查询词典 Gene

In this research we obtained the banana ACO gene with about 1.2kb length by cuffing from the cloning vector pUCm-ACO with restriction endonucleases Xba Ⅰ and Sac Ⅰ, and then directionally linked the gene to the plasmid vector pBⅠ-121 in the same endonucleases digestion sites, finally we got the construct of plant expression vector pBI-aACO.

用限制性内切酶Xba Ⅰ和sac Ⅰ从克隆载体pUCm-ACO上切下约1.2kb的香蕉ACO基因，将其定向连接在经相同酶切的质粒载体pBⅠ-121上，构建成植物表达载体pBⅠ-aACO。

RNA interference represents a common biological phenomenon of double-stranded RNA mediated post-transcriptional gene silencing, which lies in altitude plants and animals. The mechanism of this process is described as that: the dsRNA in cells are cut into double-stranded small interfering RNA. By joining with an effector complex termed RISC (RNA-induced silencing complex), siRNA binds to the cellular RNA with homologous sequences, and contributes to the degradation of the corresponding mRNA, the inhibition of specific gene expression.

RNAi技术是双链RNA(double strand RNA，dsRNA)介导的序列特异性的转录后基因沉默，广泛存在于高等植物和动物中，其机制是细胞内的dsRNA被Dicer剪切成双链的小分子干涉RNA (small interfering RNA，siRNA)与RNA诱导的基因沉默复合物结合，降解与之互补的靶mRNA，特异性抑制靶基因表达。

Signal Transduction , Gene Isolation and Gene Expression Involved in Cytogenesis

中科院微生物研究所；系统生物工程研究所

By sequencing randomly-selected clones from the library and usingbioinformatics, 73 novel expressed sequence tags were obtained, in which therewere 24 full-length cDNAs, including cytolysin gene, neurotoxin genes, and greenfluorescence gene. Functional study on these novel genes is an ongoing task in ourlaboratory.

对文库克隆进行序列测定和生物信息学分析，已经获得了73个海葵新表达序列标签，有24个全长cDNA，其中包括海葵细胞毒素、海葵神经毒素、绿色荧光蛋白等具有开发应用价值的新基因。

OBJECTIVE: To explore the gene augmentation of cytosine deaminaseto NSCs as well as its gene expression.

目的：探讨胞嘧啶脱氨酶基因修饰神经干细胞及其基因表达。

OBJECTIVE: To investigate liposome-mediated cytosine deaminase gene transfecting rabbit BMSCs and its gene expression.

目的：探讨脂质体介导胞嘧啶脱氨酶基因转染兔骨髓间充质干细胞及其基因的表达。

For cloning antifreeze protein gene from a desert darkling beetle Microdera punctipenis dzunarica in Xinjiang, the primers were designed according to the core sequence of AFP gene deposited in GenBank, and the cDNA fragment about 294 bp named as MpAFP5 was amplified with the RT-PCR and 3'-RACE technique. Sequence analysis revealed that the cloned cDNA fragment named as MpAFP5 coded the mature peptide of AFP.

根据GenBank中已发表的昆虫抗冻蛋白基因的保守序列设计引物，利用RT-PCR技术结合3'-RACE扩增的方法，从新疆荒漠昆虫准噶尔小胸鳖甲Microdera punctipenis dzunarica中获得了长约294 bp不含信号肽的抗冻蛋白cDNA片段，命名为MpAFP5，其全长序列为363 bp(GenBank注册号为：AY821792)。

A lot of people do not know , the aspirant that drives for sudden huge profits people already darkling built empire of a gene , covet the gene glom on to complete mankind .

许多人不知道，为暴利驱使的野心家们已在暗中建立起了一个基因帝国，妄想把全人类的基因据为己有。

RESULTS: We cloned cytosine deaminase gene and the results from gene sequencing were the same as Genbank.

将胞嘧啶脱氨酶基因亚克隆到pIRES2-AcGFP1质粒上，构建了pIRES2-AcGFP1-CD真核表达载体。

Comparisons of mitochondrial gene arrangements of decapod suggested that rearrangement of the four gene-block found in Eriocheir is informative for high level phylogenetic study of decapod.

十足目动物线粒体基因排列顺序的比较研究显示，在绒螯蟹中发现的4基因块的重排，对十足目高级阶元的系统发生研究是有用的信息。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。