查询词典 Edison effect

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

Great inventor Thomas Edison's mother, Nancy Edison's education to cultivate, have an unique: Edison teacher teaching method by instilling, Edison very antipathetic.

大发明家爱迪生的母亲南希，对爱迪生的教育培养，有不省独到之处：爱迪生的老师采用灌输法教学，爱迪生极为反感。

This phenomenon was called the Edison effect, become electron tube and electronic industries. 1887, from Xiaolan government, and in the same year in a larger city, the laboratory equipment is also updated the famous Edison Laboratory (later known as the invention factory).

这种现象后来称为爱迪生效应，成为电子管和电子工业的基础。1887年，移居西奥兰治，并于同年在该市创建规模更大、装备也更新的实验室，即著名的爱迪生实验室。

Edison bulbs in a vacuum test, accidentally discovered the cold, there is a current hot electrode. This phenomenon was called the Edison effect, become electron tube and electronic industries.

电照明的实现，不仅大大改善了人们生产劳动的条件，也预示着日常生活电气化时代即将到来。1883年，爱迪生在试验真空灯泡时，意外地发现冷、热电极间有电流通过。

In 1883 Edison had discovered the thermionic emission phenomenon, alsois called "the Edison effect", namely nearby the metal surface partialelectrons or the ion enable its non- regular movement because of thehigh temperature to obtain the enough kinetic energy, overcomessuperficial the fetter, is leisurely outside the metal.

1883年爱迪生发现了热电子发射现象，也叫&爱迪生效应&，即金属表面附近的部分电子或离子因高温而使其无规则运动得到足够的动能，克服表面的束缚，逸出金属之外。

How Americans Showed Their Respect for Edison Thomas Alva Edison was awarded more patents on inventions than any other American.

美国人怎样表达他们对爱迪生的敬意托马斯·阿尔瓦·爱迪生获得的发明专利，超过了任何一位美国人。

A clever boy and a thickheaded child to oral exam, the teacher ask clever boy,"Who invented the electric light,""Edison" and "who discovered the ray","Madame Curie", who discovered the earth gravity ",","100 points, Newton, intelligent children to help the answer thickheaded child told him, the teacher asked," Who is your dad,""," your mama Edison who is ","Madame Curie","who told you,""Newton".

一个聪明的孩子和一个笨孩子去考口试，老师问聪明的孩子；"谁发明了电灯"，"爱迪生"，"谁发现了雷"，"居里夫人"，谁发现了地球有引力"，"牛顿"，100分，聪明的孩子为了帮笨孩子把答案告诉了他，老师问；"你爸是谁"，"爱迪生"，"你妈是谁"，"居里夫人"，"谁告诉你的"，"牛顿。

Along with Thomas Edison's carbon button initiated the foundation principles upon which the diode was later invented, and upon which radio, television, and computer transistors are based through the foresights of our beloved Thomas Alva Edison who in his lifetime was an inventor of beggaring imagery.

与Thomas Edison 的碳一起按钮创始二极管以后被发明的基础原则，并且在哪台收音机，电视，并且计算机晶体管根据通过在他的一生是beggaring 的成像的发明者我们心爱的Thomas Alva Edison 的foresights 。

The Edison 20 yearsold raise one's head start to study the electric lamp, the lasted morethan 10 years, he has successively selected the bamboo cotton andkapok, the graphite, the tantalum...... And so on over a thousandkind of different matter make the lamp silk material to carry on theexperiment, often passes 霄 until dawn, some he and assistantsunexpectedly continuous working 5 day and nights. In 1879 Edison withthe carbon silk took the incandescent lamp silk, and lights for 40hours.

爱迪生20岁出头开始研究电灯，历时10余年，他先后选用了竹棉、石墨、钽……等等上千种不同物质作灯丝材料进行试验，时常通霄达旦，有一次他和助手们竟连续工作5昼夜。1879年爱迪生用碳丝作为白炽灯丝，并点燃40小时。

Some one asked Edison :"you have experimented on over one thousand materials, but they all turn out unfit as lamp filament ,what is the meaning of your experiment " Edison answered:I come to know

有人问爱迪生，你试验一千多种材料，结果是都不能做灯泡的灯丝，你的试验有什么意义？

You can do some assertiveness training.

你可以进行一些自信训练。

We were well on the path to making a rear-wheel-drive global platform," says Mays."

我们正致力于建立一个后轮驱动的平台，"Mays这样说道。"