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Along with the fluconazole solution density rise,the experimental two kind of strain various glucose density is higher,showe d the glucose consumption are less,takes the logarithmof the medicine de nsity,discovered the logarithm the medicine density and each glucose den sity presents the linear relations;Carries on the analysis comparison to under the fluconazole function two kind of strain linear relations,disc overed the relations of the two strains has the nonuniformity.3 Compare the fluconazole induction reaiatance SC5314 strain and sens itive strain compares,its difference gene expression mainly concentrates in:The code proteinase body and the protein hydroltyic enzyme gene,in the code sugar fat metabolism process is connected the protein gene,the cell cycle correlation gene,the duplication and the translation adjustme nt correlation gene,the stress response correlation gene,the line plast ochondria correlation gene,the cell wall function related gene.4 Candida albicans SC5314 induction resiatance strain was processed b y Xianglian solution,its expression change gene mainly is:Code stress re sponse family protein gene,biomembrane relevant gene,a code proteinase body gene race,code cell cycle related protein gene,duplication and tra nslation adjustment related protein gene.5 The clinical reaiatance strain Candida albicans was processed by Xi anglian solution,its expression change gene mainly is:Codes the hot sho ck protein gene,the serine/threonine protein activating enzyme gene,the proteinase body family gene,the regulation copies and translates the ge ne.

随着氟康唑药液的浓度上升，试验的两种菌株各孔葡萄糖浓度越高，说明葡萄糖消耗越少，经过药物浓度取对数后进行分析，发现取对数后的药物浓度和每孔中葡萄糖浓度者呈现线性关系；对氟康唑作用下的两种菌株的线性关系进行分析比较，发现对两种菌株作用具有不一致性。3氟康唑诱导的耐药SC5314菌株与诱导前的敏感株相比，其差异基因表达主要集中在：编码蛋白酶体及蛋白水解酶的基因，编码糖脂代谢过程中相关蛋白的基因，细胞周期相关基因，转录及翻译调节相关基因，应激反应相关基因，线粒体相关基因，细胞壁功能相关基因。4白念珠菌SC5314诱导耐药株经香莲外洗液作用后，其表达变化的基因主要是：编码应激反应家族蛋白的基因，生物膜相关性基因，编码蛋白酶体基因一族，编码细胞周期相关蛋白基因，转录及翻译调节的相关蛋白基因。5白念珠菌临床耐药菌株经香莲外洗液作用后，其表达变化的基因主要是：编码热休克蛋白基因，丝氨酸/苏氨酸蛋白激酶基因，蛋白酶体家族基因，调控转录及翻译基因。

To study the applicable prospect of suicide gene on tumor therapy in clinic,we cloned the gene of D-amino acid oxidase and the gene of yeast cytosine deaminase.We hope to establish the transgenic mices of DAAO gene and YCD gene,and the two transgenic strain mices are valuable animal system for studying the biological characteristic of DAAO gene and YCD gene ,for studing the killing activity of DAAO/D-Ala and YCD/5-FC gene therapy systems on tumor.The D-amino acid oxidase gene derived from R.gracilis and it could oxidize D-amino acids. Hydrogen peroxide(H2O2) is a reactive oxygen species generated in the deamination of D-Ala catalyzed by DAAO.

为了探讨自杀基因在临床肿瘤治疗中的应用前景，建立自杀基因肿瘤治疗评价的动物模型，我们克隆了D-氨基酸氧化酶(D-amino acid oxidase，DAAO)基因和酵母菌胞嘧啶脱氨酶(yeast cytosine deaminase，YCD)基因，希望建立DAAO基因及YCD基因转基因小鼠，为研究DAAO及YCD基因的生物学特性、开发和评价DAAO／D-Ala及YCD／5-FC自杀基因系统进行肿瘤治疗建立良好的实验动物模型。

After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象，提取细菌基因组DNA，用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切，回收大小为1～5Kb的DNA片段，将其连接入原核表达载体pET-28a-c，最后转化宿主菌，结果成功地构建了基因表达文库，为后续的基因筛选工作奠定基础；另外，本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计，以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板，进行PCR扩增反应，结果表明成功地获得两个新基因：23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸)，并进一步做了不同物种之间的分子系统发育分析，构建了基于23S rRNA基因的邻接法系统发育树，阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

There are 11 gene bindings disappear in the normal group, but appear in the model group, and then disappear in the treat group, that indicates the 11 gene bindings are correlated with the epilepsy and Caohuozhimutang. After searching in the Gene Bank, 7 bindings in the 11 gene bindings are homogenic with the 8q31 ribosomal protein gene, 15p12 paraneoplastic neuronal antigen gene, 4q22 diacylglycerol kinase iota gene, xq37 FMR2 protein and 11p11 roudabout homolog 1, and 4 bindings of which are novel gene.

经NCBI美国国家基因库检索，这11条基因中的7条已知基因分别与位于鼠染色体8q31位置的核糖体蛋白、15p12位置的周围肿瘤神经元的抗原(paraneoplastic neuronal antigen)、4p22位置的甘油二酯酶、xq37位置的FMR2蛋白（FMR2 protein）的基因以及以及位于果蝇染色体11p11位置的交叉同族体（roundabout homolog 1）有极高的同源性；有4条基因片段同源性较低，为功能未知的新基因，已经在GeneBank进行了注册，注册号分别CK325391、CK325392、CK325393、CK325394。

Gene sequence was highly homologic with a gene encoding aβ- subunit of ATP synthetase and an EST originating from a cDNA in relation to water deficit stress, which both were closely associated with the protein functioning in stress resistance and ion transportation. The results suggested that No. 1 gene sequence was involved in stress resistance of apple rootstock. No. 2 gene sequence had high homology with a gene encoding a serine/ threonine kinase protein, indicating perhaps it was a new gene encoding the serine/ threonine kinase protein in apple rootstock. The gene sequences of A1 and A2 were highly homologic with a gene encoding aβsubunit of ATP synthetase.

对得到的差异片断进行回收、测序，通过BLAST和其它植物比对，发现Ⅰ号序列与一条编码ATP合酶β亚基基因和一条水分亏缺胁迫cDNA克隆基因EST的同源性非常高，该序列编码涉及到了抗性表达、离子转运基因，可能参与了植物抗性表达，说明获得的基因序列应是与苹果砧木抗性相关的基因；Ⅱ序列与一条serine/threonine kinase蛋白激酶的蛋白序列同源性比较高，核酸比对较低，可能是具有丝氨酸/苏氨酸蛋白激酶功能的新基因。A1、A2序列与一条编码ATP合酶β亚基基因的同源性较高。

Moreover, CoI gene was more conservative and a lower evolutionary rate than Cyt b gene. CoI gene was an effective marker in analysing the phylogenesis of families in Passeriformes. It can be used in identifying the species of Passeriformes, but it was revealed that CoI gene was more suitable to identify the phylogenetic relationship of avian family unit than Cyt b gene, and CoI gene can be a molecular marker to identify avian species. But in species identification, CoI gene was less stable and accurate than Cyt b gene. We suggested youd better employ the other marker if you have the CoI gene only.(2) In the phylogenetic trees of birds from Lanius, L.

比较分析了雀形目6科15种鸟类的细胞色素b全序列和CoI基因部分序列，结果显示细胞色素b和CoI基因序列的变异位点分别为454个和366个、简约信息位点为337个和303个，而且线粒体CoI基因比细胞色素b基因略微保守、进化速率也较低；CoI基因在确定雀形目科级阶元之间的系统发生关系方面是一种有效的分子标记，同时它也能够用于雀形目鸟类的物种鉴定，但在物种鉴别方面不如细胞色素b基因稳定、准确。

On the bases of two parts above, in chapter 3, the ethical issues about the HGP are analyzed:①gene privacy invades human beings' autonomies;② gene discrimination breach human beings' dignity;③gene eugenics will result in human's lose;④"gene determinism" may result in human right's damage;⑤ gene patent can not be defended morally;⑥gene therapy may invade sufferers' autonomies;⑦issues about gene crime and gene weapon.

在具备了前两部分的基础上，在本文的第三部分对人类基因组计划进行了价值伦理分析：①基因隐私侵扰人的自主权；②基因歧视违背了人的尊严；③基因优生将导致人性的丧失；④&基因决定论&将导致对人类权利的侵犯；⑤基因专利在道义上是不能辩护的；⑥基因治疗可能违背患者的自主权；⑦基因犯罪和基因武器问题。

Therefore it is interesting to clone genes related to lemma and palea. In this thesis, six genes from the lemma/palea related gene pool which is established by Liu (2003) were further studied. Those genes are salT gene (salt-induced protein), GA-SPY gene (gibberellin action negative regulator SPY-related protein), U2AF gene (U2 snRNP auxiliary factor, small subunit-related protein), kinesin-like gene, DnaJ-like gene, and EF hand gene. At first, the gene expression in leaves and 1~4 cm inflorescences of normal lemma/palea, smaller lemma/palea, and stunted lemma/palea was compared by Real Time RT-PCR.

从刘(2003)所建立水稻颖花发育相关候选基因库中，挑选6个基因：salT基因(salt-induced protein)、GA-SPY基因(gibberellin action negative regulator SPY-related protein)、U2AF基因(U2 snRNP auxiliary factor，small subunit-related protein)、kinesin-like基因、DnaJ-like基因、及EF hand基因，以水稻正常型颖花、小颖花突变体及内外颖退化突变体之叶片及1~4公分幼花序为材料，利用Real-Time RT-PCR分析，发现salT基因在突变体幼花序中大量表现。

If these warriors performed well, they would be rewarded by having their genes added to the gene pool.

如果这些人表现很好，他们的基因将被加入gene pool作为奖励。

Ben is now 75 and, like Gene Abegg, 81, at Illinois National and Louie Vincenti, 73, at Wesco, continues daily to bring an almost passionately proprietary attitude to the business. This group of top managers must appear to an outsider to be an overreaction on our part to an OEO bulletin on age discrimination. While unorthodox, these relationships have been exceptionally rewarding, both financially and personally. It is a real pleasure to work with managers who enjoy coming to work each morning and, once there, instinctively and unerringly think like owners. We are associated with some of the very best.

Ben今年75岁，但与伊利诺国家银行81岁的Gene Abegg以及Wesco 73岁的Louie Vincenti一样，每天依旧为所领导的企业灌注无比的热情与活力，外界不知情的人还以为我们对于这群杰出的经理人有年龄上的特殊偏好，虽然极不寻常，但这样的关系实在是让我们受益良多，无论是在财务上或精神上都是如此，与这群&乐在其中&并以像老板一样心态每天认真经营公司的专业经理人在一起工作实在是一种享受。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。