查询词典 Gene

Methods: In accordance with conventional reporter gene analysis reagent preparation method, the main component Luciferase, Luciferin and ATP were serial diluted to find the optimal concentration; Reaction solution was prepared according to the optimal concentration of the main component, then ATP were serial diluted to measure their reporter gene. Then standard curve of ATP were drawed. Tumor cell lines H1299 were serial diluted and their ATP were measured by reaction solution prepared to analyse the minimum cell number.

按照常规报告基因分析试剂配制方法，将其中主要成分Luciferase和Luciferin及ATP浓度进行倍比稀释后找到最适合反应的最佳浓度值；按照最佳浓度配制反应液，将ATP浓度倍比稀释测定报告基因数值并绘制标准曲线，分析检测灵敏度；另外选取肿瘤细胞株H1299，倍比稀释后裂解细胞应用配制好的反应液进行ATP检测，分析最低检测细胞数。

Firstly, the pGL3-Control vector was reconstructed , the pGL3-Enhancer vector deletious of SV40 promoter gene was obtained . Then the IFN-β promoter gene was cloned into the pGL3-Enhancer vector and pGL-IP21, the Luciferase reporter plasmid with IFN-β promoter was OK. The availability of pGL-IP21 was verified by NDV ,the inductor of IFN-β, the Luciferase activity was assayed in cells transfected with pGL-IP21 by Luminometer.

首先将pGL3-Control载体进行了改构，除去了SV40启动子基因，获得了pGL3-Enhancer载体，将获得的IFN-β启动子基因连入载体中，构建了带有人IFN-β启动子基因的荧光素酶报告质粒IP-21，并且通过实验证明所构建的质粒在干扰素的诱导剂NDV的作用下能够表达荧光素酶活性，照度计检测荧光素酶活性增强，从而验证了所构建的重组质粒的有效性。

The Cre gene was cloned from lysogen BM25.8 by PCR. The Cre gene was then inserted in between the CaMV35S-35S double promtor and Nos terminator of PBI525 plasmid after sequencing. The expression box was cut down and inserted into plasmid pBINPLUS to obtain the final fertility-restoring expression vector pBINPLUSCre.

PCR方法从溶原菌BM25.8基因组DNA扩增出Cre基因，克隆测序验证无误后插入PBI525 CaMV35S-35S双启动子和Nos终止子之间，再将表达盒切下插入pBINPLUS质粒相应位点，得到恢复基因植物表达载体pBINPLUSCre。

Objective: The objective is to observe the expression of HOXC4 gene、HOXC6 gene on the differentiation and proliferation of hematopoietic stem cell to colony Colony Forming Unit-T Lymphocyte in vitro. And the differentiation progress affected by HCMV and/or ATRA, and to explore the possible mechanism of HCMV-induced maldevelopment to human cord blood T-Lymphocyte progenitor in genic level.

目的探讨人类脐血造血干细胞向淋巴系祖细胞增殖过程中HOXC4、HOXC6基因表达的情况，并且用人类巨细胞病毒和/或全反式维甲酸进行干扰，在基因水平探讨HCMV感染导致脐血淋巴系祖细胞损伤的机制。

In the research, a lisianthus cultivar "Double Mariachi Pink"was used. The research included establishment of regeneration system of lisianthus,construction of chitinase gene in plant expression vector and transformation of lisianthus with chitinase gene.

本研究以洋桔梗"Double Mariachi Pink"品种为试材，建立了该品种的离体培养高频再生体系；构建了菜豆几丁质酶基因的植物表达载体，并对"Double Mariachi Pink"进行了转化。

In this article, to estimation approach of gene effect, we establish genetic model and develop an approach to estimate the polygenic effect and gene types, the validity of this approach is proved by mathematical induction and computer simulation.

针对上述两点，本研究分为两篇，上篇发展了一种估计控制数量性状的基因作用和类型的方法，并用数学推导的方法和计算机模拟的方法证明了所提方法的有效性。

This is not a gene for obesity, it is a gene that contributes to risk, Professor McCarthy said.

这不是导致肥胖的基因，它只是与这种风险有关。McCarthy教授认为。

The references for medullary thyroid carcinoma on gene changes,diagnosis and treatment with gene in recent years are reviewed.

本文对近年来甲状腺髓样癌基因改变，诊断和治疗研究近况进行了综述。

Phylogenetic analysis of the long terminal repeatand env gene of htlv-1 gave a phylogenetic classification of htlv-1 genotypes into five major molecular subtypes: cosmopolitan, japanese, west african, central african, and melanesian. in order to understand the major genotype of htlv-1 prevalent in china, htlv-1 env gene was amplified by pcr and sequenced from peripheral blood lymphocyts of three htlv-1 carriers in the putian region of fujian province.

根据基因组及血清学反应可将其分为人t淋巴细胞白血病病毒1型（htlv-1）和2型（htlv-2），两型病毒基因组同源性大于60％。htlv-1主要侵染t淋巴细胞，可引起成人t细胞白血病、热带下肢痉挛性瘫痪或htlv-1相关脊髓病，并且在多种自身免疫病患者体内也发现了该病毒的存在。

RNA interference, as an important gene silencing methods, has a significant application to the research of gene function and genetic melioration.

RNAi干涉是一种重要的基因沉默方式，它在基因功能和遗传改良研究中有重要的应用。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。