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The thymidine kinase gene of infectious laryngotracheitis virus Beijing E2 strain was amplified by PCR, cloned into pGEM-T vector, and sequenced. Sequence analyses showed that the TK gene was very closed among sequenced ILTV strains.

以纯化的传染性喉气管炎病毒北京E2株基因组DNA为模板，通过PCR方法扩增了ILTV北京E2株的完整TK基因，将其克隆到pGEM-T载体上，并测定了序列，与其它ILTV毒株TK基因比较说明，ILTV不同毒株的TK基因高度保守。

This stuty consists of five parts: In the first part, amastin gene from DNA of two Leishmania donovani isolateswas amplified respectively by PCR. Amastin gene then was cloned into prokaryoticexpression plasmid pET-32a.

本研究共分为五部分：第一部分，以两株杜氏利什曼原虫基因组DNA为模板，通过PCR扩增获得了与预期大小一致、约552bp的杜氏利什曼原虫amastin基因。

Bacillus thuringiensis; cry gene; gene cloning; Lepidopteran pest

苏云金芽孢杆菌； cry基因；基因克隆；鳞翅目害虫

Gene diversity (of all the gene diversity of populations) between populations accounted for 24.34%; Which showed that Lespedeza bicolor had abundant genetic diversity of populations and great genetic potential on breeding.

种群内的基因多样性占总群体的75.66%，种群间占24.34%，表明二色胡枝子种群具有丰富的遗传多样性，在育种上具有很大的遗传潜力。

Objective: To explore the relationship between various mitochondrial DNA tRNA Leu gene/ND-1 gene mutations and type 2 diabetes. Methods: Case-control study was designed.

目的：探讨DNA tRNA Leu基因及ND-l基因3316G/A、3394T/C等4个突变位点与2型糖尿病的关系。

The effect and variation of major gene are estimated by the maximum likelihood method implemented via expectation-maximization algorithm. Major gene is tested with the likelihood ratio test statistic.

该方法以EM算法实现的极大似然估计方法进行主基因的效应估计，以似然比统计量进行主基因的各种遗传假设检验。

Result The fiber color was controlled by 4 pairs of genes at least. Whether the long fiber and linter were brown was controlled by a pair of dominant genes separately, the type of its fiber color was controlled by 2 pairs of minor genes at least, showing different types such as light brown and brown approaching white. The long fiber in F2 generation had 3 phenotypes altogether of brown long fiber and brown linter, white long fiber and white linter, white long fiber and brown linter, showing there was interaction among genes and the expression of dominant gene in brown long fiber inhibited the expression of recessive gene of linter color.

结果］纤维色泽至少受4对基因控制，长纤维和短绒棕色的有无各由1对显性基因控制，其纤维色泽类型至少还受2对微效基因控制，表现出淡棕、棕近白等不同类型。F2代长纤维共有棕色长纤维棕色短绒、白色长纤维白色短绒、白色长纤维棕色短绒3种表现型，表明基因间存在互作，棕色长纤维显性基因的表达抑制短绒色泽隐性基因的表达。

As the gene transfer ,liposome has mainly been applied in research on gene transfection and research on function of genome.

脂质体作为基因载体主要被应用于基因转染和基因组功能研究，并取得了较大的成绩。

In this study, we constructed a fast screening system with target gene and luciferase reporter gene.

用载体表达siRNA的方法并通过RNA干扰达到抑制乙型肝炎病毒的复制及其基因的表达。

Objective To establish dual luciferase report gene system basic the Y-box sequence of MDR1 gene and analysis it s activity.

目的 通过构建以MDR1启动子为启动序列的双荧光素酶报告基因系统并进行活性分析，为MDR1基因表达的单靶点调控研究和逆转剂的筛选提供一种有效的方法。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。