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The upstream regulatory region of the storage protein was separated from Glycine max variety "Ji Nong T7018" genomic DNA, according to published glycinin 11S G7 subunit (Gy7) gene(GenBank AF319776) sequence, a pair of primers was desgeined, in order to amplify the array before Gy7 gene initiation codon.

我们以大豆品种&吉农T7018&的基因组DNA为模板，根据已发表11S球蛋白G7亚基(Gy7)基因(GenBankAF319776)序列，设计一对引物，通过PCR扩增得到Gy7基因的启动子序列。

Graminaceae ; Tissue culture: Regulation of gene expression ; Micropropagation ; Gremplasm conservation ; Crop breeding ; Somatic hybrization ; Gene transfer techniques ; Plant genie engineering

禾本科植物；组织培养；基因表达的调控；无性系繁殖；种质保存；作物育种；体细胞杂交；基因转移技术；植物基因工程

Our result showed that P450scc expression was observed in hippocampus, habenular nucleus, neuron nucleus of thalamus and hypothalamus, and cerebral neocortex. The colocalization of P450scc and NeuN demonstrated that most of P450scc exist in neuron cell in these regions. In order to clarify the transcriptional regulation of CYP11A1 gene, established the transient SCC-iCre transgenic mice model by using R26R system to study the promoter activity of human CYP11A1 gene in the brain.

为了证明内生性P450scc在这些脑区的表现，本研究利用免疫组织染色分析，说明了在小鼠海马回结构，间脑内的上视丘疆核、视丘神经核、下视丘神经核，与新皮质等区域中皆可侦测到，且主要表现在神经细胞。

Fine mapping of Helminthosporium turcicum resistance gene Ht2 is extremely valuable for map-based cloning of the Ht2 gene,gaining a better knowledge of the distribution of resistance genes in maize genome and marker-assisted selection in maize breeding.

精细定位玉米大斑病抗性基因Ht2对于图位克隆该基因和探讨玉米基因组中抗病基因的分布规律及分子标记辅助选择有重要意义。

OBJECTIVE: To compare the differences of gene expression and the response to bFGF of human umbilical cord CD34+ and CD133+ cells, and to explore gene expression changes of bFGF-induced umbilical cord CD34+ and CD133+ hematopotic stem cells/hemapoietic progenitor cells in vitro.

目的：利用基因芯片比较脐血CD34+和CD133+细胞基因表达的差异，并探讨碱性成纤维细胞生长因子体外诱导脐血CD34+和CD133+造血干/祖细胞分化的基因表达变化，及其对碱性成纤维细胞生长因子反应性差异。

To investigate the clonal rearrangements of immunoglobulin heavy chain gene and T-cell receptor γ gene in peripheral blood cells of patients with hemapoietic malignancies and their significance, the clonal rearrangements of IgH and TCRγ genes were detected by polymerase chain reaction in peripheral blood cells of patients with hemapoietic malignancies, including 32 cases of non-Hodgkin lymphoma, 18 cases of acute myeloid leukemia, 24 cases of multiple myeloma, 8 cases of acute lymphoblastic leukemia and 6 cases of chronic lymphoblastic leukemia.

检测各种血液系统肿瘤患者外周血细胞免疫球蛋白重链基因和T细胞受体γ基因克隆性重排并探讨其意义。通过多聚酶链式反应方法检测32例非霍奇金淋巴瘤、18例急性髓性白血病、24例多发性骨髓瘤、8例急性淋巴细胞白血病及6例慢性淋巴细胞白血病患者外周血细胞IgH及TCRγ克隆性基因重排。

ConclusionHCMV infection significantly down-regulated the expression of HOXB6 gene.The result suggests that the abnormal expression of HOXB6 gene induced by HCMV may play an important role in HCMV-induced abnormal hematogenic damage.

结论HCMV感染能诱导脐血红系祖细胞HOXB6的表达下调，HCMV有可能通过调控HOXB6基因表达异常而引起红系祖细胞的增殖障碍。

The constructed recombinant adenovirus can mediate the human TPO gene transfer efficiently and can be used in the gene therapy of hemopoietic reconstruction.

所构建的重组腺病毒能有效介导人TPO基因的转移，可望用于造血功能重建的基因治疗研究。

Further, the global gene expression profiles differ in hepatoma cells with and without HBV gene expression and replication.

此外，在肝癌细胞系中，乙肝病毒的复制和乙肝蛋白的表达能引起细胞基因表达的全局性改变。

Plant traits were categorized to three classes: the first class traits were dominated by major gene with little genetic effect of polygene, including days from sowing to flowering, days from sowing to boll opening, lint percent, days from squaring to flowering, days from flowering to boll opening and plant bolls. The hereditability of major gene and polygene were about 36. 85%～66. 79% and 2. 83%～12. 64%, respectively.

通过对短季棉早熟及其相关性状的主-多基因综合分析发现：主基因普遍存在，各时期至少有一对主基因起主要作用，同时受多基因的修饰，在短季棉发育的不同时期，其主基因的对数、作用效果、方向以及主、多基因的遗传率均不同。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。