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METHODS:The 12～14 months old rats which sexual circle being disorder were chosed;the groups were Yanfuchun 5.0 g.kg-1,Yanfuchun 10 g.kg-1,Gengnianan 1.1 g.kg-1,model group and young controls;the rats were administered in continuousness orally Yanfuchun 5.0,10.0 g.kg-1,Gengnianan 1.1 g.kg-1 and the same volume of water for 22 days respectively.

选用12～14月龄动情周期紊乱的雌性大鼠进行实验，实验分延妇春5.0、10 g.kg-1；更年安1.1 g.kg－1，模型组及青年对照组；分别给大鼠连续灌胃延妇春5.0 g.kg－1。延妇春10 g.kg－1；更年安1.1 g.kg－1及等量蒸馏水22天。

In chapter 1 ,on one hand ,we give some sufficient conditions for a finite group to be supersolvable and nilipotent by using the properties of π-supplemented subgroups;For example: Theorem 3 Let G be a group, 2 ∈π, if every subgroup of G of prime order is contained in SE, every cyclic subgroup of G of order 4 is π-supplemented in G, then G will be supersolvable.

在第一章中，一方面我们利用π-可补子群的性质给出了有限群为超可解群及幂零群的若干充分条件；例如：定理3设G是群，2∈π，如果G的每个素数阶子群包含在SE中，G的每个4阶循环子群在G中π-可补，则G为超可解群。

Theorem 7 Let G be a group, if every subgroup of G of prime order is contained in Z_∞,2 ∈π, every cyclic subgroup of G of order 4 is π-supplemented in G,then G will be nilpotent.

定理7设G是群，G的素数阶子群包含在Z_∞中，2∈π，如果G的每个4阶循环子群在G中π-可补，则G为幂零群。

The calibration curve of the SM2-Kit with standard SM2 inhibitor was typical sigmoid curve fitted to the four parameter logistic equation with the linear detection of 0.625 μg/L to 80.0 μg/L (R2=0.9911), the sensitivity of 0.9 μg/L, the IC50 of 5.82 μg/L and the detection limit of 1 μg/L. The recoveries of SM2 spiked in pig feed were 83.4 %, in pig urine were 89.5 %. The precision and accuracy of the assay as determined by inter-assay and intra-assay coefficient variation was both below 15 %. The SM2-Kit generally had 2.08 % cross-reactivity towards sulfamerazine and little or no cross-reactivity towards other sulfonamides. The dilution solution of SM2 had no effect on results of SM2-Kit. The validity of SM2-Kit in 4 ℃ was above six months.

研究结果表明，SM2-Kit的标准曲线呈典型的S型，相关系数R2=0.9911，符合4参数logit曲线拟合，线性检测范围为0.625 μg/L～80 μg/L，灵敏度为0.9 μg/L，半数抑制浓度(IC50)为5.82 μg/L，检测限为1 μg/L；饲料样、猪尿样的平均添加回收率分别为83.4 %、89.5 %，平均批内和批间变异系数均低于15 %；SM2-Kit与磺胺甲基嘧啶的交叉反应率为2.08 %，与其它磺胺类药几乎没有反应性；基质对SM2-Kit的检测结果影响不大；试剂盒在4 ℃可保存6个月。

Rats living over 96h were considered survival. To observe the protective effects of ALR gene on acute liver injury rat, thirty-six rats that were injected peritoneally with 50% CCl4 of 2ml/kg were divided into the flowing 6 group according to doses (50?g/kg and 200?g/kg ) and administration routes of pcDNA3-ALR DNA 4h after CCl4 injection: group 1, model group; group 2, ALR gene of 50μg/kg was injected venally; group 3, ALR gene of 200?

另取36只大鼠按2ml/kg腹腔注射50% CCL4，染毒后4h按不同剂量（50μg/kg 和200μg/kg ）、不同注射途径（尾静脉注射、腹腔注射和联合注射）随机分为六组。1组：模型组，不注射ALR基因； 2组：尾静脉注射ALR基因 50μg/kg； 3组：尾静脉注射ALR基因 200μg/kg； 4组：腹腔注射ALR基因 50μg/kg； 5组：腹腔注射ALR基因 200μg/kg； 6组：联合注射（尾静脉和腹腔各注射ALR 基因100μg/kg）。

Result］ The color and elasticity of steamed breads made from 250 g flour added with 50 g cactus juice and 30 g amomum juice were bad and their surface was not smooth;when the addition amount of cactus juice was 70 g,the elastic extensibility of steamed breads was bad;when the addition amount of cactus juice was 90 g,the elasticity of steamed breads was good and their surfaces were smooth.

结果］ 在250 g面粉中加入50 g仙人掌汁、30 g砂仁汁蒸制的馒头色泽、弹性不佳，表面不光滑；加入70 g仙人掌汁时馒头的弹性延伸性不好；加入90 g仙人掌汁时，馒头的弹性佳，表面光滑。

Methods; Rat HSCs were treated with 0.4% fetal calf serum/DMEM for 48 hours and divided into the control group and experiment groups. HSCs in the control group were treated with 10% FCS, and HSCs in the experiment groups were treated with 10% FCS and low anticoagulative activity heparin with different concentrations (8 g/L, 40 g/L, 200 g/L, 1000 g/L, 5000 g/L). MTT reduction assay was used to evaluate the effect of low anticoagulative activity heparin on the growth of rat HSCs.

肝星状细胞经体积分数为0.4%胎牛血清／DMEM培养液同步化48h后分为对照组和实验组，对照组以体积分数为10%胎牛血清处理，实验组分别以体积分数为10%胎牛血清和不同质量浓度(8 g/L， 40 g/L， 200 g/L， 1000 g/L， 5000 g/L)低抗凝肝素处理，应用MTT法测定其对肝星状细胞生长的影响。

The whole peptidoglycan extracted by Sekine method and improved method have been done a comprehensive analysis of the structure and composition.Systemic structure and composition study of extracted whole peptidoglycan. We have determined that the bifidobacterium infantis whole peptidoglycan product contains 20.04 mg/g hexosamine, 500.01 mg/g total sugar, 36.84 mg/g total fatty acid, 339.6 mg/g protein, and 0.3 mg/g phosphate.

针对改良法和Sekine法所提取的完整肽聚糖做了全面的结构和成分分析，结果表明，采用改良法所提取的完整肽聚糖其超微结构完整，氨基己糖含量为20.04 mg/g；蛋白质含量为339.6 mg/g；中性糖含量为500.01 mg/g；总脂含量为36.84 mg/g；磷含量为0.3 mg/g。

Results When the culture medium was supplemented with 0 .05μg/ml sodium selenite,the proliferation of transformed cells was significant ly promoted and the transformation did not change remarkably,when supplemented w ith 0.25μg/ml,the proliferation was promoted but the transformation was inh ibited,and when with 0.75μg/ml and 1.50μg/ml,the proliferation was not pro moted and the transformation was strongly inhibited.Big dose of (1.50μg/ml) sodium selenite m ade the nuclei of the untransformed lymphocytes gather together like a few grape s doing,and even caused them to fuse.

结果 在培养物中添加0.05μg/ml亚硒酸钠能显著促进转化细胞增殖；添加0.25μg/ml可促进转化细胞增殖，但显著抑制细胞转化；添加0.75μg/ml和1.50μg/ml，不仅不能促进细胞增殖，而且强烈抑制细胞转化。1.50μg/ml剂量的亚硒酸钠使非转化淋巴细胞发生葡萄状聚集甚至核融合。

Methods: M-CSF-dependent bone marrow cells were isolated from 5-6 weeks old mice, and cultured in the presence of MCSF (25μg/L) with different concentrations of TNF-α(0, 1, 10, 100 μg/L) for 5 days, the formation of TRAP multinucleated cells was observed. These cells were also cultured in the presence of both RANKL (30 μg/L) and M-CSF (25 μg/L) with or without 10 μg/L TNF-α for4, 5, 6 and 9 days. The number of TRAP multinucleated cells and resorption pits on dentine slices were counted under light microscope.

选用小鼠巨噬细胞集落刺激因子依赖性非附着性骨髓细胞，在含有25μg/L M-CSF和0，1，10，100μg/L TNF-α的α-MEM培养液中培养5d后，观察抗酒石酸酸性磷酸酶染色阳性多核细胞的形成；细胞在含有25μg/L M-CSF和30μg/L sRANKL的α-MEM培养液中进行培养，比较加入和不加入10μg/L TNF-α培养4、5、6和9d后，所形成的TRAP多核细胞的数目和骨吸收面积。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。