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METHODS: The bile was treated with 3 different methods, including the 2D-Clean-up kit group: the bile sample was dissolved with lysis buffer (contains: 7 mol/L carbamide, 2 mol/L thiourea, ammonium persulfate with mass concentration of 0.4 g/L, 65 mmol/L dithiothreitol, carrier ampholytes with mass concentration of 0.2 g/L).

将标本分别按3种不同方法进行处理：①2D-Clean-up试剂盒处理胆汁标本：用裂解液，7 mol/L尿素，2 mol/L硫脲，质量浓度为0.4 g/L的过硫酸铵，65 mmol/L二硫苏糖醇，质量浓度为0.2 g/L的载体两性电解质溶解沉淀过夜。

OBJECTIVE:To establish the RP-HPLC method for determination of methyl hydroxybenzoate and ethyl hydroxybenzoate in oral liquid preparations METHODS:The Nova-Park C18 column(3 9mm×150mm,5μm)was used The mobile phase consisted of acetonitrile-water(25∶75)at a flow rate of 1ml/min,the detection wavelength was set at 254nm and the sensitivity was set at 0 01 AUFS RESULTS:The linear ranges of methyl hydroxybenzoate and ethyl hydroxybenzoate were 0 4 412～13 2 360μg/ml(r=0 9 999) and 0 6 672～20 0 160μg/m...

目的 ：建立内服液体制剂中羟苯甲酯和羟苯乙酯的反相高效液相色谱测定方法。方法：色谱柱：Nova -ParkC18 柱(3 9mm×150mm ，5μm)；流动相：乙腈-水(25∶75)；流速：1ml/min ；检测波长：254nm ；灵敏度：0 01AUFS。结果：羟苯甲酯浓度在0 4412～13 2360μg/ml范围内线性关系良好(r=0 9999)；羟苯乙酯浓度在0 6672～20 0160μg/ml范围内线性关系良好(r=0 9998)。结论：该方法简便、快速、准确，可用于医院制剂中防腐剂的检测。

Corresponding RSDs were 3. 2 %～4. 5 % and the detection limit of carbofuran was 0. 1μg/ mL、the detection limit of carbaryl was 0. 03μg/ mL.

回收率为84.5%～103.2%；RSD为3.2%～4.5%；多菌灵的农药检测限为0.1μg/mL、甲萘威的农药检测限为0.03μg/mL。

Results show that maximum activities of LiP and Lac reach 11.3 u/g at the 12th day and 0.0992 u/g at the 15th day, rapidly degraded during 10～20 d, and the total loss rate of dry corn stalk is 18.94% after cultivation for 25d. The relative contents of celluloses and hemicellulose are decreased from 27.1% to 13.1% and from 20.3% to 11.9%, respectively after enzymolysis for 6 d.

黄孢原毛平革菌培养12 d时木素过氧化物酶酶活达到最大值11.3 u/g，15d时漆酶酶活达到最大值0.0992 u/g，秸秆降解集中在10～20 d，第25d时菌解结束，干基总损失率为18.94%；再经过6 d的酶解，纤维素、半纤维素的相对含量分别从27.1%、20.3%下降到13.1%、11.9%。

The experiment lasted 6 weeks and the chicklings ingested feed and drank water freely. Group 1 was control group and groups 2, 3 and 4 were experiment groups, which were fed on the basic diet with additives of 220 mg/kg APS, 4×10^7 cfu/g PM and 220 mg/kg APS + 4×10^7 cfu/g PM.

试鸡按体重随机分为4个组，1组为对照组，饲喂玉米－豆粕型基础饲粮；2~4组为试验组，饲粮是在基础日根基础上分别添加黄茂多糖(220mg/kg)、益生菌剂(4×10^7cfu/g)以及&黄芪多糖(220mg/kg)+益生菌剂(4×10^7cfu/g)&。

An expression construct specified in mammary gland with double cistrons of human G-CSF gene and IRES-EGFP gene under control of ovine beta-lactoglobulin gene flanking sequence,has been constructed in two parts (named fragment I and Fragemnt II)that share an overlapping region of 2.2 kb sequence.Two sites of loxp and lox2272 for homologous recombination were inserted into both flanking regions of G-CSF.The lengths of fragment I and Fragment II are 5.9kb and 5.6 kb,respectively.The whole length of the expression vector (β-LG-hG-CSF-IRES-EGFP) is 9.3 kb.

以绵羊β乳球蛋白基因（β-Lactoglobulin，β-LG）为转基因表达框架，将人G-CSF(Human Granulocyte Colony Stimulating Factor， hG-CSF)基因与报告基因-增强型绿色荧光蛋白(Enhanced Green Fluorescenct Protein， EGFP)基因作为双表达单元拼接到β-LG基因的第一外显子处，并在G-CSF基因两侧引入同源重组位点loxP、lox2272，将打靶基因表达构件β-LG-hG-CSF-IRES-EGFP(总长9.3 kb)分为两段进行构建，片段Ⅰ（长：5.9kb）与片段Ⅱ（长5.6 kb）两段重叠部分为2.2 kb。

And the solution was used for waveguide analysis to G.655 fiber with three claddings and G.656 fiber with five claddings, and the result agrees well with the values by experimental measurement.

该解析法分别被用于三包层G.655光纤和五包层G.656光纤的波导分析，分析结果和实测值具有极好的一致性。

The quantitation was performed with deuterated N-nitrosodiethanolamine as internal standard. The limit of quantitation for N-nitrosodiethanolamine was 50 μg/kg. The mean recoveries were 89.1%~98.2% at the spiked levels of 50~250 μg/kg, with the intra-day precision less than 9% and the inter-day precision less than 11%.

NDELA的方法定量限为50 μg/kg；在50-250 μg/kg的3个添加水平范围内的平均回收率为89.1%～98.2%，日内精密度均小于9%，日间精密度均小于11%。

Qualitative and quantitative analyses were carried out for the analyte under the multiple reaction monitoring mode after the chromatographic separation on a Waters Atlantis T3 column (150 mm×2.1 mm, 3 μm). The quantitation was performed with deuterated N-nitrosodiethanolamine as internal standard. The limit of quantitation for N-nitrosodiethanolamine was 50 μg/kg. The mean recoveries were 89.1%-98.2% at the spiked levels of 50-250 μg/kg, with the intra-day precision less than 9% and the inter-day precision less than 11%.

NDELA经waters Atlantis T3色谱柱(150mm×2.1mm， 3μm)分离后在多反应监测模式下进行串联质谱定性及定量分析，以d8-NDELA为内标定量NDELA的方法定量限为50μg/kg；在50~250μg/kg的3个添加水平范围内的平均回收率为89.1%~98.2%，日内精密度均小于9%，日间精密度均小于11%。

The results showed that the spatial distribution range of soil fertility in Qinling forest area on south slope was soil pH volue 5.97～6.89,organic matter 6.6～50.1 g·kg~-1, total N 0.5～3.8 g·kg~-1 ,available N 33.6～257.3 mg·kg~-1,available P 0.81～5.8 mg·kg~-1,available K 38.9～262.4 mg·kg~-1,clay particle content 11.3%～18.1%,capacity of exchangeable base 68.5～310.2 mmol·kg~-1 respectively; The spatial variation degree of available P, organic matter, and total N was big,whereas clay particle content and active acid pH value was small;the spatial variation degree of soil pH value, organic matter, available P, clay particle content, and capacity of exchangeable base decreased with the deep of soil,whereas soil total N、available N, and available K increased.

结果表明，秦岭南坡土壤肥力指标在整个土壤剖面空间分异范围分别为：土壤pH值5.97～6.89，有机质含量6.6～50.1 g·kg-1，土壤全N含量0.5～3.8 g·kg-1，碱解N含量33.6～257.3 mg·kg-1，速效P含量0.81～5.8 mg·kg-1，速效K含量38.9～262.4 mg·kg-1，粘粒含量11.3%～18.1%，阳离子代换量68.5～310.2 mmol·kg-1；其空间分异程度以速效P、有机质和土壤全N含量较大，土壤粘粒含量和土壤pH值较小；随着土层的加深，土壤pH值、有机质、速效P、土壤粘粒含量和阳离子代换量空间分异程度减小，而土壤全N、碱解N和速效K含量空间分异程度增大。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。