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Floral organ determination is best explained by the ABCDE model postulated by genetic studies of Arabidopsis thaliana. Sepals are determined by A and E class genes; petals are determined by A, B, and E; stamens by B, C, and E; and carpels by C and E class genes. A, B, C, and E class gene lineages are known having duplicated several times during the evolution of angiosperms. One of the noted major duplication events occurred in the origin of the early angiosperms, leading to the formation of subgroups of B/C/D/E class. Another one occurred near the basal eudicots and gave rise to further subgroups in A/B/C/D/E class genes among core eudicots. The phylogenetic position of the family Buxaceae is located right where the second major duplication of ABCDE genes might have occurred, which is supported by multiple gene (nuclear 18S rDNA, chloroplast rbcL and atpB) phylogenetic analyses.

目前经由模式植物阿伯芥的研究，建花部器官决定基因的调控，即花萼由A、E 群基因共同决定，花瓣由A、B、E 群基因，雄蕊由B、C、E 群基因，而心皮由C 和E 群基因决定。A、B、C、E 四群基因在被子植物的演化过程中发生过次的复制事件，其中比较重要的一次发生在早期被子植物演化出之时，形成B/C/D/E 群基因的次系群；另一次复制事件则发生在真双子植物基群附近，形成仅於核心真双子植物的A/B/C/D/E 群基因之次系群。

The mutation of K-ras gene and the abnormal transcription of FHIT gene have close correlatetion with tumorigenic development of lung cancer.K-ras and FH1T became the molecule target of smoke cancerigenic.

K-ras、FHIT基因异常与肺癌发生密切相关。

The caprine IL-2 gene and the IFN-γgene without the signal peptidesequence were subcloned into (5") Bam H I and (3") HindⅢrestriction sites of pET30aplasmid, the recombinant plasmid were transformed into E. coli BL21(DE3) and induced withIPTG, they were demonstrated by SDS-PAGE that the genes were expressed as inclusion bodies.

应用DNA重组技术，将IL-2基因和去除信号肽的IFN-γ基因分别插入到原核表达载体pET-30a的BamHⅠ和HindⅢ位点上，构建原核表达质粒pET30a-CapIL-2和pET30a-CapIFN-γ，转化大肠杆菌BL21(DE3)，经IPTG诱导后进行SDS-PAGE电泳，结果表明目的基因在大肠杆菌中以包涵体形式融合表达。

AY506804 gene from apsella bursa-pastoris by rapid amplification of cDNA ends . The full-length cDNA of Capsella bursa-pastoris ICE1 gene (Cbice53) including complete 5'-UTR and 3'-UTR was 1811bp long, containing a 1476bp open reading frame which encodes a 492 amino acids protein.

本研究在荠菜还得到了一个新的ICE1基因（基因bank登录号：AY506804），该基因（Cbice53）全长cDNA为1811bp，含有一个1476bp的开放阅读框，预测编码一个492个氨基酸的蛋白。

A new CBF (Genbank accession no.: AY391121) gene was cloned from Gapsella bursa-pastoris by rapid amplification of cDNA ends . The full-length cDNA of Capsella bursa-pastoris CBF gene was 1034bp long and contained a 657bp open reading frame encoding a putative DRE/CRT -binding protein of 219 amino acids.

本文克隆得到了荠菜Cbcor15b转录激活因子Cbcbf（基因bank登录号：AY391121）全长cDNA为1034bp，含有一个657bp的开放阅读框，预测编码一个219个氨基酸的DRE/CRT的结合蛋白质。

Part IIMorpholino-mediated translational inhibition and overexpression oftbx2 in zebrafish cardiogenesis Objective To elucidate the role of tbx2 in zebrafish cardiogenesis via morpholino-mediated translational inhibition and overexpression on the base of expression pattern of tbx2. Methods Gene-specific antisense morpholino oligonucleotides were designed to against ATG region of zebrafish tbx2 to knockdown endogenous tbx2 gene.

设计tbx2-MO和对照MO，在斑马鱼受精卵的单细胞期，进行不同浓度的显微注射，并通过荧光蛋白融合标记实验和tbx2-mRNA、tbx2-MO共注射拯救实验，验证tbx2-MO抑制tbx2基因表达的有效性和特异性；基因过表达是通过给斑马鱼受精卵注射体外合成的tbx2-mRNA，来研究tbx2基因功能获得对斑马鱼胚胎心脏发育的影响。

Knockdown of the gene blocked neural differentiation and the effect was rescued by the wild-type human gene, and not by a catalytically inactive mutant.

研究还发现，KDM7A表达抑制所引起的干细胞神经分化受阻现象可被FGF4的过表达所逆转，表明FGF4作为被KDM7A调控的基因介导了KDM7A在胚胎干细胞神经分化中的功能。

We composed the rhoa-gene of Wistar rat with the plasmid of pAdTrack-CMV to get the recombinant plasmid. On the basement, we can use the catholyte lipofetamine to transfected the neural stem cells , In the following, we observe the expression of the rho-gene and the geng how to act the motor neuron and NSCs.

本试验用克隆出的大鼠rhoa基因构建到质粒pAdTrack-CMV中，获得重组质粒pAd-rhoa，用阳离子脂质法介导转染到神经干细胞，观察目的基因的表达及其对运动神经元和神经干细胞的作用。

While the control group had a horizontal curve which had micro changes. To compared with control group, the serum hepcidin contents had a tendency like a cavate parabola which had the lowest on 8th、9th、10th day, the same to the hepatic hepcidin gene expression changes. While control group had the same changes of gene expression .

血清铁调素ELISA检测含量趋势与对照组比较，呈现凹陷的抛物线变化，低值出现在第8、9、10天，整体变化趋势与肝脏基因水平相符合；对照组变化亦与肝脏hepcidin基因水平相符合。

DNA sequence data of the rbcL gene, the matk gene, and the internal transcribed spacers of nuclear ribosomal DNA involving 14 species representing the Cercidiphyllaceae. Eupteleaceae. Platanaceae, Tetracentraceae. and all the subfamilies of the Hamamelidaceae have been investigated in the present study.

本研究测定了包括连香树科、领春木科、悬铃木科、水青树科和金缕梅科内所有亚科代表共计14个种的rbcL基因，matK基因和18S-26S核核糖体内转录间隔区的序列，并结合原有资料对所有低等金缕梅类及其相近类群进行了分支分析。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了