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METHODS: Fetal liver was obtained sterilely. Monoplast suspension was collected by collagenase digestion and mechanical separation, and then centrifuged using 1.070 g/mL Percoll separating medium and 1.077 g/mL Ficoll separating medium. Cells achieved from the interface of separatory liquids were cultured in DMEM/F12 medium, supplemented with 0.1 volume fraction of fetal bovine serum, 20 μg/L hepatocyte growth factor and 40 μg/L epidermal growth factor, for 9 days.

无菌状态下取出胎儿肝脏，以胶原酶消化法和机械分离法获得胎儿肝脏来源单细胞悬液，分别采用1.070 g/mL Percoll分离液和1.077 g/mL Ficoll分离液进行密度梯度离心，吸取界层细胞，添加含体积分数为0.1 FBS、20 μg/L肝细胞生长因子、40 μg/L表皮生长因子的DMEM/F12新鲜培养基诱导9 d。

With retention time prolonged, the concentrations of TN, NH4(superscript +)-N and TP of domestic sewage were obviously reduced in all floating-bed systems, however the NO4(superscript +)-N concentrations were gradualjy accumulated due to the stronger nitrification than denitrification. The plants in floating-bed system increased significantly the N2O flux, which was 174.44μg/(m^2h), 82.19μg/(m^2h), 112. 49μg/(m^2h) and 44.81μg/(m^2h), respectively, for the Lolium mutliflorum, Oenanthe javanica, Vetiveria Zizanioides arid none-plant floating-bed systems.

净化周期内，浮床各系统的TN、NH4-N和TP浓度随着污水停留时间的延长直线下降，而NO3-N浓度却因系统内硝化强度大于反硝化强度而产生了累积；植物的存在明显的促进了浮床系统的N2O排放，浮床黑麦草、水芹、香根草和对照系统N2O的平均排放通量分别为174.44μg/， 82.19μg/， 112.49μg/和44.81μg。

The adsorption results of triethylene tetraamine-modified oxycellulose showed that:(1) when adsorption tests were carried at 37℃and pH7 in 5 h,static adsorption capacity of 0.5g adsorbent for urea(6g/L in dialysis solution),uric acid(1 g/L in redistilled water) and creatinine(0.1 g/L in dialysis solution) were 131.5mg/g,24.6mg/g,3.31mg/g respectively.

三乙烯基四胺基氧化纤维素的吸附性能结果表明：(1)吸附温度为37℃，pH值为7时，0.5g吸附剂对于尿素(6g/L透析液)、尿酸(1g/L二次水)、肌酐(0.1g/L透析液)在5h的静态吸附容量分别为131.5mg/g、24.6mg/g、3.31mg/g。

The changes of muscle protein content and pH values were observed. Results Thymus and spleen coefficients in high and low dosage group were 9.1±2.3, 6.3±1.1, respectively, and those of in the controll were 6.5±1.6, 3.4±0.5.The contents of muscle protein (g/100 g) in high, medium, and low dose groups were 24.68±0.55, 22.48±0.54, 21.22±1.25, respectively, lower than that of the control (31.83±2.36, P.05). Paramorphia of lymphocyte in high dose ciprofloxacin treated group was observed.

结果 对照组胸腺和脾脏系数分别为(9.1±2.3)，(6.3±1.1)mg/g，高剂量组胸腺和脾脏系数分别为(6.5±1.6)，(3.4±0.5)mg/g，与对照组比较，差异均有统计学意义（P.05或P.01）；而中、低剂量组的脾脏指数与对照组比较，差异均有统计学意义(P.05)；环丙沙星各剂量组与对照组比较，肌肉pH值变化不明显；环丙沙星高、中、低剂量肌肉蛋白质含量分别为(24.68±0.55)，(22.48±0.54)，(21.22±1.25)g/100 g，与对照组(31.83±2.36)g/100 g比较明显下降(P.05)；与对照组比较，高剂量组脾淋巴细胞形态异常。

The results indicate that the presence of GSH increased the expression of exoY、exoS、phzA1 and gacA,while no difference was observed with others virulence factor genes tested.PAO1 produced markedly less pyocyanin than the wild type strain PAOIP＜0.01Treating with GSH, the PCN production of PAOI(△gshB was increased from 0.47μg/mL to 1.73μg/mL (P＜0.01),the PCN production of PAO1 increased from 1.3μg/mL to 1.9μg/mL(P＜0.05),this result indicates that GSH upregulates PCN production.

结果发现，GSH对启动子库中exoS、exoY、phzA1和gacA等致病性因子的表达有促进作用，对其余测试过的致病性因子则没有明显影响；铜绿假单胞菌分泌的重要致病因子绿脓菌素(pyocyanin，PCN)的含量，在PAO1中远低于PAO1P＜0.01加入GSH后，PCN平均含量在PAO1(△gshB中从0.47μg/mL提高到1.73μg/mL，差异十分显著(P＜0.01)，在PAO1中从1.3μg/mL提高到1.9μg/mL，差异显著(P＜0.05)，表明GSH对铜绿假单胞菌生产PCN有促进作用。

In the special case when G = AutX, we call a G—vertex-transtive graph, G—edge-transtive graph, G—semisymmetric graph X is vertex-transtive edge-transtive semisymmetric graph respectively In this paper we investigate the autmorphism graph AutX of the cubic semisymmetric graph of order 12p ,when p is primes.

特别地，若G=AutX这时的G-点传递图、G-边传递图、G-半对称图称为点传递图边传递图半对称图。

Result The main components of aqueous infusion of pepper leaf were vanillic acid (75.7 μg/g) and umbelliferon (29.8 μg/g).

结果］辣椒叶水浸液的主要成分为香草酸(75.7μg/g)和伞花内脂(29.8μg/g)。

The results showed that in the growth period of R. glutinosa, soil ferulic acid, vanillic acid, and vanillin contents had a persistent increase, while β-hydroxybenzoic acid content increased first but decreased then. The addition of ferulic acid (8 μg·ml-1), vanillic acid (08 μg·ml-1), vanillin (12 μg·ml-1), and β-hydroxybenzoic acid (30 μg·ml-1) in water culture had stronger inhibitory effects on R. glutinosa root length, root mass, fresh plant mass, and plant height. Treating with test phenolic acids except β-hydroxybenzoic acid increased leaf chlorophyll content significantly, compared with the control. After treated with test phenolic acids, plant SOD and POD activities decreased after an initial increase, and plant malondialdehyde content increased. In the treatment of ferulic acid, root SOD and POD activities were the lowest, and roots were rotted and dead.

结果表明：阿魏酸、香草酸和香草醛含量在地黄生长期表现为持续增加，而对羟基苯甲酸含量先升高后降低；外加阿魏酸（8 μg·ml-1）、香草酸（0.8 μg·ml-1）、香草醛（1.2 μg·ml-1）和对羟基苯甲酸（3.0 μg·ml-1）对水培地黄根长、根质量、全株鲜质量和株高有较强的抑制作用；酚酸处理使地黄叶片叶绿素含量降低，除对羟基苯甲酸外差异性极显著；经过酚酸处理的地黄幼苗SOD和POD活性为先升高后下降，丙二醛含量升高，其中阿魏酸的处理使根中酶活性降至最低，根部腐烂死亡。

Under illumination condition,the ED50 and ED90 of direct virucidal activity of C60 to HSV-1F strain are 0.05313 μg/ml and 0.1696 μg/ml respectively,and the ED50 and ED90 of infection block action of C60 are 0.9461 μg/ml and 5.643 μg/ml separately.

在光照条件下，对HSV-1F株直接杀灭的ED50、ED90分别为0.05313 μg/ml、0.1696 μg/ml；感染阻断的ED50、ED90依次为0.9461 μg/ml、5.643 μg/ml。

The ED50s of direct virucidal activity,the inhibition effects on the multiplication and infection of CVB3 of ribavirin are 6.26μg/ml,7.55μg/ml,7.50μg/ml respectively,the corresponding figures of emodin are 1.54μg/ml,8.34μg/ml,3.06μg/ml separately.

病毒唑对CVB3直接杀灭、增殖抑制、感染阻断的ED50分别为6.26、7.55、7.50μg/ml，大黄素的相应数值分别为1.54、8.34、3.06μg/ml。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。