查询词典 G-clamp

METHODS: 15 g/L silk fibroin solution was made by degumming, salvation and dialysis. Chitosan was dissolved in 2% acetic acid solution to prepare 25 g/L chitosan-acetic acid solution. Two solutions were mixed to prepare six silk fibroin/chitosan solutions, and mass ratio was 10: 0, 5: 5, 4: 6, 3: 7, 2: 8, 0: 10. These solutions were separately sucked in a 24-well plate. Following exhausting gas vacuole at 4 ℃, precooling was performed at -20 ℃ for 12 hours, followed by cryodesiccate for 30 hours.

通过脱胶、溶解、透析这3个主要步骤制成质量浓度为15 g/L的丝素溶液，将壳聚糖溶解在2%的醋酸溶液中制成25 g/L的壳聚糖-乙酸溶液，然后将两者混合，配制成丝素/壳聚糖质量比分别为10∶0，5∶5，4∶6，3∶7，2∶8，0∶10的6种溶液，分别吸入24孔板中，4 ℃排出气泡后，-20 ℃预冷冻12 h，再冷冻干燥30 h。

Results The linear ranges of tanshinol sodium and protocatechualdehyde were 42.52~382.68μg/mL and 8.14~ 73.30μg/mL, respectively; the correlation coefficients were 0.999 8 and 0.999 6, respectively; the average recoveries of spiked sample were 100.54%(RSD=0.797%) and 98.87%(RSD=1.282%), respectively.

结果丹参素钠在42.52~382.68μg/mL浓度范围内线性关系良好（r＝0.999 8），平均回收率为100.54%（RSD＝0.797%）；原儿茶醛在8.14~73.30μg/mL浓度范围内线性关系良好（r＝0.999 6）；平均回收率为98.87%（RSD＝1.282%）；结论此法简便，快速，准确。

The results indicated that 67-V medium supplied 30g/L sucrose, 200 g/L potato extract, 100 mg/L 〓 and 800 mg/L 〓 wasbest for the growth of protocorm when 100 ml liquid medium was put in 250 ml flaskand inoculum was more than 6 g/flask.

结果表明，有利于原球茎生长的最佳培养基组合为：67-V基本培养基+30g／L蔗糖+200 g／L马铃薯提取汁+100 mg／L〓+800 mg／L〓，采用250 ml三角瓶加入100 ml培养液，接种量大于6 g／flask，可获得较大的生物量。

During the ESR study of the effects of oxLDL on the ESR signal intensity of hydroxyl radicals in collagen (2 μg/ml)-activated platelets, we found that incubation of human platelets with collagen produced a typical four-line hydroxyl radical signal and a long-lived g = 2.005 radical detectable by spin trapper 5,5-dimethyl-1 pyrroline N-oxide.

在研究oxLDL降低由collagen 〈2 μg/ml〉刺激血小板产生的氢氧自由基〈OH●〉的ESR讯号强度的同时，我们发现以DMPO当自由基捕捉剂，collagen除了刺激血小板产生一个四线的典型氢氧自由基之外，还产生一个长半衰期 g = 2.005的自由基。

The yield of pyruvaldehyde in glucose/glycine system (181□g/100ml)was3.6 folds that of galactose/glycine system (50□g/100ml)at 48 hr,We also founded that higher tmeperature promoted the change of pH, which in turn effect more significantly on Maillard reaction.

在丙酮醛产量方面，45°C组葡萄系统在6084小时后才开始产生且量较少；而60°C组葡萄糖系统其产量於48小时达至最高（181□g/100ml），此时丙酮醛的产量为乳糖组（50□g/100ml ）的3.6倍，之后有下降的趋势，其中浓度的效应较不明显。

Electrochemical measurements of the singler A2B7 and A5B19 phase alloy showed that, the activation properties of singler type A2B7 and AsB19 phase composition alloys were the same. The maximum discharge capacity (386.12mAh/g) of A2B7 type phase alloy was slightly higher than the maximum discharge capacity (371.38mAh/g) of A5B19 type phase alloy. The cyclic stability of alloy electrode of both A2B7 and A5B19-type phase alloy were all very well. The capacity retention rate of 100 cyclic was more than 85%.The high-rate discharge (HRD900=85%) of AsB19phase alloy was higher than the high-rate discharge (HRD900=76.6%) of A2B7phase alloy.

对较单一的A2B7与A5B19型物相合金进行电化学测试，结果表明：A2B7型相结构储氢合金的活化次数与A5B19型相结构储氢合金的活化次数基本相同，A2B7型物相合金的最大放电容量(386.12mAh/g)略高于A5B19型物相合金的最大放电容量(371.38mAh/g)。A2B7与A5B19型物相合金电极100个循环容量保持率都在85%以上，且差别不大。A5B19型相结构合金的高倍率放电性能(HRD900=85%)高于A2B7型相结构合金的高倍率放电性能(HRD900=76.6%)。

Results ①Five days after abdominal application of Xiaoji power(0.1 g per mouce),the medicine showed a very significant effect in enhancing gastrointestinal propulsive function.② Xiaoji powder not only had relaxing effect on the normal smooth muscle in vitro,but also had a strong spasmolytic effect on acetylcholine,green bariuminduced spasm of intestinal smooth muscle.The experiment was based on 10 μg atropine sulfate as a positive control to carry out control experiments.It showed the graphics of atropine sulfate in spasmolytic role.③Xiaoji powder navel paste therapy played a positive role in adjusting the gastrointestinal electric disorder caused by deficiency of spleen Qi,and could restore it to its normal state.

结果 （1）以0.1 g/只消积散给予小鼠腹部用药5 d，即显示非常显著的增强胃肠推进功能作用；（2）消积散不仅对正常离体平滑肌有舒张作用，同时对由于乙酰胆碱、氯化钡引起的肠平滑肌痉挛也有强的解痉作用，该实验是以硫酸阿托品10 μg作为阳性对照药进行对照实验，显示了硫酸阿托品的解痉作用图形；（3）消积散敷脐对脾虚引起的胃肠电紊乱有明显的调节作用，使脾虚大鼠的胃肠电基本恢复到正常状态。

Get the neonate Sprague-Dawley rats medulla spinalis, through the conventional primary cell culture procedure, we use the method of difference adherence time and get the motor neurons of anterior spinal cord. When the neuron is in maturity, we make the mechanical injury model in vitro. All the models were divided into four groups: group A is control group; group B is 100μM ATP group; group C is 100μM ATP+20μg/ml suramin group and group D is 100μM ATP+10μM ouabain+10μg/ml Thapsigargin group. Culture the four groups neurons, after one day, we count the motor neuron, observe the survival and activity of neurons through MTT shade selection experiment, use flow cytometry to analyze the percentage of apoptosis of motor neurons of anterior spinal cord and detect the expression of protein p-GSK-3β(ser9) through Western-Blot technology.

方法取新生大鼠脊髓，通过常规的原代细胞培养程序，采用差速贴壁法分离出大鼠脊髓前角运动神经元，培养成熟后制作神经元机械损伤体外模型，分为A组：对照组、B组：100μMATP组、C组：100gMATP+20μg/ml Suramin组和D组：100μM ATP+IOμM Ouabain+10μg/ml Thapsigargin组，对各组机械损伤的运动神经元进行培养，1天后分别进行运动神经元计数、MTT比色实验观察运动神经元的存活及活性、流式细胞仪分析机械损伤的脊髓前角运动神经元凋亡百分率和Werstern Blot技术检测p-GSK-3β(ser9)的表达。

Results: good standard linear curve, r = 0.9998; minimum checking limited to: 0.28μg formaldehyde, or 1.4μg sodium formaldehyde sulfoxylate; Variation coefficient 2.0%.

结果：标准曲线线性良好，r = 0.9998；最低检出限为：0.28μg甲醛，或1.4μg吊白块；变异系数为2.0 %。

The syntype MOV surge protection can withstand the current as high as 6000A and offers shield for three lines: L-N, L-G, and N-G. Besides, it has the function of failure indicator and protection. When the protection module failed, it will disconnect and indicate the user to change one.

全模式 MOV 电涌防护，防护能力最高达到6000A ， L—N 、 L—G 、 N—G 三线保护，具失效保护及指示功能，当保护模块失效时，能及时退出并及时提示使用者更换保护模块。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。