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Results We can induce MSCs into osteoblasts which can express gene col Ⅰ and ALP. Through analysis growth curve, we demostrateed the diphosphonate or assemble flavone of drynaria rhizome with 10^(-4)~10^(-6)mmolL^(-1) had the inhibition on the osteomast; it with 10^(-8)mmolL^(-1) had a promotion on the osteoblast; it with 10^(-10)~10^(-12)mmolL^(-1) had a restraint on osteoblast. Conclusion Through gene analysis, we found the diphosphonate or assemble flavone of drynaria rhizome can have the promotion on the osteoblast.

结果 骨髓基质细胞经过4周诱导可转化为表达colⅠ和ALP的成骨细胞，经过生长曲线分析，二磷酸盐和骨碎补总黄酮10^(-4)~10^(-6)mmolL^(-1)时，对诱导后的成骨细胞有抑制作用；10^(-8)molL^(-1)有促进作用；10^(-10)~10^(-12)mmolL^(-1)不明显，其中10^(-8)mmolL^(-1)作用最强；基因分析后，发现二磷酸盐和骨碎补总黄酮促进成骨细胞表达colⅠ和ALP，且两者合用效果大于单独用药。

According to the classical signal transduction pathway of estrogen, we use Luciferase reporter gene to screen phytoestrogen form Selaginella tamariscina, Pinus massoniana Lamb, Corallodiscus flabellate, Dryopteris sublaeta Ching et Hsu and Leonurus heterophyllus. In this study,the pβgal-Control vector, the pERE-TAL-luc vector, the pCNX2-hERα/pCNX2-hERβvector were transfected into HEK293 cell line. If the components in the herbs combine with the ER, then it could induce the expression of reporter gene controlled by ERE.

根据体内的雌激素信号转导途径，我们选用了以luc为报告基因、ERE为调控序列的重组报告基因载体pERE-TAL-luc，并将其与重组人ERα(humanERα，hERα)表达载体pCXN2-hERα和重组人ERβ(humanERβ，hERβ)表达载体pCXN2-hERβ共转染ER阴性细胞系，建立了靶向ERα或ERβ的细胞水平药物筛选模型，结合小鼠子宫增重实验，对卷柏、松针、益母草、石胆草及贯众等5种中药的植物雌激素作用进行了研究，以期为临床防治更年期综合症等提供准确、科学的实验基础。

Objective To analyze the relationship between genotype and phenotype of vitelline macular dystrophia (VMD2) gene in a family with Best disease, and to provide the theoretical basis for gene diagnosis of Best disease.

目的观察Best病家系卵黄样黄斑营养不良基因(VMD2)基因型与表现型之间的相互关系，为建立Best病基因诊断方法提供理论依据。

Objective: To identify EDA gene mutation in a pedigree of Han nationality in Xinjiang with X-linked hypohidrotic ectodermal dysplasia so as to provide a basis for gene and genetics diagnosis of this disorder.

目的：检测一新疆汉族X连锁少汗性外胚叶发育不全家系引起先天缺牙的EDA基因突变，为XLHED综合征的基因和遗传学研究提供理论依据。

Objective: To explore the inhibition effects of ectogenic wild-type p53 cDNA (Ad-wtp53) on colorectal carcinoma cell lines with different p53 gene status and search for the role of wild type p53 tumor suppressor gene in occurrenc and progress of malignant tumor.

目的：探索重组腺病毒介导的外源性野生型p53cDNA(Ad-wtp53)对内源性p53基因状态不同结直肠癌细胞的抑制作用是否存在差异，研究p53基因抑制肿瘤细胞增殖的机制。

The homology analysis showed that these 5 fragments had very high homologies with the maize mRNA that hade been separated . S_ had 80% homology with cytochrome b5 gene of N.tabacum . SCT-TG had over 90% homology with α-tublin gene of Z.mays , Eleusine indica, Hordeum vulgare, Oryza sativa and Setaria viridis.

序列同源性分析表明：这 5 条片段都与已经分离的玉米 mRNA 有很高的同源性，S_与烟草细胞色素 b5基因有 80%的同源性、SCT-TG与玉米、牛筋草、大麦、狗尾草、水稻等的α-微管蛋白基因有 90%以上的同源性。

To solve the problem and improve the safety of gene therapy, we used microencapsule, a insulation tool of cell transplantation immunity, to encapsule human fibroblastlike bone marrow stroma cells transfected by part of CEA gene and produced microencapsulated CEA transgeneic cell vaccines; we also studied the expression of vaccine in mouse body as well as its immunologic characteristics.

为解决上述问题、提高基因治疗的安全性，本研究采用细胞移植免疫用隔离工具-微胶囊包裹转染部分CEA基因的人成纤维样骨髓基质细胞HFCL，制备出CEA微囊化转基因细胞疫苗，并进行了疫苗在小鼠体内的表达及其免疫学性质的研究，探讨该疫苗应用于CEA阳性肿瘤治疗的可能性，并为进一步的临床应用研究奠定了基础。

The embryonic analysis results indicated that the disruption of oxt gene would lead to the disruption of wg transcription and the expression of engrailed gene, as the wg transcription is dependant on the Engrailed.

进一步结合运用FLP-FRT系统，用缺损GFP蛋白的表达来标记纯合的oxt突变基因克隆，针对眼睛成虫盘的克隆分析结果表明，在oxt突变克隆中，Hh下游的Ci蛋白的表达区域完全消失。

Methods Gene analysis by analysis susceptibility, ERIC-PCR techniquea and plasimid fingerprinting were put into practice for the detection of gene variation of 10 strains of Salmonella enteritidis. The similarities between different strains were obtained by software NTSYS-PS 2.0 for cluster analysis.

药敏试验采用K-B法，用碱裂解法提取细菌的质粒DNA，采用肠杆菌重复基因间隔重复共有序列技术对10株肠炎沙门菌进行基因分析，运用NTSYS-PC2.0软件进行同源性比较，聚类分析。

This gene regulation is accomplished by epigenetic processes that control gene expression without altering DNA.

这是基因调控是由控制基因的表观遗传完成的，并没有改变DNA。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了