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Methods After the wide type NCI-H446 cells and the three kinds of NCI-H446 cells transfected by three kinds of IL-15 genes respectively （Cmp transfected by the IL-15 maturation peptide gene, Cp transfected by prototypic IL-15 gene, and Csp transfected by modified IL-15 gene whose signal peptide was replaced with the IL-2 signal peptide） were treated by mitomycin, the cells （named MCw, MCmp, MCp, and MCsp, respectively） were used as stimulator cells to stimulate PBMC from healthy volunteers.

野生型NCI-H446细胞和被3种IL-15基因分别转染的3种NCI-H446细胞（Cmp：被IL-15成熟肽基因转染；Cp：被原型IL-15基因转染；Csp：被信号肽换为IL-2信号肽的改型IL-15基因转染），用丝裂霉素处理后（分别名为MCw、MCmp、MCp和MCsp）作为刺激细胞刺激健康志愿者的PBMC。

PartⅠ The Expression and Location of HCK Gene During the Development of Rattus Heart Objective To investigate the mRNA expression of HCK gene in the developing rattus heart and to analyze the relationship between HCK gene expression and the heart development.

目的检测HCK基因在大鼠胚胎心脏发育过程中mRNA表达的变化，分析其与心脏发育的关系。

The expression of AT2R gene can be regulated efficiently in vivo by local delivery of MSC with dual-stable expression of AT2R gene, thus reduces the formation of intimal hyperplasia.④AT2R gene can influence the expression of ECM and significantly promote the cellular apoptosis in the injury rat carotid artery.⑤These data demonstrate the clinical potential of AT2R regulatable expression to prevent restenosis after PCI.

研究结论：(1)与VSMC直接接触可以诱导MSC向血管成分细胞分化；(2)构建的Dox-on可调控的MSC细胞株诱导活性可靠，使外源AT2R基因的表达处于有效的主动控制下；(3)局部导入MSC不影响球囊损伤后大鼠颈动脉新生内膜增生；(4)由MSC介导的AT2R可调控表达受到Dox的良好调控，可有效抑制球囊损伤后大鼠颈动脉新生内膜增生；(5)AT2R影响血管损伤后修复时ECM成分的表达，并促进细胞凋亡的发生，可能是AT2R介导抑制新生内膜形成的机制。

To investigate the characteristics of the S gene in almond,we degenerative primers according to conservative sequence of S gene in rosaceous plant and got 10 S gene fragments by PCR application from 8 almond cultivars in China.

根据蔷薇科树种S基因的保守序列设计引物，利用PCR技术对8个新疆扁桃品种S基因进行扩增，获得了10个大小不同的S基因片段。

rutaecarpine pharmacological effects and its related mechanism: rutaecarpine the heart positive inotropic, positive changes in the role and the heart protective effect, which with its promotion of calcitonin gene-related peptide release; rutaecarpine alkali have vasodilative and hypotensive effect, its mechanism mainly related to endothelial cells Ca2 +-NO-cGMP pathways and calcitonin gene-related peptide release; rutaecarpine to relax the anal sphincter, gastrointestinal motility inhibition of stress ulcer and ulcerative colitis have therapeutic effect; rutaecarpine on cerebral ischemia-reperfusion injury has a protective effect, which instead of increasing the concentration of calcitonin gene-related peptide-related; rutaecarpine inhibits platelet aggregation and arterial thrombosis; In addition, rutaecarpine Alkali also anti-inflammatory and analgesic, anti-tumor and other pharmacological effects.

综述了吴茱萸次碱的药理作用及其相关机制：吴茱萸次碱对心脏有正性肌力、正性变时作用和心脏保护作用，这与其促进降钙素基因相关肽释放有关；吴茱萸次碱有扩血管和降压作用，其机制主要涉及内皮细胞Ca2+-NO-cGMP途径和降钙素基因相关肽的释放；吴茱萸次碱能松弛肛门内括约肌，抑制胃肠运动，对应激性溃疡和溃疡性结肠炎也有治疗作用；吴茱萸次碱对脑缺血再灌注损伤有保护作用，这与其增加降钙素基因相关肽浓度有关；吴茱萸次碱能抑制血小板聚集和动脉血栓形成；此外，吴茱萸次碱还有抗炎镇痛、抗肿瘤等药理作用。

Except on the testis tissues,the expression of ZFY gene were found on the kidney tissues of human embryo or rat, brain vesicles〔rat embryo 13 days〕,and sclerotome cells〔rat embryo 13 days〕 It is possible that because kidney and genital system come from same origin,ZFY gene expressed on the kidney tissues too, the expressive function of ZFY gene on the embryonic brain vesicles and sclerotome cells is not yet clear.

除了在睾丸组织上表达外，ZFY基因还在肾脏组织及鼠胚脑泡(胚胎13天)，生骨节细胞(胚胎13天)上表达。在肾组织上表达可能是肾脏与生殖系统起源相同有关。在胚脑泡及生骨节细胞上表达其作用不清楚。

The results showed that there were differences among treatments in transformation of high quality gene(Glu\|D 1 5+10) in F 1 grains, and heterozygous type was more frequently appeared in the treatment of irradiation. In the F 2 segregative population, heading date deviated to early\|mature parent in the treatment of reciperocal cross and its combination with irradiation, indicating that cytoplasm and irradiation could promote the heading. In F 3 the expressive frequency of (5+10) gene in the cross was lower than that in reciperocal cross, and irradiation treatment could raise the expressive frequency of(5+10) gene. These results showed varience in different crosses.

结果表明，不同组合处理间F1籽粒优质亚基(Glu D15+1 0 )的转化存在差异，辐射处理的多以杂合型出现。F2 分离群体中，反交及反交结合辐射处理的抽穗期明显倾向于早熟的亲本，细胞质与辐射具有促进抽穗期的作用。F3 植株Glu D15+1 0亚基的表达频率，不论是哪种组合，反交时 5+1 0亚基的表达频率高于正交；无论正交还是反交，辐射处理提高了 5+1 0亚基的表达频率，而且在不同组合间存在差异。

The N gene protein was expressed in the CHO cells proved by IFA. The N gene was transcripted as proved with RT-PCR. CHO/CDV-N cell strain was constructed, which provided evidence for research of CDV serumal detection and gene vaccine.

然后通过磷酸钙共沉淀法转染CHO-K1细胞，通过潮霉素筛选得到阳性克隆，间接免疫荧光实验鉴定N基因在CHO细胞中的表达，并用RT-PCR方法从转录水平证实N基因在CHO-K1细胞中的表达，最终建立了CHO/ CDV-N细胞株，为犬瘟热病毒的血清学检测和基因疫苗的研制奠定了基础。

Sequence analysis and homology alignment showed that MD1 had 93% similarity with matK in maize chloroplast genome, a gene encoding maturase included in type Ⅱ intron splicing of RNA transcript; MD2 had 99% similarity with gene PP2C, encoding serine / threonine protein phosphatase type 2C in extremely drought tolerant Sporobolus stapfianus; and MD3 had 99% similarity with rice gene encoding metacaspase, belonging to aspartic acid specific cysteine caspases.

序列分析和同源性比对表明，MD1与玉米叶绿体基因组中编码参与RNA转录本Ⅱ型内含子剪切的成熟酶的matK基因有93%的相似性，MD2与极端耐旱的Sporobolus stapfianus的丝氨酸／苏氨酸2C型蛋白磷酸酶基因PP2C的相似性达99%，MD3与属天冬氨酸特异性半胱氨酸蛋白酶类的水稻metacaspase基因有99%的相似性。

Results of sequence and homology analysis show that MD 1 has 97% similarity with mark in maize chloroplast genome,a gene encoding RNA maturase involved in group Ⅱ intron splicing of RNA transcript;MD2 has 99% similarity with the gene serine/threonine phosphorylase 2C in Sporobolus stapfianus;and MD3 has 99% similarity with rice the gene encoding metacaspase,an arginine/lysine-specific cysteine protease.

序列分析和同源性比对表明，MD1与编码成熟酶的玉米叶绿体基因matK有97%的相似性，MD2与极端耐旱植物Sporobolus stapfianus编码丝氨酸/苏氨酸蛋白磷酸酶的PP2C基因有99%的相似性，MD3与属精氨酸/赖氨酸特异性半胱氨酸蛋白酶类的水稻metacaspase酶基因有99%的相似性。根据MD2片段序列，结合电子克隆和RT-PCR方法，克隆出一条1731 bp的全长cDNA序列，它编码388个氨基酸。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。