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TPP gene plays an important role in synthesis of trehalose. The cloning of TPP gene lays a solid basis for investigating the biosynthesis of trehalose in L. bicolor.

海藻糖－6-磷酸磷酸酯酶在海藻糖合成过程中起关键作用，该基因的克隆为研究海藻糖在二色补血草中的合成奠定了基础。

Are two varieties of species B.oleracea. Cauliflower bobcal gene loses its original function due to its terminated mutation while cabbage BoCAL gene has its complete function.

是芸薹属甘蓝种的两个变种，前者的bobcal基因由于基因的终止突变失去了原有的功能，而后者的BoCAL基因具有完整的生物学功能。

All the segregation indicated that BoCAL gene had complemented to cauliflower demestic bobcal gene.

说明BoCAL基因对花椰菜的内源bobcal基因有互补性。

The introduction of cabbage BoCAL gene into cauliflower may bring great influence to the expression of the mutated bobcal gene and cause the alteration of curd phenotype.

将甘蓝BoCAL基因转入花椰菜，有可能对突变的bobcal基因的表达有重要影响。

PCR , Southern blotting and selection on kanamycin medium indicated that BoCAL gene had single-sited integrated into cauliflower and conformed to the segregation of single-dominant gene by the law of Mendelian Inheitance.

PCR、Southern杂交和抗卡那霉素筛选表明，外源BoCAL基因已经转入受体植物并且是单位点插入，并且符合孟德尔单显性基因的遗传。

Results ①DNA sequencing showed that pBX1 contained a 477bp inserted gene fragment,and when it was compared with the published sequence of the specific region of the gene of the 83kd antigen protein from Borrelia burgdorferi B31 strain,only one amino acid codon was different.

结论该研究成功地对莱姆病螺旋体83kd抗原蛋白特异性区段进行了基因重组和表达，为进一步研究其在莱姆病血清学诊断和基因工程亚单位疫苗研制中的应用奠定了基础。

The results were the basic information to further study SNPs of MC1R gene associated with plumage color on mule duck. By using the program of Blast on GenBank, the results indicated that duck had homology with 98.4% homology with branta bernicla at nucleotide level of MC1R gene, and 92.8％～98.2% homology with other birds.

为进一步研究半番鸭羽色MC1R基因单核苷酸多态性奠定了基础；通过BLAST进行相似性搜索，结果表明，鸭与黑雁MC1R基因的核苷酸序列同源性最高为98.4％，与其他家禽的同源性也保持在92.8％～98.2%之间。

We obtained complete coding sequence of myostatin gene from Bos taurus, Bos indicus, Bos grunniens, Bos frontalis and Bubalus bubalis (swamp buffalo and river buffalo) by PCR fragments sequencing method. The molecular evolution characters of CDS of myostatin gene were analyzed.

运用PCR分段扩增测序法，获得普通牛、瘤牛、牦牛、大额牛、水牛MSTN基因编码区全序列，进而分析编码区序列的分子进化特征。

In the lens regeneration of Bufo raddei Strauch, The mRNA of Pax-6 gene expression was positive at 7 day, 30 day during lens regeneration. The gene of Pax-6 was positive during the different time of lens development and regeneration.

晶状体再生的第30天晶状体纤维开始分化，荧光定量的结果显示再生30天比21天Pax6基因的表达量高，表明在此时期Pax-6基因对再生的过程中调控晶状体纤维分化方面起到了关键的调控作用。

However, the first one enzyme 1-deoxy-D-xylulose 5-phosphate synthase gene and the second one enzyme 1-deoxy-D-xylulose 5-phosphate reductoisomerase gene in MEP pathway had been reported and expressed in the latex of rubber tree, indicating that MEP pathway may be involved in rubber biosynthesis.4 - hydroxy -3 - methyl -2 -- butenyl -4 - phosphate reductaseis the last enzyme that catalytic 4 - hydroxy -3 - methyl -(2E)- butenyl -4 - phosphate to generate isopentenyl pyrophosphate.

然而已有报道MEP途径中第1个酶1-脱氧-D-木酮糖-5-磷酸合成酶（1-deoxy-D-xylulose 5-phosphate synthase，DXS）基因和第2个酶1-脱氧-D-木酮糖-5-磷酸还原酶（1-deoxy-D-xylulose 5-phosphate reductoisomerase ，DXR）基因在橡胶树胶乳中的表达，这表明MEP途径可能参与橡胶的生物合成。4-羟基-3-甲基-2--丁烯基-4-磷酸还原酶（4-hydroxy -3-methyl-2--butenyl-4-diphosphate reductase，HDR）是异戊烯基焦磷酸合成途径之一甲基赤藓糖磷酸（methylerythritol phosphate，MEP）途径中的最后一个酶，催化4-羟基-3-甲基-(2E)-丁烯基-4-磷酸生成异戊烯基焦磷酸。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。