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To better understand the roles of LsrA protein in nodulation, a series of analyses of nodules using scanning electron microscopy, genetic and biochemical approaches have been carried out. Our analyses suggest that the lsrA1 nodules contain bacteroids in the invasion and establishing zone only. The lsrA gene expression is active early in the invasion zone and activated later than bacA genes. The lost of LsrA protein dramatically reduced the expression of nifA and fixK, and completely blocked the expression of the nifH gene for nitrogenase. LsrA protein functions early in the bacteroid development and it is essential for the development nitrogen fixing bacteroids.

前期的工作中，苜蓿中华根瘤菌Rm1021中90个候选LysR基因已经被定向插入突变，并筛选在自生生长时期、共生生长时期的表型，以期寻找更多在自生状态或共生固氮中有功能的LysR转录因子。1 针对前期鉴定出的共生固氮必需的lsrA基因，我们应用了一系列扫描电子显微镜技术、生物化学、分子遗传学等方法，发现lsrA基因主要在根瘤侵染区开始表达，表达时序也在侵染阶段左右，但晚于bacA基因表达；LsrA蛋白缺失后根瘤固氮区中缺乏具有固氮能力的类菌体，nifA和fixK基因的转录水平降低，nifH基因的转录被完全阻断，因此LsrA蛋白为根瘤发育所必需，是新的根瘤发育信号传导途径成员。2 通过表型筛选我们鉴定了苜蓿中华根瘤菌的oxyR基因，并研究了它的调节特性。oxyR突变后，苜蓿中华根瘤菌对过氧化氢敏感性提高，适应性降低。

Early spring uses "to protect sets up general" 100 time of fluids + Baume 3 lime sulphurs clear gardens disinfection, not only the sterilization extinguishes the insect, but also can suppress the extraneous source gene stable heredity, thus stimulates a series of defense response, causes the fruit tree to have the intense resistance, at the same time may hit stirs up the fruit tree "the gene alarm clock", when does the reminder fruit tree regain consciousness from the hibernation, when germinates, blossoms, the sensitive plant nerve, causes the fruit tree vegetal period to be full of vigor.

早春用"护树将军"100倍液+波美3度石硫合剂清园消毒，不但杀菌灭虫，还能抑制外源基因稳定性的遗传，从而激发一系列防卫反应，使果树产生强烈抗性，同时可撞激果树的"基因闹钟"，提醒果树何时从冬眠中苏醒，何时发芽，开花，敏感植物神经，使果树生长期精神饱满。

The in vivo growth of melanoma could be controlled by OSM secreted from OSM gene-modified cells. The activated Egr-1R by radiation could enhance the expression of OSM and thereby impose an aggravated damage on tumors in vivo, namely brining a dual inhibitory effect of gene and radiation on tumors.

转基因分泌的OSM可对瘤细胞的体内生长产生抑制；射线可通过Egr-1R增强OSM表达的途径加剧肿瘤抑制，即基因与射线的双重抑瘤效果。

We used NCAR/NCEPT anew analyze data and the method of bilinearity insert to fix on profile distribution of several physical field in the air on observation point and the stream field of 700hPa. We primary discussed the possible mechanism to influence aerosol size distribution.The atmospheric condition of 1000hPa~700hPa level had great influence on aerosol size distribution, and influence on coarse particle mainly below 850hPa level. The horizontal wind velocity of 925hPa level is a primary gene which contributed to coarse particles in the air. Vorticity profile especially below 700hPa level is a key gene to influence diffuse and convergence of local aerosol. The air vertical motion velocity greatly influenced aggregation and remove of aerosol particles. The vertical distribution of air temperature mainly influenced vertical transportation of aerosol.

利用NCAR／NCEP逐日再分析资料，通过双线性插值方法，确定观测点上大气中几个物理量的廓线分布；及700hPa的流场，初步探讨了影响大气中气溶胶粒子谱分布的可能机制： 1000hPa～700hPa的大气状况对气溶胶粒子谱分布影响显著，对粗粒子气溶胶的影响显著的主要集中在850hPa以下；925hPa的水平风速是影响大气中粗粒子气溶胶的一个主要因子；涡度廓线(尤其是700hPa左右及以下的涡度廓线)是影响局地气溶胶的扩散的一个关键因子；大气的垂直运动速度对气溶胶的聚合及移出影响较大；大气温度的垂直分布主要影响气溶胶垂直输送。

In the argument between invention and discovery on patent of biotechnology .I ,firstly, take back look at the definition of invention and discovery in practice of patent of biotechnology in western countries . then ,analyse the possibility of putting the result of basic bioresearch into patent .Finally ,draw a conclusion :we should focus on the achievement of research on natural material and gene invention should be patented as long as the material and gene which have been separated and purified have real value .

关于生物技术专利中的发明与发现之争，本文首先回顾了西方发达国家在生物技术专利实践中对发明与发现的界定，接着分析了生物技术基础研究成果的可专利性，最后得出结论，我们应淡化发明与发现之分，着重于"三性"审查，即只要天然物质和基因被分离和纯化以后具有工业实用性，就应该给天然物质和基因发明等基础研究成果授予专利。

The colony forming and the cell cycle were analyzed with flow cytometry, and the mRNA expression of bcl-2, c-myc gene were determined with RT-PCR method. Results Resveratrol could markedly inhibit proliferation of human gastric cancer cells compared with that of the blank control group. The cell cycle was blocked to G0/G1. The mRNA level of bcl-2 and c-myc gene were decreased compared with that of the blank control group.

结果 白藜芦醇可抑制胃癌细胞增殖和集落形成能力，低、高剂量组的抑制率分别为6.9%，17.6%和27.98%，63.39%，差异均有统计学意义(P.05)；白藜芦醇能使胃癌细胞周期阻滞于G0/G1期，明显下调胃癌细胞bcl-2、c-myc基因mRNA表达水平(P.05)；用药24 h后，白藜芦醇高剂量组bcl-2和c-myc基因相对表达量分别为(0.54±0.04)，(0.58±0.05)，与空白对照组(0.91±0.05)，(0.85±0.03)比较，差异均有统计学意义(P.05)。

We studied the optimizaiton, accuracy and reliability of this technique by definition the genotype of five common mutation loci of β-globin gene in the carrier with CD41-42 mutation and that of β-globin gene of normal people's single lymphocyte and single blastomere.

我们采用了一种利用单细胞标本通过二重巢式PCR并结合ARMS技术确定中国人群中广泛的β-地中海贫血突变谱的基因型的策略，通过对含有β-珠蛋白基因CD41-42突变的携带者5个该基因常见突变位点基因型的确定，以及正常人的单个淋巴细胞、单卵裂球的β-珠蛋白基因该5个位点基因型的确定，对这种技术条件的优化、准确性和可靠性进行了研究。

The coding region of Bar gene, the left border of Ds element, the coding region of GUS gene

粒p13B，用于分离水稻基因启动子。以此质粒用农杆菌介导的方法转化粳稻品种中花11 的

American molecular biologist Cohen, the United States biochemists Boyer will be completed with the cooperation of the two different gene splicing genes into bacteria composite Experimental and declared the first gene recombination technology patents.

美国分子生物学家科恩、美国生物化学家博耶合作完成了将两种不同基因拼接的复合基因引入细菌的实验，并申报了第一个基因重组技术专利。

According to either sequence data sets or gene arrangement features at both gene junctions, Brachyuran crabs were monophyletic and divergent between Heterotremata and Thoracotremata. Potamoidea represented by S.

本文基于基因序列和排列数据的系统发生分析都支持短尾类是一单系群；异孔类和胸孔类互为姐妹群；以长江华溪蟹为代表的溪蟹总科与扇蟹总科、梭子蟹总科有较近的亲缘关系。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。