contain sequence-specific DNA binding activities and are part of larger proteins that function as transcriptional regulators.

其中一类转录因子通过同源域结合特定的DNA序列，这种特殊的转录因子都包含一个同源盒，它有180bp序列可以编码DNA结合同源域，称同源盒基因（Homeobox gene，Hox gene），带有同源框的基因经过转录产生带有同源域的蛋白质。

Inaddition to that,LFY has a second role in the activation of the floral homeotic genes that specify the identity of organs in the flower and is a key intergrator of the outputs of floral inductive pathways.DFL, a FLORICAULA/LEAFY homologous gene,which cloned from C.lavandulifolium,was a key gene of florescence for C.lavandulifolium.

LEAFY基因在植物成花过程中意义重大，它不仅决定着花由营养生长向生殖生长的转变，它还接连GA成花途径和长日途径的信号继而诱导花分生组织的转变，同时还是活化花器官基因的关键基因。

The sequence of amino acid of clone CR271 was highly homologic (61% identify and 76% similarity) to blight resistance gene Xal of rice to Xanthomonas oryzae pv. Oryzae. All other fragments showed significantly amino acidhomology to the NBS-LRR type resistance gene that have been cloned. The length of JR2 and JR3 fragments were 1321bp and 896bp respectively, which did not showed significantly homology on the level of amino acid and nucleotide acid to the sequences that have been known.

其中CR271与水稻白叶枯病抗病基因Xa1(AB002266)氨基酸同源性有61％相同，76％相似，其它片段与已克隆的NBS-LRR类抗病基因同源片段都具有较高的同源性；所获得的右翼序列JR2DNA片段长为1321bp，JR3DNA片段长为896bp，此两片段在核苷酸水平及氨基酸水平上均未找到有显著同源的序列。

Comparative analysis of some rbcL gene cDNA sequences between wheat and other species were also carried out, the result suggested that the rbcL gene was highly conserved in examined species, common wheat is more closer related with Hordeum, Psathyrostachys, Leymus, Elymus than with Oryza and Zea.

进一步比较分析了在相关物种中rbcL基因的cDNA序列，结果显示，rbcL基因的编码区在不同属的物种间高度保守，并从分子水平表明小麦与大麦、新麦草、赖草、披碱草属等的亲源关系比与水稻、玉米等的近。

The functional target of the microRNA appears to be the HOXD10 gene, one of the Hox gene family involved in directing embryo development.

微RNA的功能目标似乎是HoxD10基因，它是参与引导胚胎发育的Hox基因家族的一个成员。

All of them included hAAT gene as the reporter gene.BALB/c mice were transfected with the plasmids by hydrodynamical transfection technology.

但该技术的缺点是转染基因多瞬时表达，外源基因在体内高效表达的时间通常不超过1周，限制了该技术的广泛应用。

Lucidum. Then we clone the gene, glfks1 and glfks2. Hydropathy analysis predicts that GlFks1 and GlFks2 are integral membrane protein with about 16 and 14 transmembrane helices.In genomic southern blot analysis, glfks1 and glfks2 both are single copy gene.

cerevisiae相关之β-(1，3)-D-葡聚醣合成酶基因fks相似之序列，并将其选殖出来，分别得到glfks1与glfks2，利用hydropathy分析，显示GlFks1与GlFks2分别具有16个与14个transmembrane helix，且推测GlFks1与GlFks2应也是属於嵌入的membrane protein。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。