查询词典 Gene

To investigate the role of gene Gli3 in idiopathic congenital talipes equinovarus, we constructed the Gli3 luciferase reporter gene expression vectors to analyze the promoter activity of the rat gene Gli3. The regulatory element in the promoter region of the rat Gli3 was predicted using P-Match software and further verified by ChIP experiment.

为了探讨人类GLI3基因在单纯性马蹄内翻足(Idiopathic congenital talipes equinovarus， ICTEV)发生时所起的作用，文章构建了大鼠Gli3基因启动子区域荧光素酶报告基因表达载体来分析Gli3基因启动子的活性，并利用P-Match软件预测大鼠Gli3基因启动子区域可能的调控元件，应用ChIP实验加以验证。

Methods: the experimental condition for preparing the gene chips was studied and 7 fragments were screened from GenBank as probes dotted on chips to make HBV genotyping gene chips, many examinatorial experiments were done to evaluate the quality of this gene chip, and to analysis its detection characteristics.

对大量HBV各基因型序列对比分析后，设计7支探针，用芯片点样机点样于经预处理的玻片上制成HBV基因型检测芯片；对芯片检测的实验条件进行研究探索；进行芯片质量性能的考察实验，分析其检测特性。

ERAF17 gene expresses differently between male and female reproductive organs. Its expression is induced by ethylene or ethylene released compounds that can promote femaleness. No evidence directly shows the relationship between ERAF17 gene and femaleness, however. So this work focus on proving the positive correlation between the expression of ERAF17 gene induced by ethylene and development of female flowers,then search for the signal pathway of cucumber female reproductive organ development.

ERAF17基因在黄瓜的雌雄生殖器官的表达不同，可以由乙烯诱导表达，从而推动雌性化，但是它的作用机制却不清楚，并没有明确的证据表明ERAF17基因与雌花的发育有直接关系，所以本论文研究重点在于证明乙烯诱导ERAF17基因的表达对黄瓜雌花的分化有确定的正相关，从而可以探索黄瓜雌性器官发育存在的可能的信号通路，并对应用前景进行评估。

Coli and S.aureus , the experiment showed that recombined gene peptide has the antibacterialactivities.Conclusion The recombined gene antibacterial peptide was expressed successfully in the fusional pTYB vetor and the fusion gene peptide CA(1 ~8-MA(l ~12),CAM has the antibacterial activities to E. coli and S.aurens. While how to frame a more efficient expression system should be studied further , and its other biological activities are still to be tested.

本实验通过基因克隆方法拼接重组基因抗菌肽与融合表达载体pTYB12并在大肠杆菌中进行表达成功，抑菌活性的检测结果表明重抗菌肤的克隆表达与活性初步测定中文摘要组基因抗菌肤CA(1一8卜MA(1一12)，cAM〕对大肠杆菌与金黄色葡萄球菌均有一定的抗菌活性，如何进一步提高抗菌肤的表达量和有关重组基因抗菌肤的生物学活性还有待于进一步研究。

One, the progress of the research of mankind of gene medicines and chemical reagents to oneself gene and gene project will be in will make pharmacy line of business obtains a leap in 10 years henceforth, remedial osteoporosis, early old sex fiber of sex of gawkish, bursa is out of shape the medicaments that waits for difficulty miscellaneous disease will come out, the cure of AIDS also will obtain a breakthrough.

一、基因药品人类对自身基因的研究和基因工程的进展将在今后10年中使制药业取得飞跃，治疗骨质疏松、早老性痴呆、囊性纤维变形等疑难杂症的药物将问世，艾滋病的治疗也将取得突破。

The wheat germin gene was cloned by PCR and a plant expression vector was constructed harboring the germin gene drived by CaMV 35S promoter. Germin gene has been transferred into the tabacco plants.

本研究通过PCR扩增克隆了小麦germin基因，构建了35S启动子驱动的germin基因植物表达载体，经遗传转化获得了转基因烟草植株。

The wheat gernhin gene was cloned by PCR and a plant expression vector was constructed harboring the germin gene drived by CaMV 35S promoter. Germin gene has been transferred into the tabacco plants.

本研究通过PCR扩增克隆了小麦germin基因，构建了35S启动子驱动的germin基因植物表达载体，经遗传转化获得了转基因烟草植株。

The Lyb genes were novel members of Y-box gene family containing a conserved cold-shock domain with two RNA binding motifs (RNP-1 and RNP-2). Moreover, phylogenetic analysis revealed that there was only one type of Y-box gene before the emergence of gnathostomata. By contrast, the Y-box gene were divided into three groups, YB1, YB2, and CSDA during evolution of jawed vertebrates.

此外， 从Swiss-Prot下载了经实验验证过的Y-box蛋白序列数据，结合实验克隆得到的Lyb基因所编码的氨基酸序列构建系统发育树，发现Y-box基因在有颌类出现以前只有一种类型，有颌类出现以后， Y-box基因分化成YB1、YB2和CSDA三种类型。

Phylogenetic analysis based on gyrB gene is consistent with those of 16S rDNA tree and provided a better discrimination within the species of Gordonia compared to 16S rRNA gene. The present study demonstrates that gyrB gene analysis will aid in describing novel species belonging to the genus Gordonia.

本研究利用上述两基因序列相似度初步判定4株本土Gordonia新菌种之分类地位，其中分离株CC-AB07经由生理生化特性及化学组成分析，已确认为本土Gordonia新菌种。

The phylogenetic analysis of Gymnothorax was reported in this paper by being based on DNA sequences of mitochondrial Cytb gene.17 species of Cytb gene full-length sequences were obtained and their length of Cytb gene was 1140bp.All sequences were analysised by using biological software, the phylogenetic tree was constructed by using three kinds of common contribution phylogenetic tree methods and the phylogeny of 17 species of Gymnothorax was deduced.

本论文采用线粒体Cytb基因为分子标记，共获得裸胸鳝属鱼类17种Cytb基因序列全长为1140bp的部分序列，应用分子生物学软件对结果进行分析，使用3种常见的建树方法：NJ法、ME法和MP法重建系统发育树，对裸胸鳝属17种鱼类的系统关系进行研究，为探讨裸胸鳝属鱼类的分子进化，确定它们之间的关系提供分子生物学方面的证据。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。