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The 1 kb scaffold-attachment region at 5' non-transcription region of rRNA gene of silkworm Attacus ricini was cloned into eukaryotic expression vector pLu, which contained luciferase report gene and neo selecting marker.

蓖麻蚕核糖体RNA基因非转录间隔区有一长约1kb的核骨架结合区(scaffold-associated region，SAR)。

The cDNA document of ACC oxidase gene of Citrus aurantium L. was cloned. By using the program of BLAST on NCBI GenBank database, the sequence presented a very high match with the ACC oxidase genes from other plants. The base sequence was analysed by using biology programe of DNAStar 5.0. 278 amino acids were coded by the base sequence and the base sequence had the same conserve region of ACC oxidase gene of many kinds of other plants. The base sequences comparability was more than 72% compared with those of many kinds of other plants. The amino acid sequences comparability was more than 70% compared with those of a lot of other plants.

克隆了酸橙1-氨基环丙烷－1-羟酸氧化酶基因cDNA片段，将片段序列在NCBI网站上进行同源性搜索，显示的皆为不同植物的ACC氧化酶基因，因而认为所克隆的片段就是酸橙ACC氧化酶基因；并运用DNAStar5.0软件进行序列分析，推导的氨基酸序列为278个残基；具有所有植物ACC氧化酶基因共有的保守区域；与多种植物的ACC氧化酶基因的核苷酸和氨基酸序列的同源性都在72%和70%以上。

Based on the analysis of the sequence structure and expression characterization, we can conclude that the lac1 gene obtained from Auricularia polytrica is laccase gene.

结构分析和功能验证均表明：本研究获得的基因lac1 为漆酶基因。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

Here, a novel WRKY gene, OsWRKY52, was isolated from a rice cDNA library. This gene included an open reading frame of 1 719 bp in length, and the deduced polypeptide contained 572 amino acids,sharing 54％ identity with a WRKY1 protein from Avena sativa.

从水稻cDNA文库中分离到一个新的WRKY基因--OsWRKY52 cDNA，包括一个1 719 bp的开放读码框，推测编码一个由572个氨基酸组成的蛋白质，与燕麦AsWRKY1具有54％的氨基酸一致性。

The interaction between a plant resistance gene and the corresponding avirulence gene of the pathogen triggers a series of defense responses.

细胞主动参与的死亡即程序化死亡现象普遍存在于各种生物中，并贯穿于生物体生长发育的全过程。

One type of important plant disease resistance, gene\|for\|gene resistance, is resulted from the interactions between products of the pathogen avirulence genes and their matching plant resistance genes.

植物抗病基因与病原物无毒基因产物间直接或间接相互作用导致产生的基因对基因抗性是植物抗病性的重要形式。

An important part of this study was to deal with a avirulence gene mutant, MS220, which was obtained from the pool of transformants generated by SacⅠ mediated transformation and changed its avirulence into virulence on rice cultivar Aichiasahi (carrying resistance gene Pi-a).

本研究的一个重要组成部分是作者对从SacⅠ介导的转化群体中筛选得到一株对水稻品种爱知旭特异性发生改变了的突变体MS220进行分子遗传学分析。

These responses are controlled by a defense signaling network, which is activated by the recognition of a plant resistance gene encoded protein, R protein, and a pathogen avirulence gene encoded protein, Avr protein, in a model of the receptor ligand recognition.

这个信号系统由抗病蛋白和病原菌非毒性蛋白在一种配体受体的互作模式下激发，并由信号分子H2 O2 ，NO和系统信号分子SA ，JA和乙烯和通过关键调控基因传递和放大，最终诱导一系列防卫反应基因的表达和代谢的变化而产生抗性。

Objective To detect the abnormal YRRM gene in azoospermia and to explore the pattern of YRRM gene in Chinese men.

目的 探索无精子症因子基因的候选基因之一YRRM基因在无精子症患者中的异常，了解中国人群的YRRM基因的形式。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。