查询词典 Gene

But there are no correlations between gene expression and gene expression specificity or gene expression level.

有趣的是，基因的组织特异性和基因的表达量与基因表达的进化则可能没有相关性。

Methods PCR amplification of drug resistance gene abeM was carried out using chromosomal DNA of Ab 35 and Ab 36 clinical isolates as template. PCR product of abeM gene was sequenced. The nucleotide sequences and deduced amino acid sequences were analyzed by BLAST of NCBI. After cells of Escherichia coli KAM32 transformed with a plasmid carrying the abeM gene PCR product, MICs of various drugs were determined by serial twofold dilution method.

以临床分离的Ab 35、Ab 36染色体DNA为模板扩增abeM基因，对abeM基因扩增产物进行DNA测序及同源性分析；将abeM基因扩增产物与载体连接后转化入大肠埃希菌KAM32感受态细胞中，对转化子进行药敏分析。

To protect the identity of orchid cultivar and the right of the breeders, we successfully develop a novel method by the introduction of foreign DNA sequence (27bp) as molecular marker via BioWare low pressure gene gun into Mormodes lawrence and Zygopetalum mackayi. Histochemical GUS transient expression was obtained from aleurone layer and embryo of Zea mays using BioWare low pressure gene gun with acceleration pressure of 50psi. This is the first report on successful transfer into plant tissue using BioWare low pressure gene gun.

本研究的目的为利用核苷酸排列组合之多样性，设计一段27个碱基对序列来代表品种所有者注册资料，经由国人自行研发之生物镓低压基因枪，利用50psi的氦气压力，将设计好DNA序列标志转殖至妖精兰与轭瓣兰品种，使其实生或分生之子代，皆会具有此注册片段，品种权所有者可以具体以PCR或定序分析确认DNA序列标志，作为保障种源权之认证系统。

Main Ingredient: various plant elite, for instance, nucleic acid gene renewal gene, natural moisture gene, wheat embryo oil, aloe extract, witch hazel extract, almond oil ,etc

主要成份：核酸基因修复因子、天然保湿因子、小麦胎芽油、芦荟提取液、金缕梅提取液、杏仁油等多种植物精华。

By Nothern blot, this gene band located between 1383-1908bp maker, length of which was same with different gene obtained by subtractive hybridization. The results showed that the obtained wnt6 gene was a cDNA fragment with full open reading frame, which could be useful for further studies.

提取样本总RNA，与地高辛标记的Wnt6探针进行杂Northernblot杂交，结果阳性杂交带位于1383-1908bp处，与差异片段大小一致，验证了该基因确为全长cDNA片段，具有完整的开放读框，可以用于进一步研究。

Selecting the better amplified from Diacylglycerol acyltransferase, gene, Acyl- desaturase gene ,then cloning sequencing, afer that, through the NCBI and nucleotide sequence to match homology , the results show that: cloning by the cassava, castor-oil plant Jatropha curcas DGAT and SAD genes and gene fragments have the high homology with other known plant DGAT genes and SAD genes .

从中筛选出扩增效果较好的二酰基甘油酰基转移酶(Diacylglycerol acyltransferase， DGAT)基因，硬脂酰-酰基载体蛋白脱饱和酶(Acyl- desaturase， SAD)基因克隆测序，经测序，通过NCBI与已知的核苷酸序列进行同源性比对，结果表明：克隆所获得的木薯、蓖麻和麻疯树 DGAT基因和SAD基因的片段与其它已知植物的DGAT基因和SAD基因具有很高的同源性。

Selecting the better amplified from Diacylglycerol acyltransferase, gene, Acyl- desaturase gene ,then cloning sequencing, afer that, through the NCBI and nucleotide sequence to match homology , the results show that: cloning by the cassava, castor-oil plant Jatropha curcas DGAT and SAD genes and gene fragments have the high homology with other known plant DGAT genes and SAD genes .

从中筛选出扩增效果较好的二基甘油基转移(Diacylglycerol acyltransferase， DGAT)基因，硬脂-基载体蛋白脱饱和(Acyl- desaturase， SAD)基因克隆测序，经测序，通过NCBI与已知的核酸序列进行同源性比对，结果表明：克隆所获得的木薯、蓖麻和麻疯树 DGAT基因和SAD基因的片段与其它已知植物的DGAT基因和SAD基因具有很高的同源性。

Label with LC-Red640 and LC-Red750 two are aimed at above all 112 code with 158 child detect 5 ′ carry bougie, corresponding anchor decides bougie to be labelled by fluorescence in 3 ′ end, circulate quickly from inside DNA of person gene group again PCR is distinctive the enlarge that make thing adds part of ApoE gene 265bP and with distinctive bougie cross hind, cause fluorescent resonance energy to transfer, the occurrence that passes different source cross and fault match alkaline base the fusible parameter of the type will finish feral model divide with choppy gene model.

首先以LC-Red640和LC-Red750标记两条针对112和158位编码子的检测探针5′端，相应的锚定探针在3′端被荧光标记，再从人基因组DNA中快速循环PCR特异引物扩增apoE基因265bP片段并和特异探针杂交后，引发荧光共振能量转移，通过异源杂交的出现和错配碱基的类型的熔解参数来完成野生型和突变基因分型。

In the functional mechanism, we should use the technique "use the DNA Microarray to build the gene expression difference chart" to lead to the combination of Traditional Chinese Pharmacy and gene expression difference chart to study. Meanwhile,we can establish a totally new method of screening modern Traditional Chinese Pharmacy based on the gene expression difference chart, it can really make the modernization and internationalization of Traditional Chinese Pharmacy.

在中药作用机制方面，应采用&利用基因芯片建立基因表达差异谱&技术，将中药与基因表达差异谱有机地结合起来进行研究，同时以基因表达差异谱为基础建立一种全新的现代中药筛选方法，使中药真正实现现代化、国际化。

An expression construct specified in mammary gland with double cistrons of human G-CSF gene and IRES-EGFP gene under control of ovine beta-lactoglobulin gene flanking sequence,has been constructed in two parts (named fragment I and Fragemnt II)that share an overlapping region of 2.2 kb sequence.Two sites of loxp and lox2272 for homologous recombination were inserted into both flanking regions of G-CSF.The lengths of fragment I and Fragment II are 5.9kb and 5.6 kb,respectively.The whole length of the expression vector (β-LG-hG-CSF-IRES-EGFP) is 9.3 kb.

以绵羊β乳球蛋白基因（β-Lactoglobulin，β-LG）为转基因表达框架，将人G-CSF(Human Granulocyte Colony Stimulating Factor， hG-CSF)基因与报告基因-增强型绿色荧光蛋白(Enhanced Green Fluorescenct Protein， EGFP)基因作为双表达单元拼接到β-LG基因的第一外显子处，并在G-CSF基因两侧引入同源重组位点loxP、lox2272，将打靶基因表达构件β-LG-hG-CSF-IRES-EGFP(总长9.3 kb)分为两段进行构建，片段Ⅰ（长：5.9kb）与片段Ⅱ（长5.6 kb）两段重叠部分为2.2 kb。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。