查询词典 Gene

Objective In order to make a foundation for molecular diagnosis and gene engineering studies of Avian encephalomyelitis virus, the structural protein VP3 gene of AEV and expression of its recombinant protein were cloned and sequenced.

目的对VP3进行克隆和测序后，在杆状病毒系统中表达获得重组蛋白，为AEV的分子诊断以及更深一层次的分子和基因工程研究打下基础。

Accordingto the difference of HA gene,which is separated 15 stype from H1 to H15;according to difference ofNA gene,which is separated 9 stype from N1 to N9.It is no cross-reaction between different HA orNA,so it is diffcult to detective.

根据流感病毒的血凝素和神经氨酸酶的抗原性差异，可将A型流感病毒分为不同的亚型，A型禽流感病毒的HA已发现16种，NA有9种，不同的H抗原或N抗原之间无交叉反应，给该病的防制带来了极大的困难。

Methods Blood cells of total of 77 cases with senile cataract and 76 controls were detected for GSTM1 gene, and the subcapsular epithelial cells of 22 cataract lenses were also detected for GSTM1 gene.

应用多聚酶链式反应（polymerase chain reaction， PCR）方法对77例老年性白内障患者及76例健康对照者外周血细胞进行GSTM1基因检测，并对其中的22例老年性白内障晶体上皮细胞进行GSTM1基因检测。

Results The GSTM1 gene deletion rate in cataract group was 53.25% and that in the control group was 46.05%, they being not significantly different statistically (χ2=0.750, P＞0.05, OR=0.75). GSTM1 gene deletion rate in the subcapsular epithelial cells of 20 cases was basically consistent with that in blood cells.

结果 白内障组GSTM1基因缺乏率为53.25%，对照组为46.05%，两组间差异无显著性(χ2=0.750， P＞0.05， OR=0.75)。20例老年性白内障晶体上皮细胞内GSTM1基因检测与外周血细胞检测结果一致(P＜0.01)。

XPD gene is regulated by its upstream regulatory gene P44, and its function requires the interaction of XPD with P44, which can result in the changes of cdk7, cdk2, cmyc and cdc25A genes expression. XPD/P44 subcomplex is involved in the regulation of DNA damage checkpoint.

XPD基因的表达受其分子伴侣P44的调节，XPD的作用需要有P44的参与；XPD/P44亚复合物可能是通过DNA损伤检控点来调控细胞周期的。

Methods Using recombinant adeno viruses,we expressed the B7.1 gene in murine breast tumor cell line EMF6 and a subline previously transfected with retrovirus vector XdF containing IL-2-TNFα fusion gene.

在小鼠乳腺癌细胞系EMF6和一个已被含有IL-2和TNFα融合基因的逆转录病毒载体XdF转染的EMF6亚系(T2-EMF6)中表达B7.1基因。

Transferred the NP-1 gene into C. ellipsoidea by electroporation with high efficiency expression vector pBinUΩNP1. PCR, Southern blot and Northern dot blot analysis of the G418resistant alga strains indicated the stable integration and correct transcription of NP-1 in C. ellipsoidea genome. In vitro antagonistic assays showed the proper expression of defense gene in C. ellipsoidea. Transgenic C. ellipsoidea is antagonistic to gram negative bacteria—Bacillus subtonic, gram positive bacteria—Escherichiacoli and fungi—Fusarium oxysporum to some extent.

以椭圆小球藻完整细胞为受体，采用电激转化法将连接在高效表达载体pBinUΩNP1上的兔防御素NP-1基因导入小球藻，通过PCR、Southern杂交、Northern点杂交对转基因小球藻进行了分子检测，同时通过体外离体抑菌实验检测了兔防御素NP-1基因表达产物的活性，结果表明NP-1基因已稳定整合到小球藻基因组中，并进行了正确转录和表达。

The DHFR gene of the sulfanilamide treatment group and positive control group were amplified 832bp.Compared the DHFR gene with the sequence gb[M26495](Pc f.sp.rat),the homology rates were both 99%.

实验组和阳性对照组Pc DHFR有832个碱基，与gb[M26495]序列比较，同源性均为99%，与gb[AF322061](Pc f.sp.rat)序列比较，同源性均为100%。

P51 gene is a new member of p53 gene superfamily.

P51基因是p53基因家族的一员。

Polymerase chain reaction amplification of a portion of a gene encoding a surface protein of Wolbachia was used to detect the Wolbachia infection of the population of Pteromalus puparum and Nasonia vitripennis by universal primers of wsp gene and the specific primers for wsp genes of A and B Wolbachia supergroup.

采用Wolbachia 的通用引物及A和B大组特异性引物对蝶蛹金小蜂Pteromalus puparum和丽蝇蛹集金小蜂Nasonia vitripennis体内Wolbachia的wsp基因进行PCR扩增及序列测定，并对测定的序列进行了同源性比较和基因特征分析。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。