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Methods mEBP gene was predicatively analyzed by DNASIS software, and the mutations at sites 5 and 18 were induced by PCR site-directed mutagenesis. The mEBP gene was cloned into prokaryotic vector pinpoint Xa-3 and transformed to E.

以亲代EBP基因为基础，结合DNASIS软件理化性质分析确定突变碱基，应用PCR定点诱变技术进行第5、18位谷氨酰胺→赖氨酸的定点突变。

Using TaKaRa LA PCR? in vitro Clonging Kit, a 2.3kb DNA fragment from the mutant was amplified and identified. Sequence analysis indicated that the fragment was composed of a complete proE gene and a partial proA gene.

根据枯草芽孢杆菌93151野生菌株proB基因的序列设计并合成引物，利用TaKaRa LA PCR~ in vitro Clonging Kit试剂盒，克隆突变菌株的proBA基因，得到一个约2.3kb的DNA片段，序列分析表明该片段包含一个完整的proB基因和不完整的proA基因。

These revealed that prolactin receptor gene could be used as a candidate gene for reproductive traits.

提示催乳素受体基因可作为繁殖性状的一个候选基因。

The promoter of rice seed storage prolamin 4a gene which was amplified in vitro using PCR from rice genome, linked to the β glucuronidase gene, was introduced into rice by particle bombardments.

利用PCR 方法从水稻"中花8 号"基因组中扩增出醇溶蛋白4a 基因启动子后，将其与GUS基因融合，通过基因枪法导入水稻"台北309"中。

These results intensively demonstrated that BMPR-IB gene is a major gene that affects the prolificacy in both Small Tail Han sheep and Hu sheep, and could be used as a molecular genetic marker to select for litter size in sheep.

BMPR-IB基因型在几个绵羊品种之间的分布以及各种基因型绵羊的实际产羔两方面的研究结果都证明BMPR-IB基因是影响小尾寒羊和湖羊高繁殖力的一个主效基因，可以用于对绵羊产羔数的辅助选择。

The frequency of genotype AA in prolific group and normal group were lower than low prolificacy group. It can be inferred that gene B made positive effect in prolificacy in Jizhong-goat, and gene A made negative effect in prolificacy.

高产群和普通群体的从基因型频率都比相应的低产群要低，可推测等位基因B对冀中山羊高繁殖性能有正向作用，而A基因对冀中山羊的繁殖性能有负向效应。

Methods : Using GV1 and GV2 targetable non-viral vectors, the influences of the poly-L-lysine and protamine on in vitro gene transfer efficiency and the course of gene expression were observed.

利用GV1，GV2靶向性非病毒载体来比较多聚赖氨酸与鱼精蛋白对靶向性基因转移复合体携带DNA的能力及体外基因转移效率的影响。

Little has been done on Bedlington coat colour, but a 'silvering' gene and a diluting gene are thought to be involved in the change from puppyhood to adult; whether 'black to blue' or 'chocolate to liver'.

贝的皮毛有很少的变化，但是一个'银色'的基因和一个颜色逐渐变浅的基因被认为在他们从幼年成长到成年中。

Objective To study the mutation of P53 gene an d ras gene in stomach cancer.

目的 研究胃癌组织中P53基因和ras 基因的突变情况。

The white gene is a recessive gene.

白的基因是一个退行的基因。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。