查询词典 Gene

The regulation of gene expression is to have relations to the assembly of the DNA binding proteins which bind to DNA to initiate the transcription. Some of these proteins have the ability to promote bending or looping of DNA. The FIS protein regulates gene expression in Escherichia coli by this property of bending DNA. There are many researches to discover the mechanism of FIS protein binds to DNA. However the experiment data only show the supposititious results and figure out the model of FIS-DNA complex structure. This research uses molecular dynamics simulation to compute the detail of the interaction between FIS and DNA. We observed FIS prominently affects the DNA bending by the type of local bending. Analyzing the DNA structure properties, roll and slide, also demonstrates the local bending type and region. The hydrogen bonds between FIS and DNA are analyzed to compare with the experiment data. Finally we compute the binding free energy between FIS and DNA to estimate the result of simulation and compare with the value of λ-repressor-DNA complex.

基因的表现与结合於DNA促使DNA转录作用起始的DNA结合蛋白组装有关，一些这类的蛋白质具有促使DNA扭曲或将DNA形成环状的能力，FIS蛋白质即是藉由扭曲DNA的特性调控大肠杆菌基因的表现，目前已有很多的研究发现FIS与DNA结合的机转，然而这些实验的数据仅能显示推测性的结果，进而利用这些结果推论出FIS与DNA聚合体结构的模型，本研究利用分子动态模拟的方式计算FIS与DNA交互作用的细节，我们观察到FIS主要影响DNA的扭曲方式为DNA区域扭曲的类型，并且分析DNA结构特性roll与slide也指出扭曲方式为区域扭曲与其扭曲的区域，我们也分析FIS与DNA之间的氢键，并且与实验的资料作比较，最后我们计算FIS与DNA之间的结合自由能评估模拟的结果，并且计算出的值和λ-repressor与DNA聚合体结构的实验值作比较。

The results suggest that could be a new tumor suppresser gene in tumor gene therapy .

是一个较广谱的抑癌基因，可以作为肿瘤基因治疗的一个新目的基因，具有良好的应用前景。

The results suggest that RA538 could be a new tumor suppresser gene in tumor gene therapy .

是一个较广谱的抑癌基因，可以作为肿瘤基因治疗的一个新目的基因，具有良好的应用前景。

Objectives: The FHIT gene found by Ohta and others in 1996 is a new and uncertain tumor suppresser gene.

目的 FHIT基因是ohta等人于1996年发现的一个新的候选抑癌基因，其DNA序列和蛋白质结构目前已基本清楚。

At the same time, intact shuttle-plasmid pZ189 was also introduced into the host cell which was used for detection of nontargeted mutation frequency. After replicating in cells, progeny plasmid DNA was isolated and transformed into E coli. MBM7070.In the presence of ampicillin, X-gal and IPTG, pZ189-transformed E. Coli MBM7070 forms blue colony if the plasmid contains an active SupF suppresser tRNA gene, and white or light blue colony if the SupF tRNA gene is inactive.

但是由于检测非定标性突变需同时引入穿梭质粒pZ189在细胞中复制，并回收质粒DNA，通过转化宿主菌E.coliMBM7070，在含x-gal和IPTG的氨苄指示板上培养，如果用于检测突变的靶基因SupF tRNA基因发生突变，则菌落色泽为白色或淡蓝色，而正常者为兰色。

Objective To investigate the suppression effect of tumor suppressor gene p53 gene in lung adenocarcinoma cell lines.

目的探讨野生型p53基因及其表达产物p53蛋白在恶性肿瘤基因治疗中的作用。

Those aspects of the bad gene from the point of view on that susceptibility gene.

那些不好的的方面从基因的角度谈，就是易感基因。

A general trend in the research of symbiosis in ciliates is further exploration of the influence of the symbiont on the gene structure and gene expression of the host. The expected results will contribute to a better understanding of the regulatory mechanisms controlling the interaction,the exchange of substances between the host and symbiont at a molecular level, and the pattern of life processes sur rounding the relationship between cellular structures and functions in eukaryocytes.

作者推测，探索共生体对宿主基因结构及其表达产物的影响可能是对纤毛虫中共生作用研究的主要趋势，这对于深入了解真核细胞中宿主-共生体双方的相互作用、物质交流在分子水平上的调控机理、细胞结构与功能的关系等细胞生命活动规律是有意义的。

The Student's t statistic is used in one stage log-ratio models to identify differentially expressed genes.

本研究最后也透过一阶段对数比模型和gene-by-gene模型来讨论决定重复数的问题。

Taenia saginata asiatica's Spef1like gene was successfully cloned and its expression and fusion protein was purified,which provides favorite conditions for further studies of the gene.

成功克隆了亚洲牛带绦虫Spef1Like基因，并表达和纯化了Spef1Like蛋白，为研究Spef1Like的功能及其疫苗等研究提供了基础依据。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。