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M treatment materials, and also tartaric acid and no maleic acid detected in G. v treatment materials. Inoculation also increased the content of organic acid secretion (increased by 19.80～56.87 mg/kg) of trifoliate root, and the application of AlPO4 greatly increased organic acid content (increased by 20.06～21.84 mg/kg); uninoculated plant root secreted a bit of organic acid; Mycorrhizal poncirus trifoliata seedlings excreted malic acid (42.87%), citric acid (39.22%) and oxalic acid (12.06%).

接种AM真菌的根系分泌的有机酸种类与对照有所不同，未接种处理枳分泌的有机酸有草酸、苹果酸、乳酸、乙酸、顺丁烯二酸和柠檬酸等6种，而接种G.m的则检测到草酸、酒石酸、苹果酸、乳酸、乙酸、柠檬酸、丁二酸等7种，G.v处理的检测到酒石酸，接种处理均未检测到顺丁烯二酸；接种丛枝菌根真菌增加了枳根系分泌有机酸的量比未接种处理增加19.80～56.87 mg/kg，且施用AlPO4后有机酸含量显著增加(增加20.06～21.84 mg/kg；未接种植株根系仅分泌少量有机酸；接种植株根系分泌的有机酸以苹果酸(42.87%)、柠檬酸(39.22%)和草酸(12.06%)为主。

This paper studied the conditions of protoplast preparation of Oenococcus oeni,including the concentration of preculture and lysozyme,lytic time,hypha culture time,etc.As a result,hypha culture time 20 h,penicillinG Na 0.5 μg/mL,lysozyme 1 mg/mL,lytic time 30 min,SMM as osmotic pressure stabilizer,ATB culture medium of regeneration culture mediu...

结果表明，酒酒球菌SD-2a原生质体制备的最佳条件为：菌龄20 h，青霉素G钠质量浓度0.5μg/mL，酶质量浓度1mg/mL，酶解时间30 min，渗透压稳定剂采用SMM缓冲液，再生培养基采用添加0.7 mol/LKCl的ATB培养基，再生培养时采用双层培养基厌氧培养，可以保证较好的形成率和再生率。

To the secondinstar larvae, by dropping on the pronotum and toxicant membrane, the LD50 was 0.015μg/larva and 1.41g/cm^2 at 96h after treatment, respectively. From the second instar, silkworms were fed continuously on mulberry leaves immersed of triazophos solution of different concentration, the LC50 at 24 HAT, 48 HAT, and at the beginning of the third, fourth and fifth instar was 2.38, 1.54, 0.97, 0.74 and 0.53mg/L, respectively.

结果表明，采用点滴法与药膜法，25℃条件下药剂处理后96h，三唑磷对家蚕2龄幼虫的接触LD50值分别为0.015μg/头与1.41μg/平方公分。2龄起蚕开始，于25℃条件下用三唑磷处理的桑叶饲养，在药剂处理后24、48h以及3龄起、4龄起、5龄起，三唑磷对家蚕的LC50值分别为2.38、1.54、0.97、0.74、0.53mg/L。

Results Protocatechuic acid has linear correlation between 0.103 μg and 0.7725 μg,the results also have shown the recovery rate was 97.76%,RSD 1.01%.

结果 原儿茶酸在0.103~0.7725 μg范围内呈良好的线性关系；平均回收率为97.76%，RSD=1.01%。

Results Protocatechuic acid has better linear correlation between 0.103 μg and 0.7725 μg,the results also have shown the recovery rate was 97.76%,RSD 1.01%.

结果 原儿茶酸在0.103~0.7725 μg范围内呈良好的线性关系；平均回收率为97.76%，RSD=1.01%。

Results Protocatechuic acid has better linear correlation between 0.103 μg and 0.7725 μg,the results also have shown the recovery rate was 97.76%,RSD 1.01%.

效果 原儿茶酸在0.103~0.7725 μg领域内呈良好的线关连；均匀接纳率为97.76%，RSD=1.01%。

Pyogenous meningitis; TM. tuberculous meningitis and 2.VME. Viral meningitis or encephlitis. The levels of TNFα were determined by RIA; The levels of G-CSF and SIL-2R were determined by ELISA.

TNFα用放免法，G-CSF和SIL-2R用ELISA法，检测25例PM、19例TM、22例VME脑脊液中 TNFα、G-CSF、IL-2R的含量，并与对照组对照。

The formation of secondary phase in the non-equilibrium peritectic reaction was observed by precise transmission electron microscopy analysis. The microstructural evolution and phase selection regularities under different solidification velocities were constructed from the laser resolidification experiments. A new phenomenon involving two-phase coupled growth was firstly detected in the rapidly solidified Ti-Al peritectic alloy. The Ti-45Al-2Cr-2Nb alloy with fully lamellar structure ( a 2+ g ) was taken as an object to investigate the possible preparation of bulky metastable g phase materials based on both adjusting the competing nucleation relation and changing the growth kinetics condition.

首先通过过冷合金微观组织分析界定了各包晶合金熔体中各竞争相以初生相出现的过冷度区间；结合高温区的相图计算，从各相形核孕育时间角度揭示了过冷熔体中亚稳相与稳定相的竞争形核关系；通过精细微观组织分析，阐明了远离平衡时包晶反应中次生相的形成机理；在激光重凝快速凝固实验中建立了不同凝固速度下合金的组织与相形成规律，并对包晶合金中两相共生生长行为进行了深入分析；最后选取初生相为 a 的全层片 Ti-45Al-2Cr-2Nb 合金，从调整各相竞争形核关系和改变晶体生长动力学条件两个方面探索了块状亚稳 g 相材料的制备和控制。

The trace elements and flavonoids were analyzed by ICP-AES and AlCl3 colorimetry, Results show that the content ranged from 48.0-526.8μg/kg and there were abundant zinc in Hibiscus sabdariffa and Tribute Chrysanthemum.

实验测得微量元素中有益微量元素锌含量范围为48.0-526.8μg/kg，玫瑰茄和贡菊含有丰富的锌；茶花、玫瑰茄含有丰富的锰等；而毒性微量元素铅的含量范围是1077.1～16157.8μg/kg，都未超标。

Stachydrine hydrochloride had a good linearity in the range of 4.0~12.0 μg, r=0.9997, and the average recovery was 99.87%, RSD=1.67%.

盐酸水苏碱在4.0μg~12.0μg范围内线性关系良好，相关系数r=0.9997，平均回收率为99.87%，RSD为1.67%。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。