查询词典 Gene

The further study will identify the defected gene of the lamellar cataract, and make it possible for gene diagnosis and gene therapy.

进一步的研究工作将明确其致病基因，使该病的基因诊断和基因治疗成为可能。

Signal peptide sequence was removed lest it couldnt be recognized by Bacillus subtilis, gene coding for mature peptide was ligated to downstream of sacB signal peptide sequence of pWB980 to form a new ORF and generate pWB980-LipA, gene coding for its chaperone was also amplified and ligated to the downstream of LipA to generate a secretion/expression vector pWB980-LipAB.thechaperone gene was sequenced and analyzed by multiple alignments, resulted showed that there were mutations of nucleotides(1～7) and amino acids (1～2) with the other reported genes.

为了防止枯草芽孢杆菌信号肽酶不能识别脂肪酶的信号肽，切除掉脂肪酶的信号肽编码序列，与枯草芽孢杆菌分泌表达质粒pWB980连接并与质粒上的SacB信号肽构成一个完整的开放式阅读框，获得重组质粒pWB980-LipA，因为该脂肪酶的活性表达需要一个特异性分子伴侣帮助折叠成有活性的构象，将脂肪酶分子伴侣基因串连到脂肪酶基因的下游，获得重组分泌表达载体pWB980-LipAB并且测序分析分子伴侣的基因序列和氨基酸序列并与其它报导的序列进行了比对，发现有1到7个碱基的差异和1到2个氨基酸残基的突变。

The expression of cloned cold-adapted lipase gene lipA in Pichia pastoris GS115 with the pPIC9k as the expressive vector was performed according to the methods described in the manual, version 3.0, of the pPIC9K identified the lip gene cloned here was a functional lipase gene.

以pPIC9K为表达载体，以GS115为表达宿主菌，成功构建重组表达质粒pPIC9K-lipA，经甲醇诱导表达，能够分泌有脂肪酶活性的表达产物，因此证明本研究所克隆的脂肪酶基因lipA确实为脂肪酶开放阅读框。

It was then introduced by gene pulser into acrystalliferous strain CryB and a recombinant strain CryB (pBMBZGC10) was obtained. Different fermentative solutions of recombinant strain were used for multi-spray on leaves of Brassica pekinesis, Ipomoea aquatica and Lycopersicon esculentum. Results by using fluorescent detection and PCR amplification revealed that cry1Ac10 gene could not been detected in roots, stems and leaves of plants tested. The cry1Ac10 gene was also not transferred into indigenous bacteria, actinomyces and fungi in soil.

苏云金芽胞杆菌标记重组菌株与杀虫基因水平转移的研究将重组菌株CryB（pBMBZGC10）的发酵液按浓度梯度分次进行喷洒小白菜、蕹菜和番茄作为供试植株，利用标记基因研究的结果表明：cry1Ac10基因没有向供试土壤细菌、真菌和放线菌转移，也未在植物的供试植物根、茎和叶中检测到该基因。

The DNA sequences around the transposon inserted site were also amplified by inverse PCR and sequenced. Multisequence alignments showed that the mutated genes were highly similar to the malonyl coenzyme A transacylaseg gene, lipopeptide synthetase B gene and sensor histidine kinase gene, respectively.

反向PCR克隆转座插入位点周边序列及测序结果表明，转座突变的基因与编码脂肽类化合物合成和调控相关的malonyl coenzyme A transacylase、lipopeptide synthetase B和sensor histidine kinase蛋白基因具有高度同源性。

Objective: The genes were connected basing on obtain gene of MCP and DAF of liver and fetal marrow of Chinese adult and then design mammiferous vector of the hMCP-DAF fusion gene. Finally transfer hMCP-DAF fusion gene to the early embryo of jinhua''s pig.

目的 在获得中国成人肝脏、胎儿骨髓MCP、DAF基因克隆的基础上，进一步将两个基因串接，并构建hMCP-DAF融合基因的哺乳动物表达载体，筛选出具表达活性的hMCP-DAF并导入金华猪早期胚胎。

Among all, deleted in liver cell (DLC-1) gene was found to play an important role in hepatoma in 1998, and after that hypermethylation on the promoter of the DLC-1 gene was found in many other cancers, suggesting that epigenetic modification is another way to control the carcinogenesis. Another gene, Endothelin type B receptor, was found to cause megacolon syndrome by accident while searching the cause of white spot in mouse, and was also found hypermethylation on its promoter in some cancer cells.

在此中，DLC-1基因自从1998年后被发现在肝癌的演化上扮演重要的角色后，在许多其他的癌症上也发现此基因上位於promoter的CpG islands有过度甲基化的现象，推论DLC-1基因的过度甲基化是一种经由epigenetic modification调节细胞癌化的方式。

The result indicates that there are one dominant and one recessive complementary gene that are effective against the Indian race 2E16. However, the monosomic analysis results indicated there was one resistant gene to the Indian race 2E16 and the gene was located on chromosome 3B. The current study shows that it is different from the known genes.

采用常规杂交分析方法，对铭贤169与阿夫杂交的F_1、F_2、BC_1代及其亲本进行抗性鉴定和统计分析，结果表明，阿夫对2E16菌系的抗性至少由1对显性抗条锈基因控制；通过单体分析将阿夫抗2E16菌系的1对显性基因定位在3B染色体上，等位性分析表明该基因不同于目前的已知基因。

The Sry gene sequence of Muntiacus crinifrons was acquired and the conclution was that there are 83% homology between the human and Muntiacus crinifrons. It was testified that in all mammal Sry gene is consertive. On the other side the Sry gene was located to the Y2 chromosome of the Muntiacus crinifrons.

最后提取雄性黑麂的基因组DNA，并用同一对引物对其进行扩增，亦得到Sry基因的片段，对此扩增片段进行克隆，测序，结果表明其与Y2染色体得到的Sry基因片段完全一样，与人SRY基因的同源性均为83%。

They all contain 13 protein coding gene,2 rRNA gene,22 tRNA gene and 1 D-Loop.The base composition for the four nucleotides is A-32.0%,C-27.6%,G-14.7%,T-25.8%,And it is A-32.5%,C-26.9%,G-14.1%,T-26.5%for Yellow-throated Marten.But there are some definite differences in base composition,the using of Initiation codon and Stop codon,and the mode of repeat sequences in control region.The codon usages of Manes have bias,and the ttiird locations of codon of protein-coding genes have the higher frequency in using A and C.There may be some relativity with the content of A and C in D-loop,namely,it has relation with the mode of repeat sequences.The complete mitochondrial genome of the Sable and Yellow-throated Marten were submitted to GenBank,and the accession number are FJ429093 and FJ719367 respectively.3、The complete mitochondrial genome of 6 other species of Mustelidae from GenBank and some sequences of D-loop from the 6 species were aligned.

分析紫貂大兴安岭亚种、长白山亚种、阿尔泰亚种和北欧亚种间的基因流及进化历史得知：大兴安岭种群与新疆种群和长白山种群间的基因流水平最高(Nm=0.1260和0.1427)，新疆与长白山种群间最低Nm=0.0053紫貂种群在进化过程中可能发生过种群膨胀，经历过种群增长过程。2、对紫貂和黄喉貂的线粒体全基因组结构进行分析发现：全长分别为16 523bp和16549bp，均包含13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和1个非编码序列区(D-Loop区，紫貂全序列中碱基组成为A-32.0%，C-27.6%，G-14.7%，T-25.8%，黄喉貂为A-32.5%，C-26.9%，G-14.1%，T-26.5%；基因排列顺序与日本貂和貂熊的一致，但碱基组成、起始密码子和终止密码子的使用及控制区中串联重复序列模式等均存在一定差异。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。