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In this study,two SNPs of chicken FASN gene were chosen to analyze genetic diversity and their associations with chicken growth and carcass traits by using a F_2 designed resource population.Results showed that:(1) In the PCR-RFLP site of HaeⅢ,allelic frequencies differed much among different chicken breeds,as B frequencies are the highest in Red Jungle Fowl(0.85),and the lowest in Taihe and Yugan Black(both 0.21).In the PCR-RFLP site of PstⅠ,A frequencies are the highest in Chongren Partridge(0.5),and the lowest in Naked Neck chicken(0.19),and the difference of gene frequencies was small among chicken breeds.

研究了脂肪酸合成酶(Fatty acid synthase， FASN )基因最后1个内含子2个突变的多样性，并运用资源参考家系研究了这2个突变对鸡体重、脂肪沉积性状的影响，结果表明：(1)红色原鸡Hae Ⅲ-PCR-RFLP位点B基因频率为最高(0.85)，泰和鸡及余干乌黑鸡为最低(0.21)，品种间差异较大，但未发现肉用与蛋用鸡种间的差异；在Pst I-PCR-RFLP位点上， A基因频率在崇仁麻鸡中表现为最高(0.5)，在裸颈鸡为最低(0.19)，品种间差异较小。

The lack of liver and kidney type PBMC in patients with alopecia areata Thl/Th2 type cytokine gene expression imbalance, presented to Th1-type cytokines and transcription factor T-bet gene over-expression of Th1-type response characteristics, may be involved in the pathogenesis of alopecia areata, alopecia areata may be insufficient evidence of liver and kidney-shaped one of the micro-nature.

肝肾不足型斑秃患者PBMC中Thl/Th2型细胞因子基因表达失衡，呈现以Th1型细胞因子和转录因子T-bet基因过度表达为特征的Th1型反应，可能参与了斑秃的发病机制，也可能是斑秃肝肾不足证型的微观本质之一。

Gene knock out is one of the most effective methods to study the gene function.

基因敲除技术是目前研究特定基因功能最为直接有效的实验手段。

Abstract〗 Objective To investigate the effect of gene gun transduction of Kras antisense gene on cell growth in human pancreatic carcinoma.

摘要〗 目的探讨基因枪转导Kras突变反义基因对胰腺癌细胞生长的影响。

Objective To discuss the relationship between Kras gene activation and MTS1/p16 gene mutation/deletion and the progression of endometrial carcinoma.

目的 探讨Kras激活与MTS1/p16基因突变/缺失在子宫内膜癌中的发生发展关系。

Gene gun transduction of Kras antisense gene into carcinoma cell can significantly inhibit cell growth of Kras mutant pancreatic carcinoma cell lines.

转导Kras突变特异性反义基因后，具有突变型Kras基因的胰腺癌细胞系的生长都受到明显抑制。

It means that decreased expression of Adipo R2, Krs, TNF-αmight be relateted to the action of lipid metabolism by EA treatment, and the down-regulation of Utr gene expression was might be involved in cell damage after simvastatin treatment. To confirm the effect of simvastatin on the changes of gene expression and the state of cell growth, C2C12 was treated in 0, 1, 3, 10, 30 and 100μmol/L of simvastatin.

在正常组、高脂组、辛伐他汀组、针治组、针预组、针非组6个组中，AdipoR2和Krs在针治组表达量最低，TNF-α在针非组中最低，Utr在他汀组中最低，与差异基因的筛选结果一致；同时也说明AdipoR2、Krs、TNF-α在针刺组中下调表达可能与其降脂作用有关；而Utr基因在他汀组中表达减少，可能与该药对肝细胞的损伤作用有关。

In this paper,we directly cloned 2.366Kb cDNA sequence of human lactoferrin gene and 800bp 5′flank regulatory sequence of β/lactoglobulin gene from goat by PCR,then connected them with the expression vector pLNCX.

通过PCR法直接克隆了2.366kb的人乳铁蛋白基因cDNA序列及800bp的山羊β乳球蛋白基因5′端调控序列，并连接到表达载体pLNCX中。

Fig 1 Negative control Female umbilical cord sample has no Y-specific signal after in situ hybridization with the biotinylated Y-repeated sequence DNA probe PY3.4 Fig 2 Positive control Flow-sorted male umbilical cord cells hybridized to Y-specific DNA probe PY3.4,Every cell contains a Y-specific signal Fig 3 Fetal cells sorted from maternal blood Flow-sorted cells from a pregnant woman at 20 weeks of gestation hybridized to Y-specific DNA probe PY3.4,containing a Y-specific signal Fig 4 The result of polymerase chain reaction Lane 1:male umbilical cord NRBCs sorted by FITC-conjugated anti-monoclonal glycophorin A;Lane 2:sample of 2;Lane 3:male umbilical cord NRBCs sorted by FITC-conjugated anti-CD36;Lanes 4-5:samples of 1 and 3;Lane 6:50 cells of male;Lane 7:5 cells of male;Lane 8:200ng male DNA(positive+control);Lane 9:nonpregnant female DNA(negative+control);Lane 10:ΦX174 HaeⅢ Maker,271bp:amplified band of Y-specific gene SRY;383bp:amplified band of human β-globin gene

图1 阴性对照女性脐带血标本，经与生物素标记的Y-染色体重复序列DNA探针原位杂交后未见Y-染色体特异信号图2 阳性对照分选的男性脐血细胞经与Y-特异DNA探针杂交后，每一细胞都含有Y-染色体特异信号图3 母体外周血中分选的胎儿细胞从一位妊娠20周的孕妇外周血中分选出的细胞经与Y-特异DNA探针杂交细胞中含有Y-染色体特异信号图4 聚合酶链反应结果 1：GPA-FITC单抗分选男胎脐血NRBCs；2：2号标本；3：CD36-FITC单抗分选男胎脐血NRBCs；4、5：1、3号标本；6：50个男性细胞标本；7：5个男性细胞标本；8：200ng男性DNA；9：未孕女性DNA；10：ΦX174 HaeⅢ标准，271bp：为SRY基因扩增带；383bp：为人β-珠蛋白基因扩增带内参照

The clusters of orthologous groups involved in a gene network usually are large groups and therefore LAPP is also an approach for complex gene logic networks.

由于涉及功能的基因组通常是一个大的群体构成的系统，因此LAPP方法也是一个生成复杂的基因逻辑网络的方法。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。