查询词典 Gene

Gene orders of p53 gene （exon 1-11） from all of 10 samples were tested.

检测10份样本中卢53基因外显子1—11的基因序列。

Gene mutations in p53 gene exon 11 were identified in all samples, which had the same type and site.

p53基因外显子11在所有的被检测样本中均发现突变，而且突变的位点及突变的类型均相同。

Sequence encoding Pseudomonas exotoxin A translocating peptide was localized between antibody gene and GrBa gene.

表达的immunoGrB蛋白进入肿瘤靶细胞的内吞小体后，发生Arg279和Gly280之间肽键的定点裂解，裂解后的PE Ⅱ-GrBa被转位到细胞质中。

According to experimental results and etiopathogenisis and pathology of osteoporosis , and analyzing review investigation on hepcidin、associations of bone metabolism and iron metabolism and relations of ferri ion and calcium ion,we can make conclusions as follows: 1、We applied single manmade intervention approach to induce osteoporosis model in this study, there was no other influential factor. In the termination of test , rats bone density and bone histological anatomy indicated that osteoporosis model was creditable,meanwhile hepatic functional enzyme and hepatic microtome section demonstrated that hepatic function had noci-influence induced by medicinal herbs resource. Accordingly, we could believe that hepatic hepcidin gene expression and serum hepcidin contents can represent internal iron metabolism changes at selected test time. 2、In this investigation, different findings between test group and control group indicated that:according to changes of hepcidin gene expression measured by RT-PCR and changes of serum hepcidin contents determined by ELISA kit , there were correlations between hepcidin and rat osteoporosis model induced by retinoic acid. 3、Under the condition of this investigation,there was a interactive hypothesis as follows: Hepcidin iron metabolism←→ferriion←→calcium ion←→osteoporosis.

根据本次实验结果，对骨质疏松的病因病理的认识及铁代谢与铁调素、骨代谢与铁代谢关系、铁离子与钙离子的关系系列研究的回顾，经分析得出如下结论：1、本研究骨质疏松模型采用单一人工干预方法，没有其他影响因素；实验结束时大鼠骨密度、骨组织学检查表明骨质疏松形成，同时肝脏功能酶和HE切片显示肝脏功能未受药源性产生明显损伤性影响；因此，同期不同时间组大鼠肝脏铁调素基因表达、血清铁调素含量可以代表体内铁代谢不同时间的变化。2、在本研究中，模型组与对照组研究结果的差异表明：铁调素基因RT-PCR变化、血清铁调素ELISA测定含量变化与维甲酸制作的大鼠骨质疏松模型形成有实验相关性。3、在本研究平台下，形成了一个互相影响的假设图：铁调素←→铁代谢←→铁离子←→钙离子骨质疏松。

I studied all sorts of my gene message, for example, I am informed me to have gene of fast flesh fibrous.

我研究了我的各种基因讯息，例如，我得知我有快速肌纤维的基因。

After Finny' dead, Gene joined army, but Gene could not get away from this shadow in the next 15 years.

在菲尼逝后吉恩加入了军队，可是在十五年的时间里他都未能从此阴影中逃脱。

Gene was jealous of Finny's achievements in secret while he thought Finny envied himself. Unconsciously, Gene did the thing that made him regret in all his life.

由于暗中嫉妒菲尼的成就又怀疑菲尼对自己心怀嫉妒，吉恩在不自觉中作下了令自己后悔终身的事。

METHODS: After AIF△1-120 gene was successfully cloned, the shorter truncated human AIF gene (AIF△1-400) was constructed by deleting the Nterminal mitochondrial localization sequence, the coding sequence of flavine adenine dinucleotide binding domain and nicotinamide adenine dinucleotide binding domain of AIF protein.

在AIF△1-120基因克隆成功的基础上进一步改造，构建截去AIF基因线粒体定位信号，FAD结合结构域和NADH结合结构域（1-400位氨基酸）编码序列的AIF△1-400基因，将其克隆入pcDNA3和pIRES2EGFP真核表达载体，用脂质体法转染HeLa细胞，通过荧光显微镜观察，MTT检测等方法检测该基因在转染细胞中的表达及其对肿瘤细胞的促凋亡活性。

The study cloned the flic gene of App and performed sequencing and analysis of biological information. Polymerase chain reaction PCR was used to amplify the flic gene from.

构建猪传染性胸膜肺炎放线杆菌(Actionobacillus pleuropneumoniae， App)鞭毛蛋白编码基因的重组表达质粒，对其编码蛋白进行生物信息学分析，并将其在原核细胞中进行表达。

The flic gene of App was obtained by PCR based on highly homology with Escherichia coli, Salmonella, Shigellosis. Then it was legated to pMD-18T vector. The recombinant plasmid was identified by enzyme and sequence analysis. The 528 bp fragment of flic gene was successfully cloned into pGEX-KG vector, then the recombinant plasmid was transformed into E.

以猪传染性胸膜肺炎放线杆菌血清Ⅰ型菌株基因组为模板，根据其鞭毛区与其它细菌高度同源性设计引物，利用PCR方法扩增鞭毛蛋白基因flic片段，将其亚克隆到pMD-18T载体中并进行PCR和酶切鉴定，再将其亚克隆与载体pGEx-kG分别双酶切和连接，构建重组表达载体pGEX-flic，并将其转化大肠杆菌工程菌BL21，进行原核表达。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。