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Introducing into to wheat the gna gene that encodingsnowdrop lectin(Galanthus nivalis agglutinin;GNA),a toxic protein to Homoptera,or cryIA gene that encoding Bacillus thuringiensis insecticidal protein forLepidopteraby genetic transformation will produce new germplasm that maybe produce resistant to somekinds of pests.

利用转基因技术，将对同翅目昆虫具有抗性的雪花莲凝集素基因gna和对鳞翅目昆虫具有抗性的苏云金芽孢杆菌杀虫蛋白基因cryIA导入小麦，极有可能获得对某些害虫具有一定抗性的小麦新种质。

However, the mutation of GATA site at -2948bp remarkably reduced the reporter gene activity driven by SV40 promoter, but not by ε-globin gene proximal promoter. To test whether those sites act synergistically, we mutated 2 of these three binding sites with different combinations.

然而，GATAb（在-2948bp的GATA位点）的突变明显地降低了SV40启动子介导的报告基因转录活性，但是对ε-珠蛋白基因启动子介导的报告基因表达却影响不大。

In the present study C-mos nuclear and 12S rRNA mitochondrial DNA were employed as genetic markers to deduce the interrelationships among Gekkonidae lizards; Cyt b and ND2- tRNA~ gene sequences were used to discuss the population structure of Hemiphyllodactylus yunnanemis; 12S rRNA gene sequences were used to reveal the phylogenetic relationships among seven species of the genus Gekko.

基于12S rRNA基因全序列及C-mos基因序列片段探讨了壁虎科蜥蜴的属间关系；用Cyt b基因全序列和ND2-tRNA~基因片段初步研究了云南半叶趾虎的种群结构；用12S rRNA基因全序列探讨了中国壁虎属七物种间的系统关系。

Western blot and enzymatic assay indicated that the HL gene and germin gene was expressed in tobacco cell respectively.

Western杂交和草酸氧化酶活性检测分别证明了双价基因中的HL和germin在烟草中的表达。

A pair of primers were designed according to the germin gf-2.8 gene sequence reported in GenBank.Germin gene was isolated from the genomic DNA of wheat "Xiangmail3" by PCR amplification.

根据GenBank中的germin gf-2.8同源序列设计引物，通过PCR扩增，从湘麦13基因组DNA中得到了长度为812bp的目的片段，将PCR产物克隆到pDive载体上并测序。

Full-length cDNA of Gibel carp pou2 gene was cloned from gastrula SMART cDNA library through RACE-PCR. It consists of 2421 bp, with an ORF of 1416 hp encoding a protein of 471 aa. The complete amino acid sequence shares 91.0% identity with zebrafish pou2 gene product.

用RACE-PCR方法从原肠期SMART文库中扩增到银鲫pou2基因的全长cDNA，其全长为2421bp，开放阅读框为1416bp，编码471个氨基酸，与斑马鱼pou2基因的氨基酸序列一致性高达91.0%。

The high identity of hira gene ORF in gibel carp and colored crucian carp indicates that the function distinction may be caused by the expression distinction regulated by regulation region of gene.

由于彩鲫是两性生殖方式鱼类，在受精前hira基因大量表达，可能为受精时

Gl_2~e is a gene controlling glandless traits in cotton plant and seed, which is an important gene resource for gossypol-free cottonseed breeding.

Gl_2~e基因是控制棉花植株和种子无腺体的一个基因，是低酚棉育种中一个重要的基因资源。

MSCs were cultured in a conditional medium in subculture including: dexamethasone, vitamin C, and β-glycerophosphate. At the same time, we constructed the eukaryotic expression vector containing VEGF165gene. VEGF165 gene was transfected into MSCs by means of LipofectAMINE?2000. The stably gene expressive cells were screened with G-418 for 14 days.

在脂质体介导下将构建成功的真核表达载体pcDNA3.1- VEGF165转染到诱导培养的MSCs，G-418筛选阳性细胞克隆，将阳性克隆细胞和未转染的MSCs共同接种于β-磷酸三钙上，体外培养7天后，植入原大鼠肌肉内。

This papersummarizes the studies on glycinin including components,structure and gene families.The homogeneity of the glycinin gene sequences was compared by bioinformatics.Heredity Distances of the six glycinin genes were analyzed. Special emphasis is laidupon comparing the sequences and components of amino acids in the glycinin. Thecontents of major amino acids in the intact glycinin and in the acid and basic peptideswere analyzed. In view of others and our researches, we advance some new thinkingand tactics of improving rice nutritional quality by using glycinin genes.

本文在简要介绍大豆球蛋白组分、结构及基因家族后，采用生物信息学方法分析比较了不同种类大豆球蛋白基因之间的序列同源性，并对不同大豆球蛋白基因的遗传距离作了分析；本文还重点比较分析了不同大豆球蛋白的氨基酸序列和组成，以及不同酸性肽和碱性肽中重要氨基酸的含量，基于对前人工作的总结和我们的分析结果，本文提出了利用大豆球蛋白基因进行水稻营养品质改良研究的新思路和新策略。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。