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When the deduced amino acid sequence of the new gene was compared with those of other glutelin genes, they showed about 80%, 70% and 60% between the new gene and GluB-4, GluB-1 or GluB-2, GluA, respectively, suggesting that the new glutelin gene belonged to the subfamily, GluB.

克隆测序和同源性分析比较结果表明，由该基因推导的氨基酸序列与GluB-4相似性近80％，与GluB-1，GluB-2的相似性近70％，而与GluA亚族各谷蛋白的相似性不到60％。

Methods The total RNA of leucocyte was extracted 43 allergic vasculitis patients and 24 normal subjects before and after treatment with Trizol reagent and the integrity and concentrations of HLA-DRw53 gene mRNA were determined by agarose gel electrophoresis and UV spectrophotometer after denatured with 1% formaldehyde, the product was transcribed into complementary retroviral synthetic deoxyribonucleic acid, and sequence-specific primers were designed based on the sequences of second HLA-DRw53 gene exon and housekeeping gene glyceraldehyde phosphate-dehydrogenase cDNA.

利用随机引物逆转录合成互补脱氧核糖核酸，根据HLA-DRw53基因第二外显子序列和管家基因人磷酸甘油醛脱氢酶的cDNA序列设计序列特异性引物。检测43例变应性血管炎患者治疗前后与24名正常人HLA-DRw53基因mRNA表达量的变化。

To analyse hereditary features and and rules of the foreign gene in the trans-Hu-IFN-α-gene carp,PCR testing technology were employed to test the Hu-IFN-α gene's genetic expression in its cross F1 and gynogenesis F1 generation.

为了分析外源基因在转基因鱼F1中的遗传特征和规律，采用PCR检测技术对Hu-IFN-α基因在转基因草鱼及其杂交F1，雌核发育F1中的遗传表达情况进行检测。

To analyse hereditary features and and rules of the foreign gene in the trans-Hu-IFN-α-gene carp, PCR testing technology were employed to test the Hu-IFN-α gene＇s genetic expression in its cross F1 and gynogenesis F1 generation.

文章摘要：为了分析外源基因在转基因鱼F1中的遗传特征和规律，采用PCR检测技术对Hu-IFN-α基因在转基因草鱼及其杂交F1，雌核发育F1中的遗传表达情况进行检测。

Three pairs of primers were designed according to nuc gene of SA, hly gene of LM and hemolysin gene of BC, and then the single PCR was performed to detect specificity and sensitivity.

根据SA的nuc基因、LM的hly基因和BC的hemolysin基因，设计合成三对特异性引物，然后分别进行单基因PCR反应的特异性和灵敏性验证。

Results The p53 gene mutation rate of colorectal cancer was 50%. The rate of apoptosis of tumor cells having mutation of p53 gene (22.11%) was much lower than that having no p53 mutation (40.57%, P＜0.05). Heteroploid cells had been found in 10/13 tumor samples with p53 gene mutation and 6/13 samples without p53 mutation (P＜0.05). The rate of apoptosis had no difference between the heteroploid cases and diploid cases.

结果 大肠癌标本p53基因突变率为50%；其中突变组肿瘤细胞凋亡率(22.11%)明显低于未突变组细胞凋亡率(40.57%)，(P＜0.05)；突变组异倍体细胞百分率(占76.9%)明显高于未突变组(占46.2%)，(P＜0.05)；异倍体肿瘤细胞凋亡率为34.0%，二倍体肿瘤细胞凋亡率为40.7%，二者差异无显著性(P＞0.05)。

Caghira gene is expressed in the unfertilized eggs and all stages of embryogenesis and the quantity of Caghira gene expression didn\'t changeobviously, which could be inferred that hira gene had an effect on gynogenetic development of gibel carp.

在银鲫的未受精卵中和胚胎发育不同阶段均可以检测到少量的转录产物，表达量未检测到明显变化，表明hira基因可能在银鲫雌核发育过程中发挥一定作用。

Caghira gene is expressed in the unfertilized eggs and all stages of embryogenesis and the quantity of Caghira gene expression didnt changeobviously, which could be inferred that hira gene had an effect on gynogenetic development of gibel carp.

在银鲫的未受精卵中和胚胎发育不同阶段均可以检测到少量的转录产物，表达量未检测到明显变化，表明hira基因可能在银鲫雌核发育过程中发挥一定作用。

The result of WISH showed that the trascription of hira gene distributed widely in the early stages of embryos development and centralized to head, heart and the end of tail. Hira gene might affect the development of brain and heart. The expression characterization of colored crucian carp Cahira gene in different tissues and different stages of embryogenesis were also investigated by RT-PCR.

胚胎原位杂交显示，Hira基因的转录本在银鲫胚胎早期广泛分布，而在胚胎后期主要集中于头部、心脏及尾芽尖部，说明也对其脑部和心脏的发育有影响。

It is suggests that Doc -1R gene has particular importance in maintaining the function of the tissues and Doc-1R gene perhaps is a house-keeping gene.

小鼠Doc-1R基因的mRNA在各组织均为阳性表达，因此提示，Doc-1R基因可能是一种对维持正常细胞功能具有重要意义的管家基因。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。