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The Omp25 gene of wapiti's Brucella abortus was amplified by RT-PCR and sequenced, Then the interesting gene was subcloned into pET28a for expression. The results showed that the full length of Omp25 gene was 642bp, which encoded 213 amino acids.

运用RT-PCR技术从马鹿布氏杆菌分离株中扩增出Omp25基因，将其克隆入pMD18-T中进行核苷酸序列测定和遗传变异分析，并将目的基因亚克隆到大肠杆菌表达载体pET28a中诱导表达。

In this paper, major agronomic traits and grain quality traits affecting rice yield and grain quality were investigated in seven aromatic rice maintainer lines bred in Sichuan and one variety introduced from America. It was aimed at estimating the numbers of gene controlling aroma, the allelism of aroma genes, location of aroma gene on the chromosome, and molecular tagging of aroma gene.

本研究利用四川新育成的七个香稻保持系(D香1B、D香2B、内香2B、内香4B、绵香2B、绵香3B、宜香1B)、一个香稻引进改良品种(泸香90)和一个非香型保持系为材料，研究了香稻保持系主要农艺性状和品质性状对品质、产量的影响，并且通过杂交群体构建对香稻香味性状进行了遗传分析。

The light and heavy chain variable region gene of the monoclonal antibody had been cloned and sequenced. VH region gene consisted of 350 bp, and VL region gene consisted of 321 bp encoding 107 amine acid residues .

采用分子生物学方法，对抗RB单克隆抗体的轻重链可变区基因进行克隆及序列分析，重链约350bp，轻链为321bp，编码107氨基酸。

Y39 should carry a novel resistance gene. A dominant major gene conferring resistance to powdery mildew of wheat was identified in a BC〓F〓 population derived from a cross between amphidiploid Am4 and wheat cv. Bainong3217. The gene originated in T.

本研究的主要目的是对从小麦野生近缘种转移到普通小麦中的抗小麦白粉病基因进行鉴定、微卫星标记和作图，并用鉴定的微卫星标记对一些高代品系所携带的抗白粉病基因进行检测。

The α-α gene, nMT gene and nα-α gene were transformed to Anabaena sp.

基因，nMT基因和nα-α基因分别被转入蓝藻鱼腥藻Anabaena sp。

A pair of primers was designed according to the coat protein gene of Arabis mosaic viru and used to amplify a 990 bp partial gene of the cp by reverse transcription-polymerase chain reaction, the 990 bp partial gene was cloned into the pEGX-9p-1, a prokaryotic expression vector fused with GST. The recombinant vector was transformed into E. coli BL21. The fusion protein was successfully expressed in E. coli BL21 induced with the IPTG at 37℃.

依据报道的ArMV外壳蛋白基因(coat Protein， Cp)序列设计引物，通过RT-PCR 扩增得到长约990bp的ArMV cp基因亲水基团部分片段，将目的片断克隆到原核表达载体pEGX-6p-1中，构建ArMV cp基因与GST蛋白融合表达载体pEGX-cp，重组载体化大肠杆菌BL21，经IPITG诱导后，融合蛋白GST-cp得到了特异表达。

Indica variety ZYQ8 is an important genetic resource resistant to rice blast disease in rice breeding in North China.in this study,resistance identification of the populations of F1,F2,DH and B1F1of the crosses of ZYQ8×Jingxi17 (JX17) and ZYQ8×Lijiangxintuanheigu was carried out by using a Chinese differential strainZH10-8-14 from North China and a Japanese differential strain Ken54-04 from Japan and the results demonstrated that ZYQ8 had one dominant major gene Pi-zh which had been reported by Zhu L.H., et al., The results from experiments in which the F2 populations of ZYQ8×nine different varieties with known resistant genes were inoculated with strain Ken54-04 indicated that the gene Pi-zh was a new gene and non-allelic to nine known gene loci,namely Pi-i,Pi-km,Pi-z,Pi-ta,Pi-ta2,Pi-zt,Pi-kp,Pi-b and Pi-t.

籼稻品种窄叶青8号是我国北方稻区水稻育种上重要的稻瘟病抗源之一。本文利用我国北方稻区的代表菌系中10-8-14和日本的代表菌系研54-04，对窄叶青8号与感病品种京系17号和丽江新团黑谷的杂交F1、F2、DH和B1F1群体进行抗病性鉴定。根据抗病性的分离，确认窄叶青8号的抗性由1对显性主效基因，即朱立煌等报道的Pi-zh基因控制。利用菌系研54-04接种窄叶青8号与9个具有已知抗病基因的鉴别品种杂交的F2群体，各群体都表现二基因的独立遗传，证明Pi-zh基因与Pi-i、Pi-km|、Pi-z、Pi-ta、Pi-taz、Pi-zt、Pi-kp、Pi-b和Pi-t等9个已知抗病基因间存在非等位关系，是新的抗稻瘟病基因。

The inhibinβA gene was studied as a candidate gene for the prolificacy of Jining Grey goats. According to the sequence of bovine INHBA gene, four pairs of primers were designed to detect single nucleotide polymorphisms of exon 1 and exon 2 of INHBA gene in both high fecundity breed and low fecundity breeds (Inner Mongolia Cashmere goat and Angora goat) by PCR-SSCP.

采用PCR-SSCP技术检测抑制素βA亚基基因（inhibin βA，INHBA）的编码区在高繁殖力山羊品种和低繁殖力山羊品种（内蒙古绒山羊、安哥拉山羊）中的单核苷酸多态性，同时研究该基因对济宁青山羊高繁殖力的影响。

The follicle-stimulating hormone beta-subunit gene was studied as a candidate gene for the prolificacy in Jining Grey goats. According to the sequence of ovine FSHβ gene, nine pairs of primers were designed to detect single nucleotide polymorphisms of 5' regulatory region, exon 1 and exon 2 of FSHβ gene in both high fecundity breed and low fecundity breeds (Liaoning Cashmere goat, Boer goat and Angora goat) by PCR-SSCP.

采用PCR-SSCP技术检测促卵泡素β亚基(follicle-stimulating hormone β， FSHβ)基因5'调控区、外显子1和外显子2在高繁殖力山羊品种和低繁殖力山羊品种（辽宁绒山羊、波尔山羊、安哥拉山羊）中的单核苷酸多态性，同时研究该基因对济宁青山羊高繁殖力的影响。

The experimental results showed that all characters accorded with the addi tiv e-dominant-epistatic model, the additive gene effect and dominant gene effect pl ayed an important role in most characters of 6 crosses, epistatic gene effects w ere significant in all crosses for the 6 investigated traits, additive generic g ene-effects were more important on the spikelets per panicle and spike lets density than its non-additive generic gene-effects (［h］+［j］+［l］), b ut the l atter were relatively more crucial on the days to heading and panicle length in the two tested lines.

结果表明：六个性状都符合加性-显性-上位性模型，加性效应对两个多小穗小麦品系各性状的遗传有重要作用，部分性状存在显著的显性效应，不同类型上位性效应对各性状有不同程度的影响；各性状之间各类基因效应平方和百分比差异较大，不同类型上位性基因效应平方和百分比大小因被测系、组合而异。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。