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In one word, the sequence analysis, gene transferation and its fluorescent protein chimeric genes study of the IPL from Dasyatis akajei would facilitate the further investigation of the structure-function relationship and the exact phospholipid signal transduction mechanisms of the gene.

总之，赤魟IPL的序列分析、基因转染及其荧光蛋白嵌合体的研究，为进一步研究其结构与功能关系及磷脂信号传导机制打下了基础。

Comparabilityof some segments reached about 86% to 90% including Arabidopsis thaliana CIPK7、Arabidopsis thaliana At3g23000 gene、Arabidopsis thaliana SNF1 related protein kinase(At3g23000; MXC7.3) mRNA、Cucumis sativus RBL1 mRNA, complete cds、Ranunculusmacranthus ribosomal protein L14 (rp114) gene, partial cds、Daucus carota chromasomecomplete genome and so on. There are also some other segments had a low identity andconsidered as unknown genes.

对部分差异条带进行了克隆测序，登陆GENEBANK对差异cDNA片断的序列进行核苷酸及氨基酸的同源性对比，其中一些片断与拟南芥CIPK7、拟南芥At3g23000基因、拟南芥SNF1相关蛋白激酶mRNA、栽培黄瓜RBL1mRNA全序列、毛茛核糖体蛋白L14基因、胡萝卜染色体基因组等均有86％-90％左右的同源性，还有部分片断的同源性很低，可能为未知基因序列。

In order to achieve biological remediation of the pollution and improve the efficiency of treating refractory wastewater, purposive acceleration of the horizontal gene transfer of specific degradative gene will be a new concept to solve these problems.

为了实现污染地区的生物修复及强化难降解废水处理的效果，人为的制造和加快特定降解基因的水平转移被认为是解决这些问题的一个新思路。

The dermoid sinus gene is believed by some to be recessive, meaning that the animal must receive one defective gene from each parent in order to develop the condition.

有人认为皮窦基因是隐性的，也就是犬只必须从父母双方各获得一个皮窦基因才可能导致疾病发生。

Methods The NGF, BDNF, and NT3 genes of rats were cloned, the eukaryote expression vectors were established, the three kind of recombinant vectors were used to transfect astrocytes, the positive cloned cells were cultured dilatedly after G418 sifting; using supernates of culture liquid of astrocytes modified by gene to culture PC12 or TrkB-PC12, the expression and its level of gene target cells aimed genes were measured by Western blotting or immunohistochemical method.

克隆大鼠NGF、BDNF和NT3基因，构建真核表达载体；3种重组载体分别转染星形胶质细胞，G418筛选后获得阳性克隆细胞进行扩大培养；取基因修饰星形胶质细胞培养液上清培养PC12细胞或TrkB-PC12细胞；用Western blotting杂交或免疫组织化学方法检测基因靶细胞目的基因的表达及其水平。

Ten transformants and 10 conjugons showed high-level resistance against aminoglycoside antibiotics,all of which the value of MIC＞256μg/mL carried armA gene.Conclusions:The Ab clinical isolates from burn ward in Gansu Province People\'s hospital have high drug-resistance.16S rRNA methylases gene exsist in Ab and locate in plasmid chromosome.They are easily disseminative,which demonstrated a very high level of resistance to various aminoglycosides.

甘肃省人民医院烧伤病房鲍氏不动杆菌临床分离株有非常高的耐药率，16S rRNA甲基化酶基因armA在鲍氏不动杆菌中存在，且位于质粒染色体上，易于传播和扩散，导致所有氨基糖苷类抗菌药物高水平耐药。

Spp.. The melA locus is composed of a divergently transcribed α-galactosidase gene and a gene coding for a putative transcriptional regulator of AraC/XylS family.

halodurans 的 melA locus 是由一α-galactosidase 基因 melA 及其上游反向属於 AraC/XylS family 的转录调节基因 melR 所组成。

The GLDH gene expression showed a continuing reinforcing trend after morphine injection compared with control group, even after drug withdrawal. The ADA mRNA contents in morphine group Ⅰ,Ⅱ and withdrawal group Ⅰ,Ⅱ were 2.65,1.89,1.18, and 0.87-fold of that in control group dividedly. The ADA gene expression increased after morphine injection and fell gradually after withdrawal.

结果：吗啡Ⅰ、Ⅱ组及停药Ⅰ、Ⅱ组大鼠肝脏GLDH mRNA含量分别为对照组的1.02、1.09、1.16及1.46倍，吗啡给药大鼠肝脏中GLDH基因表达随给药时间延长而持续增强，自然停药一定时间内大鼠肝脏中GLDH mRNA含量仍持续增高；吗啡Ⅰ、Ⅱ组及停药Ⅰ、Ⅱ组大鼠肝脏ADA mRNA含量分别为对照组的2.65、1.89、1.18及0.87倍，吗啡Ⅰ、Ⅱ组大鼠肝脏ADA的基因表达均比对照组明显增高，自然停药后大鼠肝脏ADA表达逐渐下降，停药7 d时下降至接近对照组水平。

Another presentation at the meeting reported by members of the same research group indicated that TBX15 may also play a role in inhibiting adipocyte development and fat accumulation."Given these findings, it is reasonable to assume that a genetic variant in this gene may predispose a person to obesity by decreasing the activity of this gene," noted Dr. Doria.

另一项研究简报是由同组研究团队的成员所进行，其中指出TBX15可能也影响抑制脂肪细胞的形成及脂肪堆积；Doria博士提及，就这些研究发现观之，推论此基因中的一种基因变异，致使基因活动力下降而导致肥胖产生是合理的。

In this research work, the technology of microbial conversion was studied in order to establish an environment friendly method for the production of doxorubicin. A doxA gene encoding Daunorubicin C-14 hydroxylase was cloned from a daunorubicin-producing strain Streptomyces coeruleorubidus SIPI 1482. Some plasmids for the expression of doxA gene were constructed and transformed into S.

本研究对微生物转化法替代现有工艺生产阿霉素进行了探索，从柔红霉素产生菌天蓝淡红链霉菌SIPI 1482中克隆了柔红霉素C-14羟化酶基因，构建了doxA基因的链霉菌表达质粒，导入变铅青链霉菌TK24中获得了柔红霉素转化基因工程菌，并研究了doxA基因在工程菌中的表达，最后还对工程菌转化柔红霉素生成阿霉素的发酵工艺进行了初步研究。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。