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The cardiomyocyte viability were detected by MTT assay for calculating the survival rate and the inhibitory rate of the virus. Myocardial F-actin and VEGF were analyzed by immunofluorescent staining with confocal microscopy. Mean fluorescent intensity of F-actin and VEGF were determined by flow cytometry.Results There is no toxicity on cardiomyocytes when PD is at concentration of 0.02 mmol/L and 0.2 mmol/L while there is toxicity at concentration of 2 mmol/L. CVB3 could reduce the viability of cardiomyocytes, depolymerize F-actin cytoskeleton and reorganize VEGF protein.

体外培养心肌细胞,用100 TCID50柯萨奇B3病毒(CVB3)感染心肌细胞,建立病毒性心肌炎实验模型,然后分别用0.02mmol/L、0.2mmol/L、2mmol/L三种不同浓度的PD处理感染CVB3病毒的心肌细胞,观察心肌细胞的形态和搏动,用细胞免疫荧光技术标记纤维状肌动蛋白和VEGF蛋白,四唑蓝比色法测定心肌细胞活性和病毒抑制率,流式细胞仪检测各组心肌细胞VEGF蛋白和F-actin平均荧光强度。

Based on an analysis of distribution pattern of hydrocyclone"s axial velocity, by changing the inlet flow rate Qi and split ratio Fand making an investigation in the effect of Qi and F on axial velocity field, the factors causing the forming of short-cut circuit and circulating flow are found. It is considered that the short-cut circuit and circulating flow are produced by the structure of the hydrocyclone, and can"t be eliminated completely, though they interfere the flow field inside a hydrocyclone and affect the separation accuracy. Test results show that, by increasing Qi and F and controlling them in a certain range, the separation efficiency of the hydrocyclone can be improved.

在研究旋流器轴向速度分布规律的基础上,分析了短路流与循环流的形成原因,试验研究了改变操作参数,即改变入口流量Qi与分流比F对轴向速度场的影响认为短路流与循环流是由旋流器结构决定的,很难完全消除,它们干扰了旋流腔和大锥段的流场,在一定程度上也影响了分离精度试验结果表明,加大入口流量Qi与分流比F,并将其控制在一定范围内,便可提高分离效率这项研究对水力旋流器分离机理的认识与实际应用均有重要意义

To reduce the marking distortion , using the vertical 2 - dimension galvanometer distributing and f field lens focalize , the distance of the second galvanometer to the machining plane is the focus f . to avoid too hign temperature to distroy the resonance cavity because of too long marking time , cooling system and self - protector is used in the design

为减少打标畸变,采用垂直二维振镜分布, f物镜聚焦,将激光束经第二个振镜反射后到加工平面的距离为焦距f ,为了避免打标时间过长,温度过高破坏谐振腔,在设计中加上冷却系统和自我保护装置。

Starting with commercial available tosylmethyl isocyanide and but-2-enoic methyl ester, pyrrolo[2,1-f][1,2,4]triazine derivatives was prepared by twelve-steps process in total yiled of 5.0%~8% via pyrrole and pyrrolo [2,1-f] [1,2,4] triazine ring formation, substitution and amination reactions etc.

本文以简单的对甲基苯磺酰甲基乙腈和反-2-丁烯酸为原料,经过形成吡咯,吡咯并[2,1-f][1,2,4] 三嗪环,取代,酰胺化等等12步反应,合成了结构新颖的吡咯并[2,1-f][1,2,4] 三嗪衍生物,总收率达5%~8%。

The strains are arranged in order in terms of biological characteristics as follows: B_ 、B_5、 F-263、 B_1、 B_6、 B_f、 B_(y2)、 B_(12)、B_z、 B_9、 B_3、 B_(14)、 B_2、 B_4、 B_8、 B_(13)、 B_(y7)、 B_(11)、 B_(10)、 B_7、 B_(15); through comparing the mortality and muscardine rate to Monochamus alternatus larva of 21 strains, it is found that B_5、B_ and F-263 are superior to other strains.

比较生物学性状,结果从优到劣排序是:B_、B_5、F-263、B_1、B_6、B_f、B_(y2)、B_(12)、B_z、B_9、B_3、B_(14)、B_2、B_4、B_8、B_(13)、B_(y7)、B_(11)、B_(10)、B_7、B_(15);比较各菌株对天牛幼虫的致死率和白僵率,B_5、B_和F-263三个菌株优于其它菌株。

In this paper, 1554 strains of bacteria were isolated from the soybean rhizophere in the different continuous systems. Those biocontrol bacteria were screened in vitro and in vivo. 6 strains of them had the better control efficacy. They exhibited the different antagonism to the pathogens of soybean root in vitro. The strains 18BR2-2 and 18BR9-1 showed a wide range of antagonism against Fusarium oxysporum, F. avenaoeum, F. solani and Rhizoctonia solani in vitro. The 6 strains exhibited different control efficacy to soybean root rot in greenhouse and in field. In greenhouse, they could decrease the degree of soybean disease significantly, and the control efficacy of 18BR2-2 was the best and can reach 45.90%. In theory, soybean treated by 18BR2-2 may postpone the days of outbreaking of disease for 6 days. The strain 18BR2-2 also promoted the growth of soybean seedling and, raising the soybean height for 11.24%, increasing dry weight for 40.91% and fresh weight for 35.27%.

本研究从长期连作田种植的大豆及其根际土壤中分离细菌1554株,采用平板测试、温室盆栽及田间试验等方法,筛选了对大豆根腐病菌(主要是尖孢镰孢菌Fusarium oxysporum)有很好作用效果的生防细菌6株,在离体的条件下均表现出不同程度的拮抗作用,其中生防细菌18BR2-2和18BR9-1表现出很广的杀菌谱,对大豆根腐病菌尖孢镰孢菌(F.oxysporum)、燕麦镰孢菌(F.avenaoeum)、茄腐镰孢菌和立枯丝核菌都有很好的离体拮抗作用,并且这6株生防细菌在盆栽和田间的情况下均表现出对大豆根腐病一定的防治效果。

The possible reason is that doped Zr〓 ion enters the interstitial sites for its small ionic radius and no dipole complexes were formed. Monovalent ion F〓 has been reported to improve the scintillating performance greatly. Our results show that Agrand diagrams of F〓: PWO crystals obey an modified Cole-Cole equation. The crystals present similar dielectric relaxation phenomena to but have much higher activation energy than that of RE〓: PWO crystals, which originate from the 〓 dipole complexes. The co-doping of trivalent Y〓 and pentavalent ion Nb〓 does not always show a better scintillation performance than that of solely doped samples, which could be attributed to the existence of the 〓 dipole complexes, which counteract the solely doping effect.

而Zr〓由于离子半径太小,可能进入间隙位置,不形成类似的偶极缺陷;一价负离子F〓的掺入提高了晶体闪烁性能,阻抗谱测试结果表明F〓:PWO晶体Argand图符合修正的Cole—Cole方程,晶体同样表现出介电弛豫现象,并具有比RE〓:PWO晶体中高得多的弛豫激活能,可能起源于形成的〓偶极缺陷;Y〓和Nb〓双掺的PWO晶体并没有显示出两种离子单掺优点的叠加,阻抗谱测量结果表明,在双掺晶体中可能形成〓偶极缺陷,使两者掺杂作用相互抵消。

In 1959 Sauerbrey developed an empirical equation to describe the mass sensitivity of gas phase mass deposition on a piezoelectric quartz crystal——Sauerbrey equation:ΔF=〓=-CΔm, where ΔF is measured frequency shift, in Hz; F〓 is the fundamental resonant frequency, in Hz;Δm is mass change, in g; A is piezoelectrically active area , in cm〓;μ〓 is shear modulus of quartz, 2. 947×10〓g· cm〓;ρ〓 is density of quartz, 2. 648g·cm〓; C is mass sensitivity constant , in 〓.

1959年,Sauerbrey最先提出了将压电晶体谐振器用作定量测量质量的装置,并从理论上导出了频率与质量变化之间的关系式——Sauerbrey方程:ΔF=〓=-。。。

The amount of radiopacity promoted by rDBM/f and rhFGF-1/f at 8 weeks was equivalent and was greater than antecedent times.

在8个星期rDBM/f和rhFGF-1/f促进了的防辐射的数量是等价物并且是伟大的与比先行的时间。

Compared to previous approaches on the same MEDLINE abstracts, the presented resolution was promising for its 92% F-Score in pronominal anaphora and 78% F-Score in sortal anaphora.

与其它方法在相同语料做比较,所提的方法处理指代词指代现象可达到92% F-Scorec和名词指代现象可达到78% F-Score。

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推荐网络例句

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力