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In order to compare the homology of the gene encoding VP2 between MDPV-Q and MDPV which was registered in the GenBank, also in order to find out changes of the VP2 gene and the immuno-genicity between the wild-strain MDPV-Q and the attenuated strain MDPV-26 which derived by continued passage of virulent wild-type in muscovy duck embryo, a pair of primer (LHMP7/LHMP8) was designed. The upper one LHMP7 and the lower one LHMP8 corresponded to the MDPV-specific nucleotides sequence 2885-2900 and 4618-4604 respectively, according to the MDPV nucleotide sequence databases. The length of the sequence embraced by the primers was 1734bp.

为了获取这一基因片段与国外分离株进行同源性比较，同时也为了了解MDPV强、弱毒株VP2基因之间的异同关系，及其免疫原性的变化规律，通过DNA重组技术，设计了一对引物LHMP7／LHMP8，该对引物选取位于2885～2900及4618～4604的两段序列，跨幅为1734bp，并在这两条引物中分别加入两种限制性核酸内切酶SacⅡ和KpnⅠ的酶切位点，分别对MDPV-Q和由该株病毒经人工致弱后得到的MDPV-26株进行PCR扩增，并将PCR产物克隆到pMD18-T载体上，分别得到二个重组子：pMD18-T—M-Q VP2和pMD18-T-M-26 VP2。

HDAC inhibitor NaBu also promotes the expression of ALAS2 gene promoter reporter gene in a dose-dependent manner.

以启动子报告基因质粒转染细胞，然后用含有NaBu的培养基培养细胞，结果表明NaBu能以剂量依赖的方式增强ALAS2基因启动子转录活性。

We found that NaBu stimulated ALAS2 gene expression not only in erythroid K562 cells, but also in non-erythroid 293T cells with a concurrent increase in acetylation level of histone H3 and dimethylation level of K4 at the same histone at ALAS2 gene promoter.

同时ALAS2基因启动子的组蛋白H3乙酰化水平以及组蛋白H3的第4位赖氨酸的双甲基化水平升高。

Results There are 13 positive cases in 30 in situ Hybridization tissues of NPC, the p16 gene mRNA positive rate is 43.3%, and the p16 gene mRNA are positive in all of 20 cases of chronic Nasopharyngitis.

结果 30例NPC标本原位杂交阳性13例，p16 mRNA阳性表达为43.3%，3个高发家系的NPC标本无阳性表达，20例对照全部有p16 mRNA阳性表达。

Both scorpion neurotoxin gene and chitinase gene are valuable for producing transgenic crops.

蝎毒基因与昆虫几丁质酶基因都是重要的抗虫基因。

Dianne Nicol, a professor of law at the University of Tasmania, observes that this case turns on whether an isolated gene sequence has markedly different characteristics from a gene that occurs in the human body.

塔斯马尼亚大学的法学教授Dianne Nicol评论道：这个案子关注的是，一个被分离的基因序列是否与人身上的基因有明显不同的特性。

At last in this paper, the sequences of CHD-W gene in Chinese Egret, Cattle Egret and Black-faced Spoonbill,and the sequences of CHD-Z gene in Chinese Egret, Large Egret, Intermediate Egret, Cattle Egret, Night Heron and Black-faced Spoodbill were analyzed and used to study their relative and phylogenetic evolution .

本文最后对黄嘴白鹭、牛背鹭和黑脸琵鹭的W染色体上扩增的CHD片段，以及黄嘴白鹭、大白鹭、中白鹭、牛背鹭、夜鹭和黑脸琵鹭Z染色体上扩增的CHD片段进行序列分析并用于亲缘关系及系统进化的研究。

The carcinogenic mechanism of nitrosamine is that it may cause the changes of tumor related gene and suppressor gene in esophageal epithelisl cells.

其致癌机制研究显示，亚硝胺可引起食管上皮细胞相关癌基因抑癌基因发生改变，大大促进癌变。

Except for variation data, gene descriptions, enzyme information and other biological information for each gene locus are also included. Since RefSeq of NCBI http:∥www:. ncbi. nlm. nih. gov/LocusLink/refseq.

另外，VSD有别于现有的其他变异数据库的主要特点是：一是在基因组、mRNAs和蛋白质三个水平上定位了变异；二是分析了可能影响蛋白质功能的非同义单核苷酸多态性变异(nonsynonymous SNP，nsSNPs)。

The new chimeric infectious DNA clone and its derived, a virulent chimeric virus are constructed from the nonpathogenic PCV1 in which the immunogenic ORF gene of the pathogenic PCV2 replaces a gene of the nonpathogenic PCV1, preferably in the same position.

所述新型嵌合的传染性DNA克隆及其衍生的有毒力的嵌合病毒是用非致病性PCV1构建的，其中，致病性PCV2的免疫原性ORF基因取代了非致病性PCV1的基因，优选是在相同的位置上取代。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。