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CML is cytogenetically marked by the philadelphia chromosome, which originates from a reciprocal translocation between chromosome 9 and 22 and is molecularly marked a chimeric bcr-abl gene, resulting from juxta-positive of the abl proto-oncogene on chromosome 9 with the bcr gene, which is normally located on chromosome 22. The chimeric bcr-abl gene expression an 8. 5kb hybrid mRNA transcript giving rise to a 210-KD fusion protein (P210〓) with increased tyrosine kinase activity. P210〓 plays a key role in the pathogenesis of CML. The continuous cell line K562 was established from the pleural effusion of a 53-year-old female with CML in terminal blast crisis, and was a human erythroleukemia line, contained Ph chromosome.

绝大多数慢粒患者白血病细胞中具有Ph染色体，是由9号染色体长臂3区4带和22号染色体长臂1区1带相互易位形成，即t（9；22），使位于9q〓的c-abl原癌基因在第二外显子的5'端断裂并易位到22 q〓的M-bcr基因第2或第3外显子的3'端，形成异常的bcr-abl嵌合基因，该基因转导出异常的mRNA，编码并翻译出P210蛋白，该蛋白具有很强的酪氨酸激酶活性，使粒细胞发生恶性增殖。K562细胞属于慢粒急变、红白血病细胞株，具有Ph染色体。

The results showed that 17 gene fragments were expressed exclusively,26 gene fragments expressed increasingly,and 20 gene fragments expressed decreasingly in incompatible combination compared with either in compatible combination or in control.

结果表明，非亲和性互作中特有的表达片段7条，较亲和性互作及对照表达明显增强的片段21条，表达明显减弱的18条。

Chronic injury of Hepatic, immunologic response and the oxidized of DNA injury may cause gene mutation, carcinogenic gene;oncogene activated , tumor-suppressing gene devitalized, at last result in carcinogenesis.

本课题从基因及蛋白质两方面检测HDAC6在肝癌组织中的表达并探讨其与肝癌发生发展过程中相关的临床病理因素间的相关性，从而期望能够发现肝癌发病及发展和转移新的因素，为肝癌临床治疗提供理论依据。

Possible reasons for this failure may be that obesity is so physiologically complex that no single gene or even handful of genes is likely to have a dispositive role, that examining gene gene interactions is difficult without large numbers of subjects, and that variations among current candidate genes are not the most important contributors to human adiposity.

其他有明显量的变化的基因很难证明，可能的原因就是肥胖是一个复杂的生理变化过程，单个基因或一部分基因会能起到真正的作用，没有大规模人群实验检测基因相互作用还很困难，而且当前的候选基因变化并不是人类肥胖的主要原因。

The present invention relates to a process of producing a functional immunoglobulin superfamily protein, which has at least one disulphide bond when functional, the process comprising the steps of providing a bacterial cell comprising a gene coding for the protein, the gene is expressible in said cell, cultivating the cell under conditions where the gene is expressed, isolating the protein from the cell without reducing it, and subjecting the isolated protein to a folding treatment.

本发明涉及一种产生功能性免疫球蛋白超家族蛋白的方法，此蛋白质在有功能时具有至少一个二硫键，此方法包括如下步骤：提供一种包含编码此蛋白质的基因且此基因可在其中表达的细菌细胞；在使此基因表达的条件下培养此细胞；从该细胞中分离出此蛋白质而不还原它；以及使分离出的蛋白质经受折叠处理。

The SDS-PAGE electrophoresis indicated that all engineered strains containing different expression plasmid could apparently express a 45 kD band specific for daunorubicin C-14 hydroxylase. The expression level of doxA gene was much higher when the doxA gene was under the control of promoter PermE. However, it was found that fd terminator had few effect on the expression of doxA gene.

SDS-PAGE蛋白电泳实验证明，上述五个菌株都能够表达大小约45 kD的柔红霉素C-14羟化酶蛋白，并发现以在PermE启动子控制下的doxA基因表达量相对较高，而终止子对＠中英文摘要 dd基因表达似乎没有影响。

SMMC-7721 cells transfected with antisense DNMT1 (DNA methyltransferase Ⅰ) gene fragment (SMMC-7721 aDNMT1) was more sensitive to TRAIL than SMMC-7721 cells transfected with sense DNMT1 gene fragment or vectors without any ectogenous gene fragment.

4MTT法和TUNEL法测得的结果显示，肝癌SMMC-7721细胞转入反义DNMT1基因片段后对TRAIL更加敏感。

There were few studies on the causative gene of PCOS in our country. We chose the candidate gene associated with androgen receptor gene to study, considering the endocrinal characteristics such as hyperandrogenism.

本课题针对PCOS常见的高雄激素血症的内分泌特征，选择雄激素受体基因进行研究，旨在探讨AR基因微卫星CAG多态性与PCOS发病及临床特征的关系，寻找PCOS的致病基因，以期为PCOS的预防和治疗提供理论基础。

Homology modeling of 3-D structure of two AChE from L.entomophila were constructed using H.sapiens(1p0i:A) native BuChE structure and Drosophila melanogaster(1d×4:A) native AChE structure as templates,respectively,by SWISS-MODEL.The catalytic triad were found and denoted in the 3-D structure of AChE from L. entomophila referring to T.californica.2.2 Gene cloning ofβ-actin and mRNA expression levels of two AChE genes from L. entomophilaBecause no reference gene has ever been used in Real Time PCR for L.entomophila in GeneBank,a fragment ofβ-actin gene was cloned from L.entomophila(GenBank Accession No.: FJ041117).It consists of 822 bp encoding a protein of 273 amino acids residues.

利用蛋白质结构同源建模工具，分别以人丁酰胆碱酯酶(1p0i：A)和果蝇乙酰胆碱酯酶(1d×4：A)的蛋白晶体结构为模板，对嗜虫书虱2个AChE的三维结构进行同源建模，并在三维结构中发现了AChE的酶解活性位点，证明嗜虫书虱体内也存在2个AChE基因。2.2嗜虫书虱β-actin基因克隆及乙酰胆碱酯酶基因mRNA表达水平研究目前关于嗜虫书虱的分子生物学研究较少，在GenBank中没有可用作内参基因的序列，因此本研究从嗜虫书虱体内克隆获得β—actin基因片段(GenBank登录号：FJ041117)，该片段长度为822 bp，编码273个氨基酸残基，同源性比对分析表明该片段与其它昆虫的β—actin基因具有很高的同源性。

ASR1 is a gene from early auxin-responsive GH3 gene family, and the protein encoded by this gene is overexpressed in the mutant, which results in the following morphological phenotypes including dwarf, epinastic rosette leaves, and restrained lateral root formation, etc. Meanwhile, ASR1 may modify the plant defense signaling molecule salicylic acid in vivo, and therefore asr1 mutant is more susceptible to the infection from the pathogen Pseudomonas syringae.

ASR1属于生长素相关的GH3基因家族，它编码的蛋白质在突变体内过量表达，并造成植株矮化，莲座叶片向下卷曲，侧根发生受到抑制等形态表型的改变，同时，ASR1可能还参与了植物体对抗病信号分子水杨酸的调控或修饰过程，并使asr1突变体对病原菌丁香假单胞杆菌的侵染更敏感。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。