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Sensitivity experiments showed that mycelial growth of the pathogen was affected by azoxystrobin,with a mean EC50as 0.964 9 μg/mL,ranging from 0.142 8 μg/mL to 1.950 9 μg/mL,and sporangial formation was affected by the fungicide to a greater extent,with a mean EC50as 0.023 9 μg/mL,ranging from 0.001 0 μg/mL to 0.084 4 μg/mL.

结果表明，嘧菌酯对辣椒疫霉的菌丝生长具有很强的抑制作用，EC50范围为0.1428～1.9509μg/mL，平均为0.9649μg/mL；对游动孢子囊形成的抑制作用更强，EC50范围为0.0010～0.0844μg/mL平均为0.0239μg/mL。

The average percent value of G C (49.3%) was less than that of A T (50.7%), there was a bias of the content of G, T in the third codon; The number of transition G-A and T-C were 22, 15 respectively, which were higher than that of transition A-G and C-T, the number of tranversion C-A, T-G is 2 respectively, and other tranversion patterns didnt occur; the probability of transition was higher than that of tranversion , Ts/Tv = 9.5-19, there was the highest number of transition in the third codon. The gamma parameter a of the lst,nd and 3rd codon positions were 0.00572,0.01237 and 1.05239 respectively, they showed that there was adifferent substitution rate at different codon position. Frequences of synonymous codon usage were relatively biased. The average rates of synonymous and nonsynonymous substitution were 0.0787, 0.0011 respectively, there was a significant difference between dS and dN (Z = 4.713, p.01), and the low ratio( CD = 0.0284 .3) of dN/dS ratio impled that there were selective constraints against the nonsynonymous sites in cyt-b gene, The distribution of nonsynonymous codon substitution pattern related to Grantharm distance indicated that the purified selection at 2nd codon positions was more intensive than that at 1st codon positions. The phylogenetic trees supported the view of the double origin of Chinese goose, which means that domestic Chinese goose was derived from Anser cygnoides and domestic European goose, and Yili was derived from Anser anser.

碱基含量分析可知，序列的G C含量(49.3％)＜A T含量(50.7％)，密码子第三位点的G、T含量都有较强的偏倚性；序列间G→A和T→C的转换数(22次和15次)高于A→G和C→T的转换数(10次和9次)，C→A、T→G颠换数均为2次，其余颠换模式均未发生；转换数明显高于颠换数，Ts／Tv=9.5～19，密码子第三位点的转换数最高，呈现了相当强烈的转换偏倚性；密码子第一、二和三位点的gamma分布参数α值分别为0.00572、0.01237和1.05239，表明密码子第一位点的替换速率变异最大，第二位点次之，第三位点的替换速率变异相对较小；编码同一氨基酸的同义密码子并非随机使用，表现出一定程度的使用偏倚性；同义替换速率和非同义替换速率分别为0.0787和0.0011，dS与dN值间的差异极显著(Z=4.713，p＜0.01)，而ω=0.0284，明显小于0.3，表明雁属鹅细胞色素b基因序列经历了中度净化选择作用；单步非同义替换(Sing-step nonsymonymous codon substitution，SSNCS)分布模式与Grantharm距离之间的关系说明密码子的三个位点所受的净化选择强度不同；构建的最大简约树与邻接树拓扑结构一致，支持中国家鹅的双起源学说，即除伊犁鹅外的其它中国鹅品种起源于鸿雁，伊犁鹅和欧洲的郎德鹅、莱茵鹅起源于灰雁。

The different concentration (160μg/ml, 80μg/ml, 40μg/ml, 20μg/ml, l0μg/ml) of NAPP water solution was respectively added into cell culture fluid of 2. 2. 15 cell strain and the inhibitory effect of APP on HBeAg was evaluated.

将NAPP水溶液160μg/ml，80μg/ml，40μg/ml，20μg/ml，10μg/ml分别加入HePG2.2.15细胞株培养悬液中，8d后检测上清液中HBeAg的含量，并计算HBeAg抑制率、细胞存活率、HBeAg抑制率为50%时的药物浓度、实验组存活细胞为对照组的50%时的药物浓度和治疗指数。

Mean daily consumption of alcohol per day was greater in men than in women (28.2 25.0 g vs 13.9 15.0 g), as reflected in both in the lowest (mean, 4.1 g vs 1.1 g) and highest (mean, 68.7 g vs 38.8 g) quintiles of daily consumption. More than 50% of women consumed less than 10 g a day of alcohol, and more women were abstainers than men (3.0% vs 2.1%).

男性的平均酒精日摄取量要比女性高28.2 ( 25.0 g vs 13.9 ( 15.0 g)；最低(平均， 4.1g vs 1.1g)及最高(平均， 68.7g vs 38.8g)的日摄取量也是男性高於女性，超过10%的女性，日摄取量低於10 g；对酒精节制的女性人数也高於男性(3.0% vs 2.1%)。

Mean daily consumption of alcohol per day was greater in men than in women (28.2 25.0 g vs 13.9 15.0 g), as reflected in both in the lowest (mean, 4.1 g vs 1.1 g) and highest (mean, 68.7 g vs 38.8 g) quintiles of daily consumption. More than 50% of women consumed less than 10 g a day of alcohol, and more women were abstainers than men (3.0% vs 2.1%).

男性的平均酒精日摄取量要比女性高28.2 ( 25.0 g vs 13.9 ( 15.0 g)；最低(平均， 4.1g vs 1.1g)及最高(平均， 68.7g vs 38.8g)的日摄取量也是男性高于女性，超过10%的女性，日摄取量低于10 g；对酒精节制的女性人数也高于男性(3.0% vs 2.1%)。

So we selected thevinblastine optimum parameters were 0.1μg/ml and 8 hour cultured duration.The fourth, there were significantly differences percent between 10 hourgroups and other three groups in 0.1μg/ml groups; and there were no significantlydifferences between 8 hour groups and 10 hour groups, but they had significantlydifferences among other two groups in 0.3μg/ml groups; and there weresignificantly differences between 10 hour groups and other three hour groups in0.5μg/ml groups; and there were significantly differences between 10 hour groupsand other three groups in 0.7μg/ml groups on the metaphases (P＜0.05), in theexperiment of the effect on the mouse 8- cell embryo stage single blastomere ofvinblastine with different concentrations and duration, by x~2 statistical analysis.

根据实验的实际情况，适宜制备小鼠4-细胞期胚胎单卵裂球染色体标本的长春花碱的处理浓度和时间是0.1μg／ml和8小时。4、在确定长春花碱不同处理浓度和时间对小鼠8-细胞期胚胎单卵裂球中期分裂相数影响的实验中，经统计分析，阻断培养0.1μg／ml浓度组中10小时组与其他三组差异显著；阻断培养0.3μg／ml浓度组中8小时组和10小时组间差异不显著，但与其他两组差异显著；阻断培养0.5μg／ml浓度组中10小时组与其他三组差异显著；阻断培养0.7μg／ml浓度组中10小时组与其他三组差异显著(P＜0.05)。

So we selected the colchicine optimumparameters were 0.2μg/ml and 6 hour cultured duration.The third, there were significantly differences percent between 10 hourgroups and other three groups (4 hour groups, 6 hour groups and 8 hour groups) in0.1μg/ml groups; and there were significantly differences between 8 hour groupsand 10 hour groups, but they had no significantly differences among other twogroups in 0.3μg/ml groups; and there were significantly differences between 10hour groups and 6 hour groups, but 10 hour groups had no significantlydifferences among other two groups in 0.5μg/ml groups; and there weresignificantly differences between 8 hour groups and other three groups in0.7μg/ml groups on the metaphases (P＜0.05), in the experiment of the effect onthe mouse 4- cell embryo stage single blastomere of vinblastine with differentconcentrations and duration, by x~2 statistical analysis.

根据实验的实际情况，适宜制备小鼠8-细胞期胚胎单卵裂球染色体标本的秋水仙素的处理浓度和时间是0.2μg／ml和6小时。3、在确定长春花碱不同处理浓度和时间对小鼠4-细胞期胚胎单卵裂球中期分裂相数影响的实验中，经统计分析，阻断培养0.1μg／ml浓度组中10小时组与其他三组差异显著(4小时组、6小时组和8小时组)；阻断培养0.3μg／ml浓度组中8小时组和10小时组间差异不显著，但与其他两组差异显著；阻断培养0.5μg／ml浓度组中10小时组与6小时组差异不显著，而与其他两组差异显著；阻断培养0.7μg／ml浓度组中8小时组与其他三组差异显著(P＜0.05)。

The excitation and emission wavelengths in the measurement are 345 nm, 430nm respectively. When the concentrations of FWAs are from 0 to 1.0 μg/mL, they have a linear relationship with the emitted fluorescence intensity. In this interval, the calibration curve is y=0.001 7x-0.018 7, and R2 is 0.999 2, the limits of detection and quantification are 4 μg/g and 10 μg/g respectively, and the average percentage of recovery is 95.3%, which meet the measuring requirements.

该方法激发波长为345 nm，发射波长为430 nm；定量标准曲线为y=0.001 7x-0.018 7，在0～1.0 μg/mL范围内线性相关性R2=0.999 2；方法检出限4 μg/g，定量限10μg/g，平均加标回收率为95.3%，可满足检测要求。

Heating the mixture of low-valued fish hydrolysates 100 mL,meat hydrolysates 10 mL,glucose 1.8 g,xylose 0.4 g,cysteine 0.75 g,vitamin B_(1) 0.75 g and ginger juice 2 g at pH 6.5,120 ℃ for 40 min is optimal for sauce flavor synthesis in which meat smell is strengthened ...

用小杂鱼酶解液100 mL；肉酶解液10 mL；葡萄糖1.8 g；木糖0.4 g；半胱氨酸盐酸盐0.75 g；维生素B10.75 g；生姜汁2 g；调节pH值至6.5~7.0，120℃加热40 min能够形成逼真肉香，无鱼腥味的调味料。

HL60 cells with same density was divided into 5 groups at random , added with 0 μ g/ml, 5 μg/ml, 25 μg/ml, 50 μg/ml and 75 μ g/ml ChS.

同一密度的HL60细胞随机分为五组，加入0μ g/ml、5μ g/ml、25 μ g/ml、50 μ g/ml和75 μ g/ml ChS。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。