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The predicted amino acid sequence of the gene show a rather high homology(84.47% amino acid identities)with Haemaphysalis longicornis(H.longicornis) TnI (GenBank,GI:14041807).Furthermore, the actin-binding domains of troponin I in predicted amino acid sequence, just the same as H. longicornis ,at the amino acid residues 147-167, suggesting the gene is K haemaphysaloides TnI gene.

经同源性比较，该基因预测的氨基酸序列与长角血蜱肌钙蛋白Ⅰ(GenBank，GI：14041807)有很高的同源性(同源性为84.47%)，肌动蛋白结合位点位于147-167氨基酸处，且与长角血蜱肌钙蛋白Ⅰ肌动蛋白结合位点完全相同，表明该基因是镰形扇头蜱肌钙蛋白Ⅰ基因。

To study genetic mutations of methylenetetrahydrofolate reductase C677T and cystathioinine-β-synthase T833C related to homocysteine metabolism in patients with ischemic stroke, the MTHFR gene C677T gene mutation and the CBS T833C gene mutation were detected by PCR-RFLP or ARMS method in 74 patients with ischemic stroke and 83 normal people for control. Results showed that the frequencies of MTHFR genet homogenetic type(2.7%), heterogenetic type(51.4%) and T allele(28.4%) in ischemic group were higher than those in control group(1.2%, 39.8% and 21.1%, respectively).

为研究同型半胱氨酸代谢相关酶亚甲基四氢叶酸还原酶基因C677T和胱硫醚-β合成酶基因T833C位点碱基突变与缺血性脑卒中的关系，对74例缺血性脑卒中患者和83例健康对照者，采用聚合酶链反应-限制性片段长度多态性技术检测MTHFR基因C677T基因型，用扩增阻滞突变体系法检测CBS基因T833C突变。

Result Of the 90 HLER E.faecium,85.6%(77/90) were positive for ermB gene,and 15.6%(14/90) positive for both tnpA and ermB gene. All isolates were negative for mef gene.

结果90株高水平红霉素耐药屎肠球菌中ermB基因阳性率为85.6%(77/90)，mef基因均为阴性，15.6%(14/90)tnpA基因阳性的肠球菌中ermB基因均为阳性。

A new gene structure is proposed: head+body+tail, which allows the program with necessary complexity and putting some learning mechanism into the search process.② A new homeotic gene structure is proposed, it not only can call for subroutines easily, but also can automatically perform programming.③ The concept of different homeotic gene, a multi-cellular structure is proposed. It can be used to describe the complex multi-level programs and to implement the complex subroutine calls.④ An estimation of distribution operator for guiding search is proposed. It fuses statistic learning mechanism into the search process to accelerate the convergent process and improve the quality of solutions.

提出了一种新的基因结构：头＋身＋尾，使计算机自动设计的程序具有必要的复杂性，又便于引入学习机制；②提出了一种新的同源基因结构，它不仅可实现子程序的调用，还具有很强的编程能力；③提出了异族同源基因的概念：一种多细胞结构，它能描述复杂的多层次程序结构，实现可重用程序的复杂调用；④提出了分布估计变异方法，将统计学习机制融入算法，既提高了算法的收敛速度，又提高了解的质量。

RT-PCR analysis showed that GhMADS1 gene expressed in petals, stamens, ovules and fibers, but not in roots, stems, leaves, bracts and sepals. The strongest expression of GhMADS1 gene was detected in petals. But in floral buds of a cotton homeotic mutant (CHV1), whose floral organs are all converted to bract leaf-like organs, the transcript of GhMADS1 gene was not detected.

RT-PCR分析显示，该基因在陆地棉的花瓣、雄蕊、胚珠和纤维中表达，特别是在花瓣中表达量最高，而在根、茎、叶等营养器官和棉花同源异型突变体CHV1（所有花器官均变为苞叶状叶性器官）的变异花蕾中不表达。

RT-PCR analysis showed the GhMASD1 gene expressed in petals, stamens, ovules and fibers, but not in roots,stems, leaves, bracts and sepals. The strongest expression of GhMADS1 gene was detected in petals. But in floral buds of a cotton homeotic mutant (CHV1), whose floral orgens are all converted to bract leaf-like organs, the transcript of GhMADS1 gene was not detected.

RT-PCR分析显示，该基因在陆地棉的花瓣、雄蕊、胚珠和纤维中表达，特别是在花瓣中表达量最高，而在根、茎、叶等营养器官和棉花同源异型突变体CHV1（所有花器官均变为苞叶状叶性器官）的变异花蕾中不表达。

Methods The 26th exon, the 30th exon and the 21st intron of gene GP Ⅱb in 110 individuals were amplified by polymorphism and sequenced to investigate whether there was linkage among the polymorphisms of the gene. Human platelet antigen-3 (HPA-3) gene frequency was detected by Fok 1 enzyme in 147 patients with hematologic diseases, and was compared with that in 110 normal individuals. Forty-four patients who received apheresis platelet transfusion repeatedly were randomly divided into the HPA-3 homotype group and the control group. The antibodies of the platelet were detected after 3 times of platelet transfusion.

聚合酶链反应－单链构型多态性分析检测110例正常人GP Ⅱb基因第26和30外显子(Exon 26， Exon 30)及21内含子(Intron21)基因多态性，进行基因序分析，研究这些基因多态性是否存在连锁关系；应用Fok1酶切法对147例血液病人人类血小板抗原－3(human platelet antigen-3， HPA-3)基因进行分型，并与110例正常人进行比较；将接受单采血小板输注的44例血液病人随机分为HPA-3同型输注组和对照组，血小板输注3次以后检则血小板抗体。

This study intends to investigate the correlation between HBV-GN and HLA-DR allele by testing the frequency of HLA-DR gene in HBV-GN patients, to probe into the effect of immunogenetic factor on the pathogenic mechanism of HBV-GN at the genic level and further explore susceptibility gene and/or resistance gene associated with HBV-GN disease, so as to provide certain experimental basis for diagnosis and treatment of HBV-GN.

本研究拟通过检测HBV-GN患者的HLA-DR的基因频率，对HBV-GN与HLA-DR等位基因相关性进行研究，从基因水平探讨免疫遗传因素在HBV-GN发病机制中的作用，并进一步探索HBV-GN疾病相关的易感基因和/或抗性基因，从而为HBV-GN的诊断和治疗提供一定的实验依据。

Gondii, were cloned by PCR respectively. The PCR products were digested by the corresponding enzymes and ligatd into the intermedial vectors. Finally, the inducible RNAi vector, pBSK-HSP70/5UTR-IntronC-HSP70/3UTR, with the HSP70 gene promotor as a promotor, the intron C sequence ofβ-tubulin gene as intervening sequence, 3UTR sequences of HSP70 gene as transcription stop signals, was constructed successfully, and the results of sequencing were correct. 3The construction of the inherited and inducible RNAi vector system of T. gondii: The fragment of SAG1/5UTR-eGFP-SAG1/3UTR in pBSK-SAG1/GFP vector was cloned into the vector of pBSK-HSP70/5UTR-IntronC-HSP70/3UTR to construct pBSK-GFP-Hairpin vector, then the fragment of GFP-Hairpin in pBSK-GFP-Hairpin vector was cloned into pHANA-0.5 vector.

弓形虫可诱导的反向重复序列RNAi载体的构建：设计引物，通过PCR分别扩增弓形虫HSP70基因5&UTR启动子序列(HSP70／5UTR)、HSP70基因3&UTR序列(HSP70／3UTR)及β-微管蛋白基因内含子C序列，通过酶切连接，构建以弓形虫热休克蛋白HSP70基因启动子进行驱动的，以β-微管蛋白基因内含子C序列作为间隔序列，以HSP70基因3UTR序列作为转录终止信号的反向重复序列RNAi载体pBSK-HSP70／5UTR-IntronC-HSP70／3UTR，序列测定结果正确。

The main component include promoter sequence (5' flank control rigon of goat P - lacto globulin gene of goat),foreign gene,Marker gene and genome of defective retrovirus.

其中主要构建元件包括：启动子序列(山羊β-乳球蛋白基因5'端调控区)；外源基因(人乳铁蛋白基因cDNA)；标记基因；缺陷型逆转录病毒基因组。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。