查询词典 Gene

Objective To clone the EG95 gene of Echinococcus granulosus in Inner Mongolia and analyze the gene nucleotide sequence.

作者：李志伟作者单位：内蒙古大学生命科学学院，呼和浩特 010021

The genetic analysis indicates that the new ellipsoid egg is controlled by a recessive gene which is independent of gene dp.

遗传分析结果：新的长形卵是由与dp独立遗传的隐性基因支配。

The expression level of tim gene in 25LL is markedless higher than 15DD and 20LD in the early incubation stage(7 days before), but it is obviously higher than 20LD and 15DD conditions in the late.To further invesgate the per and tim expression in different moment every day of the sensitive period(7d,8d,9d) during the process of embryogeny,the expression of tim gene was very low and the per and tim expression did not have obvious rhythmicity under 15DD condition.

进一步调查家蚕胚胎对环境敏感时期(7d-9d)per和tim基因的昼夜表达节律，在15DD条件下，tim基因表达量一直很低，tim基因和per基因的表达量都没有明显的节律性变化；而25LL和20LD条件下，两基因在一日中不同时刻的表达具有明显的节律性，表达量也较高。

A set of spelt monosomic lines as female parent were respectively crossed with wheat new variety SM96 and new line SM20005 to determine the chromosomal location of the gene conferring resistance to powdery mildew in both. Seeding of parents, F〓 and F〓 from monosomic F〓 were inoculated with race no. 15 of Blumeria graminis f. sp. tritici. Analysis of obtained data revealed that one major dominant resistant gene originating from wild Emmer in the variety SM96 and the line SM20005 was located on chromosome 4A.

为确定小麦新品种沈免96和新品系沈免20005含有的抗白粉病基因所在染色体，以一套斯卑尔脱单体系为母本分别与沈免96和沈免20005杂交，将亲本、F〓、单体F〓植株的F〓代群体在苗期用小麦白粉菌15号小种的单孢菌系接种鉴定，单体分析结果表明，沈免96及沈免20005所含源于野生二粒小麦的抗小麦白粉菌15号小种的1个显性主效基因位于4A染色体上。

Objective To observe the effect of Shenghui Keli on AD encephalon neural degeneration induced by A β(β-Amyloid protein) from neural function, biochemical and gene expression. Research the degeneration related to apoptosis of cell and gene expression correlate to apoptosis. Then investigate the mechanisms of Shenghui keli treating AD .

目的 从神经功能形态、生化与基因表达等方面，观察生慧颗粒对β-淀粉样蛋白(β-Amyloid protein，Aβ)诱导脑神经细胞变性的干预作用，以及与细胞凋亡、凋亡相关基因表达的关系，探讨生慧颗粒治疗Alzheimer病的作用机制。

METHODS: Tum5 gene was amplified from plasmid pSPORT1Sfi by PCR technique and subcloned into the expression plasmid of lentiviral vector, pGCFU, to generate the lentiviral expression vector, pGCFUTum5. The correct Tum5 gene was confirmed by endoenzyme digestion and sequencing. Recombinant lentiviruses were produced by 293T cells following the cotransfection of pGCFU Tum5 and packaging plasmids-pHelper1.0and pHelper2.0. The resulting recombinant lentiviruses (GCFUTum5) which carried Tum5 and EGFPgene were then used to infect human umbilical vein endothelial cells.

采用PCR技术从含有tumstatin基因的质粒克隆模板 pSPORT1Sfi钓取Tum5基因，并将基因克隆到慢病毒载体表达质粒pGCFU中，构建慢病毒载体表达质粒pGCFUTum5，通过酶切、测序验证Tum5基因后，将pGCFUTum5质粒和包装质粒pHelper1.0，pHelper2.0共同转染人胚胎肾上皮细胞系293T细胞，获得携带Tum5基因和EGFP基因的重组慢病毒GCFUTum5，并转染靶细胞人脐静脉血管内皮细胞。

Result The expression of eNOS gene promoted liver regeneration while the expression of IL-10 gene inhibited it.

结果 eNOS的表达同肝细胞的再生呈正相关，而IL-10的表达与其呈负相关。

We also inserted an Enterokinase recognition site between the hSOD gene and the small peptide gene, then we can cleave away the small peptide from fusion protein easily.

在构建时还在小分子多肽与人SOD的连接区插入肠激酶识别位点，这样可以使小分子多肽能从融合蛋白中切割下来。

III For constructing the expression vector of a fusion protein and obtain a target protein with full identity on aa sequence of a natural 13- 1,3-1 ,4-glucanase, with the recombined plasmid DNA harbouring the target gene as template, the primers designed with restriction sites for both terminals and enterokinase recognition site, followed by PCR amplification, was induced to the target gene.

为构建融合蛋白表达载体和获得与天然蛋白质序列完全一致的目的蛋白，以含有目的基因的重组质粒DNA为模板，设计引物时加入两端酶切位点及肠激酶裂解位点，通过PCR扩增引入目的基因中，测序结果表明接头和读框正确。

The objective of the study was to obtain the gene of bovine enterokinase light chain,which would be used in the cleavage and purification of fusion proteins.The fragment of bovine enterokinase light chain cDNA was obtained by RT-PCR from a sold bovine′s duodenal mucosa, then cloned into the pUCmT cloning vector and sequenced.Compared with the sequence deposited in GenBank,the cloned gene sequence is correct.

为克隆表达牛肠激酶轻链编码基因，以期应用于融合蛋白的切割与纯化，从市售北方黄牛十二指肠组织中提取总RNA，以RT-PCR方法扩增其cDNA片段，将此片段克隆于pUCmT载体中，经过特异性限制性内切酶酶切分析片段正确后，进行全序列分析。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。