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It has been thought that the complicated gene-gene interaction, heterogeneity of clinical presentation and numerous farraginous experimental data make it more difficult to draw a firm conclusion with genetic research work.

这主要是因为精神分裂症临床分型复杂，症状多样，若将不同类型的患者混杂起来研究将难以对这种异质性疾病作出可信的结论；同时该病作为多基因病的一种，其发病是由许多微效基因共同作用决定的，若孤立地研究某个候选基因或候选染色体区域与疾病的关系，也难以得出满意的结果；而且在研究结果的分析中，某个基因对发病的微小贡献将被&淹没&在巨大的数据信息中，难以提取。

Abstract］ Background and purpose:The APC gene and β-catenin gene play import roles in Wnt pathway and their abnormalities could be the cause for the tumorigenesis of desmoid-type fibromatosis.

摘要］背景与目的：APC和β-catenin基因作为Wnt通路上重要的成员，其异常在肿瘤的发生、发展过程中起着重要作用。

Key words］desmoid-type fibromatosis;adenomatous polyposis coli gene;β-catenin gene;denaturing high performance liquid chromatography

关键词］韧带样型纤维瘤病；APC基因；β-catenin基因；变性高效液相色谱分析

To investigate the disorder of Wnt signal transduction pathway in tumorigenesis, we studied the adenomatous polyposis coli gene and β-catenin gene mutations in desmoid-type fibromatosis.

本研究分析了韧带样型纤维瘤病中APC和β-catenin基因突变，探讨Wnt通路中APC/β-catenin基因异常在肿瘤发生中的作用。

The 7.4 kb DNA fragment containing the S. Choleraesuis std fimbrial gene cluster was PCR amplified and cloned into the pCR-XL-TOPO vector. The open reading frames of the std fimbrial gene cluster were analyzed. The resulting recombinant DNA was transformed into Escherichia coli HB101 strain.

本实验室藉由PCR方式，增幅7.4 kb的std线毛基因组，将其选殖至pCR-XL-TOPO质体DNA，委外定序后，分析std线毛基因组之开放性读框，进而将此重组DNA送入大肠杆菌HB101，并藉由RT-PCR及西方点墨试验观察到S。

In the genome of Klebsiella pneumoniae NTUH-K2044, we found nine fimbrial gene cluters including fim, mrk, and seven novel fimbrial operons, namely kpa, kpb, kpc, kpd, kpe, kpf, and kpg. These fimbrial gene clusters were cloned into the expression plasmid pET30b and then transformed to an afimbriated E.

在克雷白氏肺炎杆菌NTUH-K2044的基因体中，藉由HMMER找到了九套纤毛基因组，其中包含已知的第一型纤毛fim基因组和第三型纤毛mrk基因组，以及七套未曾被报导的纤毛基因组，分别被命名为kpa、kpb、kpc、kpd、kpe、kpf和kpg。

The comparative analysis of electronic expression profile of zfand5a gene from zebrafish, house mouse and pig that has high sequence homologies with zjp123 gene from P. olicaceus were carried out. Several conserved domains and motifs were discovered by the primary and secondary structure analyses. Zinc fingerlike tertiary struclure "C-Xasubscript (2-4-C-X11-C-X2-C" was shown by homology modeling.

电子表达谱分析分别对与ZFP123有较高同源性的斑马鱼、家鼠和猪的zfand5a(zfp123)基因各个组织表达情况进行了比较分析；一、二级结构分析发现其具有多个保守的基序和结构域；同源建模显示其具有一个&C-X下标(2-4-C-X11-C-X2-C&结构的锌指样三级结构。

In this research, FvndhB gene segment was obtained, and the phylogenetic analysis was researched. It established basis on cloning the span of FvndhB and studying the characteristics of FvndhB gene expression in Fraxinus velutina Torr.

成功获得了绒毛白蜡FvndhB基因片段，并对其进行了同源性分析，为进一步克隆绒毛白蜡FvndhB全长基因及分析其表达特性奠定了基础。

In this research, FvndhB gene segment was obtained, and the phylogenetic analysis was researched. It established basis on cloning the span of FvndhB and studying the characteristics of FvndhB gene expression in Fraxinus velutina Torr.

本研究首次从绒毛白蜡中克隆了ndhB基因，并对其进行了同源性分析，为进一步克隆绒毛白蜡ndhB全长基因及分析其表达特性奠定了基础。

An expression vector was constructed with a safe marker gene, 6-phosphomannose isomerase, and an insect-resistant gene, Galanthus nivalis agglutinin, was used in rice genetic transformation in the present study.

采用农杆菌介导法将来源于大肠杆菌的6-磷酸甘露糖异构酶安全选择标记基因和雪花莲凝集素抗虫基因构建于同一表达载体上进行遗传转化，获得带有安全标记基因的抗褐飞虱转基因植株。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。