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Methods Ovarian GC DNA fragments of both atretic and developing follicles before and after estrogen (1 μg/ml), androgen (1 μg/ml) treatments were analysed by agarose gel electrophoresis. The expression of bcl-2 mRNA was also determined by northern blotting in GC of developing follicle after sex steroids addition. Results Internucleosomal DNA cleavage occurred in granulosa cells in atretic follicles and after androgen treatment.

应用细胞培养、选择性DNA抽提和琼脂糖凝胶电泳技术分析闭锁卵泡和发育中卵泡颗粒细胞的凋亡状况，及雌激素(1 μg/ml)、雄激素(1 μg/ml)对体外培养发育中的卵泡颗粒细胞凋亡的作用；用Northern印迹杂交技术检测经雌、雄激素刺激后发育中的卵泡颗粒细胞bcl-2基因mRNA表达的变化。

In general, the following technical achievements could be concluded: 1. A sensitive and selective liquid chromatography-tandem mass spectrometric method has been developed to determine trace para red、Sudan I～IV、Sudan red B、Sudan red G、Sudan red 7B、Sudan Black B、Methyl Yellow、Toluidine Red、Fast Garnet GBC Base、Auramine、Oil Orange SS and Sudan Orange G in food, respectively.

详细分析研究了食品中微量的对位红、苏丹红Ⅰ-Ⅳ、Sudan red B、Sudan red G、Sudan red 7B、Sudan Black B、Methyl Yellow、Toluidine Red和Fast Garnet GBC Base7种苏丹红类染料、Auramine和Oil Orange SS染料、以及Sudan Orange G染料的快速、灵敏的液质测定方法。

Isolations of the s.aureus insoluble peptidoglycan:(1) the s.aureus insoluble peptidoglycan was gained by cold, hot and hypersound affection repetat of s.aureus(ATCC25923);(2) the s.aureus insoluble peptidoglycan was gained by 10% trichloracetic acid, 5% trichloracetic acid,NH4HCO3 buffer with trypsin digestion of s.aureus(ATCC25923) in order. 2. Isolations of the s.aureus soluble peptidoglycan and quality control:(1) the s.aureus soluble peptidoglycan was released by short dose benzylpenicillin sodium and purified by sephadex G-100.(2) The quality control in the process of isolation: the soluble peptidoglycan was detected by SLP reagent kit; the LPS was detected by kinetic turbidimetric limulus test;and the protein was detected by improved LOWRY reagent kit.

金黄色葡萄球菌可溶性肽聚糖的分离提取及其质量控制(1)采用青霉素钠诱导法和葡聚糖G-100(Sephadex G-100)凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖；(2)金黄色葡萄球菌可溶性肽聚糖提取过程中的质量控制：采用SLP试剂盒对提取物定性检测，应用动态浊度法鲎试验测定提取物中内毒素含量，采用改良LOWRY试剂检测提取物中蛋白的含量；(3)采用青霉素诱导和Sephadex G-100凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖的条件：青霉素使用量、加入青霉素后孵育时间和菌液浓度等优化选择。

The optimal measuring conditions were investigated. The results indicated that the catalytic reaction to Pt and bromocresol purple was the first-order reaction. The apparent activation energy was 217.4kJ/mol and the maximum absorption wavelength of fading reaction was at 434 nm. The linear range of Pt was 0.16-4.0μg/L and the detection limit was 2.5×10^(-11)g/mL. Most of common ions had no interference with the determination, while ten multiples of Ru, Rh and Os had interference with the determination of Pt. Therefore.

选择了最佳测定条件，实验结果表明，催化反应对铂和溴甲酚紫为一级反应，反应的表观活化能为217.4kJ/mol，褪色反应的最大吸收波长为434nm，方法测定R哪的线性范围为0.16~4.0μg／L，检出限为2.5×10^(-11)g/mL，大多数常见离子不干扰测定，10倍以上的Ru，Rh和Os对测定有干扰，可在测定前分离除去。

Color reaction was performed in organic solution-salt solution biphasic system. The organic solution was 1,2-Dichloroethane-Isoamyl alcohol mixture (V/V=96:4) added Bromophenol blue (per 100 mL added 0.05 g). The salt solution was 55%K2HPO4 aqueous solution added Na3CO3 (per 100 mL added 14 g), under the temperature of color reaction between 12℃ to 25℃.

显色反应在有机溶液－盐溶液两相体系中进行，有机溶液为每100 mL 1，2-二氯乙烷－异戊醇混合溶液（体积比96：4）加入0.05 g溴酚蓝，12~25℃显色反应条件下，盐溶液为每100 mL 55%K2 HPO4水溶液加入14 g Na2CO3。

Methods Rat calvaria osteoblasts were cultured in the presence of 10 μg/mL-500 μg/mL nHA or nAg-HA/TiO2 for 2 h, 6 h, 8 h, 24 h, 72 h and 120 h. Cell proliferation, vitality and ultrastructure were tested using cell counting chamber, MTT assay, and transmission electron microscope.

将成骨细胞分别在添加了不同浓度(10μg/mL~500μg/mL)的nHA或nAg-HA/TiO2的培养基中培养2、6、8、24、72及120h，通过透射电镜观察、细胞计数及MTT法检测细胞超微结构、细胞增殖及线粒体活性，并计算单位细胞呼吸率。

The results demonstrated that the fractional conversion and reaction rate were rapidly increased in the initial curing reaction. The higher the curing temperature, the higher the fractional conversion and reaction rate was. With increasing of the curing temperature and time, the fractional conversion and reaction rate decreased and became constantly. The initial curing reaction was a first order reaction. The apparent activation energy and frequency factor of curing reaction used G- capryl ester was bigger than that of used G-oligomer.

结果表明：固化体系在研究的固化温度下，转化率和反应速率在固化反应初期增加快速，温度越高增加越快，随着固化时间的延长，这种变化逐渐变慢，并最终趋于恒定；初期固化反应为一级反应，反应初期的表观活化能和指前因子大小顺序为G-预聚体＞G-辛酯。

We apply the results in Chapter 2 to the equitable list coloring of graphs and prove that a graph G is equitably △-choosable if G is a plane graph without i-cycles, 3 ≤ i ≤ 5, or a plane graph without 4-cycles(or 5-cycles) and 6-cycles.

从而得出不含3-圈，4-圈和5-圈的平面图和不含4-圈(或5-圈)和6-圈的平面图GΔ(G≥6均有一个Δ-均匀列表染色。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

Naturally, we can ask, in general, for the McKay quiver of G which is a finite subgroup of a generalized linear group, what its construction is and what relation there is with the conjugacy class of the group G.

我们自然会问：一般地，一般线性群的有限子群G的McKay箭图的构造如何以及它与群G的共轭类关系怎样？

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新