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The female salivary gland proteins were influenced by JH Ⅲ. Treatment on d2 after feeding, 10μg dose increased SG protein level and restrained 136kD protein gene expression. Treatment on d0 after engorgement, 10μg, 50μg and 100μg doses significantly increased the d2 SG protein and 100μg dose made 35kD protein gene expression, 200μg treatment made the 136kD and 192kD proteins absent. This is the first found that JHs regulate arthropoda SG activity.

保幼激素对雌蜱唾液腺蛋白及成分有影响，吸血后2天处理，10 μg剂量使唾液腺蛋白含量明显增加，抑制136kD蛋白的基因表达；饱血当天处理，10μg、50μg和100μg显著提高饱血后2天唾液腺蛋白含量，100μg使35kD蛋白表达，200μg使136kD和192kD的蛋白缺矢；饱血当天处理，1μg和10μg剂量均能显著提高饱血后4天雌蜱唾液腺蛋白含量，高剂量（100μg）抑制136kD蛋白的表达，该结果首次报道了保幼激素对节肢动物唾液腺的调控作用。

Aldrin at concentrations higher than 40 μg/L decreased life expectancy at birth of F1 generation of the cladoceran, that at 20 μg/L and 80-640 μg/L decreased net reproduction rate, that at 320 and 640 μg/L shortened generation time, and that at all the concentrations decreased intrinsic rate of population increase. Aldrin at 160-640 μg/L shortened life expectancy at birth and generation time of F2 generation of the cladoceran, that at 160 and 640 μg/L decreased net reproduction rate, but that at all the concentrations except for 40 and 320 μg/L increased intrinsic rate of population increase.

浓度高于40μg/L的艾氏剂使多刺裸腹溞F1代的生命期望显著缩短，20μg/L和80~640μg/L的艾试剂使F1代的净生殖率显著降低，320μg/L和640μg/L的艾氏剂使F1代的世代时间显著缩短，所有浓度的艾试剂均使F1代的种群内禀增长率显著降低。160~640μg/L的艾氏剂使多刺裸腹溞F2代的生命期望和世代时间显著缩短，160μg/L和640μg/L的艾氏剂使F2代的净生殖率显著降低；除40和320μg/L外，所有浓度的艾试剂均显著提高了F2代的种群内禀增长率。

The amount of hydroxyproine in the cell wall from eight kinds of vegetables were determined in the present paper. The result showed that the amount of hydroxyproine in the cell wall from skin of soja, legume, pollen, carrot root, cole, celery, green grocery and onion was 6.02μg/mg, 3.30μg/mg, 1.74μg/mg, 1.53μg/mg, 0.64μg/mg, 0.52μg/mg, 0.42μg/mg and 0.31μg/mg, respectively.

首先测定8种蔬菜细胞壁中的羟脯氨酸含量，研究结果表明：豆皮、豆荚、花粉、胡萝卜根、油菜叶柄、芹菜叶柄、青菜和洋葱细胞壁中羟脯氨酸的含量分别为6.02μg/mg、3.30μg/mg、1.74μg/mg、1.53μg/mg、0.64μg/mg、0.52μg/mg、0.42μg/mg和0.31μg/mg。

The patients were at random divided as yizhi capsule group (47 cases) and control group (47 cases)METHODS: Medicine for patients in the yizhi capsule group: Yizhi capsule, made from processed flowery knotweed 14 g, ginseng 4 g, acorus 8 g,polygala 6 g, leech 10 g, epimedium 10 g, anemarrhena 6 g, ligasticum 12g, with each capsule contenting 0.228 g, which was consistent with 10 g raw drug, orally, 6 capsules, 3 time a day.

随机分为益智胶囊组47例和对照组47例。方法：益智胶囊组用药：益智胶囊成分为制何首乌14 g，人参4 g，石菖蒲8 g，远志6 g，水蛭10 g，淫羊藿10 g，知母6 g，川芎12 g，每粒胶囊含内容物0.228 g，相当于生药10 g.6粒/次，3次/d，口服。

Calidonian granites are widely developed in the Yema Nanshan area in the western segment of the Qilian orogenic belt. The geochemical characteristics of most of the granites are similar to those of adakites: SiO2≥56%, Al2O3≥15.0%, low MgO (average 2.04%), high Sr (257-889μg/g, average 546.42μg/g), low Y (7.1-25.3μg/g,average 16.67μg/g), average Sr/Y=5.89, no pronounced Eu anomaly and Sr positive anomaly. Adakites in this area have five rock types, quartz diorite, quartz monzodiorite, quartz monzonite, monzogrannite and hornblende granite. These rocks commonly contain hornblende, belonging to I-type granites.

提要：祁连西段野马南山一带发育大量的加里东期花岗岩，其中大部分花岗岩具有与adakite岩相似的地球化学特征，SiO2≥56%， Al2O3≥15.0％，低MgO（平均2.04％），高Sr(257～889μg/g，平均值546.42μg/g)，低Y（7.1～25.3μg/g，平均16.67μg/g），Sr/Y平均为35.89μg/g，Eu无明显负异常，Sr正异常，Adakite岩石类型有石英闪长岩、石英二长闪长岩、石英二长岩、角闪花岗岩、二长花岗岩，岩石中普遍含有角闪石，属I型花岗岩，地球化学图解反映该花岗岩形成环境为板块碰撞前的岛弧型，锆石U-Pb同位素年龄为444+38/-33 Ma，处在祁连造山带板块俯冲收缩阶段，因此可以推断野马南山埃达克质Adakite花岗岩是俯冲板片部分融熔和壳幔岩浆混合形成的。

To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry，FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时，对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后，Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR，RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时，Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope，LSM)观察北豆根总碱(0、40μg/ml)作用24小时后，Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

Fluorescent instruments.the colonies of the specimens yielding growth were identified by the vitek32 instruments and kb method was used for the drug sensitivity tests.results three hundred and thirtyone bacteria strains were isolated from a total of 3104 blood culture specimens,with a positive rate of 10.7%.of the identified bacteria,g+ cocci accounted for 50.3%,g-bacilli accounted for 44.7%,fungi accounted for 3.0%,and anaerobian accounted for 1.1%.the susceptibility rates of g-bacilli to imipenem,amikacin and cefoperazone/sulbactam were high.the susceptibility rate of g+ cocci to vancomycin and imipenem were also high.conclusion g+ cocci prevail over g-bacilli in the blood specimens.the staphylococci are the chief bacteria in the childrens blood specimens.both g+ cocci and g-bacilli are sensitive to imipenem.

结果 在3 104份血液培养标本中分离出病原菌331株，阳性检出率为10.7%。病原菌以革兰阳性需氧球菌居首位（50.3%），革兰阴性需氧杆菌次之（44.7%），真菌占3.0%，厌氧菌占1.1%。血液培养中的g+球菌对万古霉素和亚胺培南较为敏感，g-杆菌对亚胺培南、舒普深、丁胺卡那较为敏感。结论血液培养病原菌以g+球菌为主，g-杆菌次之；儿童血液培养病原菌以葡萄球菌属为主；亚胺培南对g+球菌和g-杆菌均具有较高的敏感率。

Compared with control,①the mean tumor weight of H22 of SXKA Granules three dose groups were decreased significantly(P.01,P.05),and the mean inhibition rates of SXKA Granules 20、10 g/kg dose groups were above 30%;②the mean tumor weight of S180 of SXKA Granules three dose groups were decreased significantly(P.01),and the mean inhibition rates of SXKA Granules 20、10 g/kg dose groups were above 32%;③the mean tumor weight of EAC of SXKA Granules three dose groups were decreased significantly ( P.01, P.05),and the mean inhibition rates of SXKA Granules 20 g/kg dose groups were above 38%;④the mean tumor weight of Lewis carcinoma of SXKA Granules three dose groups were decreased significantly(P.01, P.05),and the mean inhibition rates of SXKA Granules 20、10、5 g/kg dose groups were above 36%;⑤the mean tumor weight of W256 of SXKA Granules three dose groups were decreased significantly ( P.01, P.05),and the mean inhibition rates of SXKA Granules 20、10 g/kg dose groups were above 32%;⑵Compared with control,SXKA Granules 20、10 g/kg dose groups had extended the survial time of the P388-bearing mice respectively(P.01),and the mean prolong rates of SXKA Granules 20、10 g/kg dose groups were above 50%;⑶Compared with S180-bearing group, SXKA Granules 20、10 g/kg dose groups could increase the weight of thymus and spleen, Spleen index and thymus index were increased, SXKA Granules 5 g/kg dose group could increase thymus index(P.05);⑷As Compared with control group, SXKA Granules 20、10 g/kg dose groups could improve mouse serum half hemolysis value depressed by transplanted tumor dramatically(P.01), which revealed the SXKA granules could improve the mouse humoral immunity system;⑸SXKA Granules 20 g/kg dose group could increase of englobe indexαon S180-bearing mice remarkably(P.01), which indicated the SXKA Granules could improve their cellular immunity system.

对荷W256大鼠，生兴克癌冲剂20、10、5 g / kg三组的平均瘤重明显低于对照组(P.01，P.05)，生兴克癌冲剂20、10 g / kg组的平均肿瘤抑制率均大于32 %；⑵与空白对照组相比，生兴克癌冲剂20、10 g/ kg能显著地延长移植小鼠白血病P388小鼠的存活天数(P.01)，生兴克癌冲剂20、10 g/ kg对荷白血病P388小鼠生命延长率均在50%以上；⑶与S180荷瘤组相比，生兴克癌冲剂对荷瘤鼠的免疫器官重量、胸腺指数和脾指数有一定的提高趋势，其中生兴克癌冲剂5 g / kg组对荷瘤小鼠的胸腺指数有一定的提高作用(P.05)；⑷与S180荷瘤组相比，生兴克癌冲剂20、10 g /kg组可提高由荷瘤引起的小鼠血清半数溶血素值的降低(P.01)，表明其可提高荷瘤小鼠体液免疫功能；⑸与对照组相比，生兴克癌冲剂20 g /kg组可提高荷S180肉瘤小鼠的免疫吞噬系数α值(P.01)，表明其可提高荷瘤小鼠细胞免疫功能。

The results showed that the optimal auto-induction medium is: tryptone 19.17 g/L, yeast extract 9.59 g/L, Na2HPO4 5.72 g/L, KH2PO4 5.48 g/L,(NH4)2SO4 2.66 g/L, NaCl 3.33 g/L, glycerol 2%, glucose 0.68 g/L, lactose 6.33 g/L, MgSO4

实验结果表明，最优培养基成分为蛋白胨19.17 g/L，酵母膏9.59 g/L， Na2HPO4 5.72 g/L， KH2PO4 5.48 g/L，(NH4)2SO4 2.66 g/L， NaCl 3.33 g/L，甘油2%，葡萄糖0.68 g/L，乳糖6.33 g/L， MgSO4

The extract of Agrocybe Cylindracea was rich in metal elements, including calcium(3.607mg/g), magnesium(3.021mg/g), zincum(79.3μg/g), iron(79.2μg/g).

茶树菇提取物含有丰富的钙(3.607 mg/g)、镁(3.021 mg/g)、锌(79.3μg/g)、铁(79.2μg/g)等元素，对机体有害的重金属元素铅(0.91μg/g)、砷(0.41μg/g)含量相对较低。

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。