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With western blot analysis and DNA polymerase chain reaction-single strand conformation polymorphism assay,protein product of Rb gene and mutation in exon 5～8 of P_(53) gene were examined in 17 cases of human testicular seminoma.

采用Western印迹和DNA聚合酶链反应－单股构造多态性分析法对17例人睾丸精原细胞瘤组织标本的抗癌基因Rb表达的蛋白产物和P_（53）基因外显子5～8的突变进行检测，发现有3例标本的Rb基因表达的蛋白产物缺失，有4例标本的P_（53）基因有点突变，对照的正常人睾丸组织和视网膜组织均呈Rb基因蛋白产物表达阳性，对照的正常人睾丸组织中未发现P_（53）基因突变。

Some characteristics of the novel gene YH1 was explored by bioinformatics. The YH1 gene was mapped on the chromosome 20 with the size of 7. 5 kb or so by blasting to genome database and this fragment size for YH1 gene coincided with the result of Souther blot. There were nine exon and eight intron.

利用生物信息学方法，在基因组数据库中比较，发现YH1基因存在于一个定位在20号染色体上7.293kb的DNA序列中，这与Southern blot结果相符。9个外显子被8个内含子隔开。

The frequency of losing exon 5-8 was the highest . It was consistent with the results of the FHIT gene whole length cDAN by RT-PCR.Conclusions: The above results showed that the changes of FHIT gene is probably related to the development of kinds of tumors, which is an important molecular event. FHIT gene can be considered to be a tumor suppresser gene, our studys provided a theoretic and experimental data for further research on the function of FHIT gene.

结论本研究结果初步证明FH工T基因改变可能与多种肿瘤的发生有关，是肿瘤发生的一个重要的早期分子事件，支持其作为一个抑癌基因在肿瘤发生中发挥作用，为进一步深入研究FH IT基因的功能提供了理论和实验依据。

Object: 1. To study the correlation between the total serum IgE and AD. 2. To detect the possible association between SNPs -137,-607 in promoter region 1 of exon 2 of IL-18 and AD patients in Chongqing.

目的：1、分析血清总IgE与特应性皮炎临床相关性 2、分析IL-18基因外显子2上游启动子1第-137位点和-607位点基因多态性与重庆地区AD患儿的相关性。

Methods The polymorphism at nucleotide 307 in the 4th exon of HCR gene was detected in 118 patients of the Uigur population with psoriasis vulgaris and in 127 normal controls.

方法测定118例新疆维吾尔族寻常性银屑病及127例正常人对照组的HCR基因第4外显子第307位点基因多态性。

Using a pair of special primers to amplify the exon 3 of Myostatin gene in Northeast Wapiti, we aimed to detect the polymorphisms of Myostatin gene.

应用pcr-sscp和测序的方法对东北马鹿Myostatin基因的第3外显子进行了多态性分析。

Methods By real-time PCR, the damages of exon 5 and 8 of p53 gene were probed in 37 workers exposed highly to, 16 manager and logistic employees exposed less to an arsenic mill in Yunnan province, and also 25 local people who did not contact with any white arsenic in near past time. At the same time, the urinary total and organic arsenic of workers were detected.

选择云南某砒霜厂－线工人37例、管理和后勤人员16例和当地近期无毒物接触人员25例为研究对象，应用实时荧光定量PCR检测人群p53基因第5和第8外显子损伤，同时检测尿中总砷和有机砷含量，评价人群对砷的代谢转化与p53基因损伤的相关性。

The partial sequences of exon Ⅰ of hormone-sensitive lipase genes in yak, cattle, zebu, and buffalo were analyzed. Comparisons of these sequences and the deduced amino acid sequences with the homologous HSL gene and protein sequences in other mammalian species including pig, human, mouse, and rat retrieved from the GenBank were carried out and finally a phylogenetic tree was constructed using the partial DNA sequences of the HSL genes in all species.

在对牛科中4种动物即牦牛、瘤牛、普通牛和水牛HSL基因外显子Ⅰ部分核苷酸序列进行测定的基础上，与Gen- Bank中其他物种相应基因核苷酸序列、氨基酸序列进行了比对分析，并构建了牦牛与其他物种间分子系统进化树。

Three combinant retrovial expression vectors, aM1, aM2 and aM3, which was respectively loaded with reverse fragment of c-myc exon 1, 2 and 3, were transferred into rat arterious smooth muscle cells and assayed 1 month after their stable expression.

将分别载有c-myc第1、2和3外显子反义片段的三种重组逆转录病毒表达载体aM1、aM2和aM3导入大鼠主动脉平滑肌细胞，并于持续筛选后1个月进行稳定表达检测。

Methods Three recombinant retroviral expressive vecto rs, aMl, aM2 and aM3, which respectively loading reverse fragment of exon 1,2 and 3, were transferred in to rat arterious SMC and assayed 1 month after their stable expression.

分别载有c-myc 第1、2 和3外显子反义片段的3种重组逆转录病毒表达载体 aM1、aM2 和 aM3 被导入体外培养的大鼠主动脉平滑肌细胞，并于持续筛选后1个月进行稳定表达检测。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。