查询词典 Gene

Known differential expression gene: In the 46 points of known differential expression gene, there were down - regulate gene expression 41 points and up- regulate gene expression 5 points; twenty - eight genes related to hypoxia/reoxygenation had not been reported and 18 genes had been reported.

已知差异表达基因：在46个已知差异表达基因中，表达下调的基因有41个，表达上调的基因有5个，未见报道的与缺氧/复氧处理相关基因23个，已报道的有18个。

The interaction between M. grisea and rice is based on the gene-for-gene hypothesis and the defense responses are often activated by the action of the pathogen avirulence gene and the host resistance gene.

稻瘟病菌与水稻的互作符合经典的基因对基因学说，二者的不亲和互作是无毒基因与抗病基因相互作用的结果。

The interaction between M.grisea and rice is based on the gene-for-gene hypothesis and the defense responses are often activated by the action of the pathogen avirulence gene and the host resistance gene.

由Magnaporthegrisea(无性世代为Pyricularia grisea或Pyculariaoryzae)引起的稻瘟病是世界农业生产上危害很严重的病害之一[1]。

The total RNA was abstracted from the larvae of Boophilus microplus, and a 1982bp Bm86 gene was amplified by RT-PCR. The target gene was subcloned into T vector. The sequencing showed that the nucleotide sequence of the cloned Bm86 gene shared 97.4% homology with the data published in and this fragment contained the complete open reading frame of Bm86 gene.

为了克隆微小牛蜱Bm86 基因及构建该基因的表达载体，以微小牛蜱饥饿幼蜱的破解物提取的总RNA为模板，参照已发表的微小牛蜱Bm86基因的核苷酸序列，设计了1对引物，采用RT-PCR技术获得微小牛蜱Bm86基因；将Bm86基因克隆入载体，并进行序列分析，结果证明，克隆的Bm86基因序列与GenBank上登录的Bm86基因序列的同源性达97.4%，而且该序列包含完整的开放阅读框。

In order to direct the construction of plant germplasms by elucidating the relatives among plants at the level of gene, CYP86MF gene analogues from 11 species of 6 genera in Cuciferae were respectively obtained by PCR strategy using gene specific primers designed from conserved regions of CYP86MF gene reported. Sequence comparisonindicated that the similarities among the genes at nucleotide level were over 80%, and the similarities at amino acid level remained above 70%. The differences between the genes at nucleotide and amino acid level between species were 1.0%~ 5.7% and 2.6%~ 7.3% respectively, while those between genera 5.6%~ 22.5% and 7.3%~ 31.2%, respectively. Phylogenetic analysis showed that Brassica was closely related to Raphanus, followed by Rorippa Scop, Arabidopsis Heynh, Capsella Medic orderly, most distantly related to Orychophrogmus.

为了从分子水平阐明十字花科植物间的亲缘进化关系，给植物种质资源的创建提供理论依据，试验根据课题组已报道的CYP86MF基因编码的氨基酸保守区域设计特异引物，运用PCR技术分别从十字花科6个属11个物种中分离克隆到了CYP86MF基因的同源序列，经比较分析，结果表明：这些同源序列的相似性达80%以上，所推导的氨基酸序列相似性达70%以上，且两者种间差异分别为1.0%~ 5.7%和2.6%~ 7.3%，属间差异分别是5.6%~ 22.5%和7.3%~ 31.2%；由氨基酸序列构建的分子系统树可知，在亲缘进化关系上芸薹属与萝卜属较近，其他依次为蔊菜属、拟南芥属、荠菜属，而与诸葛菜属最远。

Small interference RNA homolog to silenced gene in sequenceis indued into cell to clearage the mRNA of target gene. Many studies havedemonstrated that RNAi is an effective tool for gene diagnosis and gene therapy.

我们根据文献报道选择EZH2基因cDNA的461～481碱基为靶序列，构建EZH2-siRNA表达质粒，并研究其对人膀胱癌EJ细胞EZH2基因表达及生长抑制作用。

Objective: To colone SOD gene, construct combinant of retrovirus SOD gene, make a basis of gene transfection and over-expression of gene therapy.

目的 通过克隆SOD基因，构建逆转录病毒-SOD真核表达载体转染复合体，为基因转染及SOD的过表达提供基础，为将来的基因治疗进行准备，也为深入研究SOD功能及SOD的开发创造条件。

Long-distance PCR was used to amplify the β-actin gene for black carp based on the β-actin gene sequences of Cyprinidae. Construct of EGFP expression system containing 5'UTR of β-actin gene for black carp was transferred into zygotes of mud loach and COS-7 cells and the results showed that 5'UTR of β-actin gene for black carp could be used as the promoter of the"all-fish"transgene.

基于鲤科鱼类Beta-肌动蛋白基因序列，采用长片段PCR技术，克隆了青鱼β-actin基因DNA片段，并构建了青鱼β-actin基因5'启动调控区绿色荧光蛋白表达载体，通过显微注射泥鳅胚胎和COS-7细胞转染实验，证明了所克隆的青鱼β-actin基因5'启动调控区具有启动调控功能，并用之来构建&全鱼&基因的启动子部分。

Five plasmids for the expression of doxA gene, which were designated as pYG57, pYG502, pYG503, pYG505 and pYG506 were constructed so that the cloned doxA gene were under the control of either promoter of melanin gene or erythromycin resistant gene or their tandem promoters, and a fd terminator downstream.

构建了五个doxA基因链霉菌表达质粒pYG57、pYG502、pYG503、pYG505和pYG506，使得doxA基因在上游的Pmel启动子、红霉素抗性基因启动子或者两者的串联启动子，以及下游的fd终止子控制之下。

We selected mecA gene and femA factor asβ-lactam antibiotic-resistant genes, ermA, ermC, msrAgene as macrolides-lincosamids antibiotic-resistant genes, norA, grlA, gyrA, gene as Quinolonesantibiotic-resistant genes, and tetM gene as Tetracycline antibiotic-resistant genes on the basis of the latestresearch. We designed primers with GenBank enunciable gene sequence, cloned the drug resistantgenes, and sequenced these genes.

根据国内外最新文献报道，筛选出对β-内酰胺类抗生素耐药的mecA基因及辅助因子femA；对大环内酯类及林可霉素类抗生素耐药的ermA、ermC、msrA基因，对喹诺酮类抗菌药物耐药的norA、grlA和gyrA基因及对四环素类抗生素耐药的tetM基因作为拟选的主要耐药基因，分别设计PCR扩增的特异性引物，进行克隆。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。