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We submitted the gene fragment to GenBank and its accession number was DQ346681. The results of BLAST showed that the gene fragment has 100% identification with turkey and chicken and 96% with human, monkey, swine, horse and dog.

本研究中的鹅OBR基因片段序列与火鸡和部分鸡的OBR基因序列同源性达100%，与人、猿、猪、马、犬等哺乳类单胃动物的同源性都达到96%。

The SPAG8 gene was a new gene and assigned the accession number U12978 by GeneBank.

原位杂交实验显示SPAG8基因位于人类第9号染色体的短臂上。

The accession number of this gene is EU590683. The sequence of this gene was arranged and analyzed with Mega software package having a phylogenetic tree built up in DNADist and Neighbor.

此外，采用Bioedit和Mega2软件构建了Fe-氢酶的NJ系统进化树，结果表明该Fe-氢酶基因与产气荚膜梭菌聚为一类。

PPT acetyl transferase gene was the report gene in the experiment.

PPT乙酰转移酶基因在实验中作为报告基因。

Active MAPKs translocate into nucleus and thus regulate gene expression. Now, three main parallel pathways of MAPK family have been discovered : ERK1/2, JNK and p38. They can induce different gene expression and thus cause different cell reaction.

现已发现MAPK家族主要有三条平行的信号转导通路：ERK1／2、JNK和p38，三条通路可以活化不同的转录因子，启动不同的基因表达，从而引发不同的细胞反应。

The level of ADAR gene expression was expressed as ratio expression rate of ADAR gene /β-actin acc...

ADAR3在正常人脑胶质细胞及苯乙酸处理前后的胶质瘤细胞中，均未见表达。结论ADAR2 mRNA水平高表达，可能与高恶性度胶质瘤的发生有关。

To obtain the VPg gene of PRSV-W, the VPg gene of PRSV-P was modified into a VPg gene of PRSV-W by long primer extension and overlap extension PCR.

以此为基础，利用长引物延伸和套叠PCR的方法，定点突变剩余5个位点，最后拼接获得W型PRSV-VPg基因。

Simply assuming that under different conditions, gene expression levels have three regulation states, i.e., up-regulation, down-regulation and non-regulation states, we can probabilistically establish a model of gene regulation states to compute gene regulation probabilities under different conditions.

简单假设在两种不同的实验条件下被调控的基因表达水平表现为三种调控状态，即上调控状态、下调控状态和非显著调控状态，我们使用概率统计方法对基因调控状态进行建模。在该模型的基础上，我们应用三种参数概率估计方法对基因调控概率进行估计。

The further analysis indicated that the double gene transgenic petunia possessed stronger capacity to take up liquid formaldehyde than the control and the single gene, DAS or DAK, transgenic plants. This resuLt suggests that the expressed DAS and DAK might establish aHCHO-fixation pathway which functions in the double gene transgenic plants.

对液体甲醛吸收速率测定结果表明转DAS、DAK双基因的矮牵牛对甲醛的吸收速率明显快于对照植物和转单个基因DAS或DAK的植物，说明转基因表达产生的DAS、DAK可能构成一条甲醛固定途径，在转双基因植物中发挥作用。

The apxⅢA gene of Actinobacillus pleuropneumonie was amplified by PCR. The amplified DNA fragment 3 466bp was cloned into pMD18-T. After R.E. analysis and sequencing, the apxⅢA gene in pMD18-T was ligated into pBluescripⅡSK, the recombinant expression plasmid pET-28b/apxⅢA was constructed and analysed with R.E., the protein of apxⅢA gene expressed in E.coli BL21 was detected by Western blotting.

因胸膜肺炎放线杆菌的致病性主要是由毒素决定的，故参照猪胸膜肺炎放线杆血清2型菌株的序列(GenBankL1 2 1 4 5)设计了一对特异性引物，用PCR的方法扩增apxⅢA基因并得到了长 3 466bp的片段，然后将其克隆到pMD 1 8T中，经酶切鉴定和序列分析表明克隆是成功的；再将apxⅢA插入到原核表达载体pET 2 8b后，转化BL2 1 (DE3)，在IPTG诱导下获得高效表达，经Westernblotting检测证实表达产物有活性。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。