查询词典 Gene

This may lead to a change of certain gene components, which is prerequisite for an incisive reorganization of the gene

这又会引起某些基因成分的改变，基因成分的改变是基因成分彻底改组的前提。

Protein is the production of gene expression and the final indicator of the function of gene, so proteomics has become one of the main contents of functional genome.

蛋白质作为基因的表达产物、基因功能的最终体现者，蛋白质组学已成为功能基因组学的主要研究内容之一。

To construct an stx2 gene mutant phage FMin27 and to observe its infectiousness of various serotypes Escherichia coli strains. With the help of Red recombinant system, the stx2 gene of the E.

通过构建一株stx2基因突变的志贺毒素2型噬菌体，来观察它对不同血清型大肠杆菌的感染特性。

The simulating results show that this new kind of gene gun not only meet the speed requirement of practical gene gun application,but also has an easy speed controlling characteristics by means of the initial charge voltage of the driving capacitor in the driving system.

仿真结果表明，新型基因枪的微弹发射速度不仅可以通过调节其驱动电路的初始充电电压的幅值来控制，而且其微弹发射速度能够满足基因枪的实际使用速度要求。

The result showed that twocDNA fragments which expressed at high level both in shoot and radicle representedthe gene encoding beta-D-glucosidase; one cDNA fragment expressed specifically inshoot represented the gene encoding mitochondria HSP60; most clones of MF12 andMF17 fragments respectively represented the chloroplast genes encoding prp22 andprp19 proteins which are two components of ribosomal small subunit; while thededuced amino acid sequence from each exceptional one clone was respectivelyhomologous to CDC5 proteins and vesicle-associated membrane proteins; otherthree cDNA fragments expressed preferentially in shoot had no homologue inGenBank.

结果发现2个在胚芽和胚根中表达量都很高的cDNA片段代表的是编码玉米β-D-葡糖苷酶的基因；一个在胚芽中表达而在胚根中不表达的片段代表的是编码线粒体分子伴侣HSP60的基因；片段MFl2的大部分克隆测序结果是与叶绿体基因组中编码核糖体小亚基蛋白prp22的基因同源，但其中有一个克隆测定的cDNA片段序列推测的氨基酸序列与CDC5家族成员有较高的同源性；片段MF17的大部分克隆测序结果与叶绿体基因组中编码核糖体蛋白prp19的基因同源，而有一克隆测定的cDNA片段序列推测的氨基酸序列与参与信号转导的膜结合蛋白VAP27和VAMP有较高的同源性；另有3个优先在胚芽中表达的cDNA片段未查询到同源序列。

It is impossible to obtain cells, however, in which the target gene was persistently inhibited with siRNA, Therefore it is potential to use plasmid-based RNA interference technology to study gene function.

但是由于siRNA的不稳定性，采用siRNA研究基因功能不能得到抑制靶基因表达的稳定细胞克隆，因此以质粒为基础的RNA干扰技术表现出了它的应用潜力。

Finally, We searched for paralogous genes appeared in the contrary differentially pathways of normal and disease status, respectively. It is very likely to find an intersectional gene between different pathways, because this intersectional gene has higher probability to associate with both these paralogous genes in different situation.

最后搜寻疾病和正常的路径之间的paralogous基因，也许可能会在两组之间找到相同的基因，而这相同的基因可能和paralogous基因有关。

Strains M110 and M113 isolated from Aedes albopictus and Armigeres durhami, respectively, which were fatal to suckling mice at 4 days after intracerebrally inoculation and had cytopathologic effects on BHK21 cells, showed a positive reaction to dengue antibody and could be amplified Ly using flavivirus primers and dengue type-4 NS1 gene primers and dengue type-4 NS2a gene primers.

结果 从白纹伊蚊和达勒姆阿蚊中各分离到一株病毒，分别命名为M110和M113，这两株病毒对BHK21细胞能产生明显的细胞病变，脑内接种乳鼠4d左右导致乳鼠死亡。该病毒经血凝、血凝抑制和间接免疫荧光试验提示为黄病毒。分别用黄病毒属特异引物、登革4型病毒NS1和NS2a基因片段特异引物进行RT-PCR扩增、序列测定，证实为登革4型病毒。

These variations may have some effect on gene structures and gene expression, and then contribute to intraspecific phenotypic diversities.

在物理图提供的序列基础上，对于籼粳稻两个主要栽培稻亚种进行了比较基因组分析，在2.3Mb对应区段中发现了两个亚种在基因组成和顺序上存在的广泛的微共线性，两者之间的差异主要由插入/缺失和单核苷酸多态性造成，这些差异可能影响基因的结构和表达，从而对种间表型差异产生影响。

Methods The IPSE gene of S. man soni was synthesized by overlapping PCR, and confirmed by DNA sequencing. The recombinant plasmid IPSE-pET32a was constructed by inserting the gene of IPSE into expression vector pET32a at the downstream of thioredoxin coding sequence.

应用重叠PCR方法合成曼氏血吸虫IPSE基因，将其定向克隆至表达载体pET32a的硫氧还蛋白序列下游，构建重组表达质粒IPSE/pET32a。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。