查询词典 EST

We established the technical system to obtain the EST library of plant specific chromosome,which is important to gain the ESTs and genes located on specific chromosome and provide a new tools for complicated allopolyploid genome research.

本项目获得了建立植物特定染色体EST文库的技术体系，它对分离特定染色体的EST及克隆新基因有重要参考价值，也为异源多倍体植物基因组研究提供了新的技术手段。

Results showed that tissue-specific distribution was found in each electrophoresis pattern of EST, GDH IDH and POD, and EST showed a distinct dissimilitude between intersexual samples.

结果表明，乌苏里拟鲿EST、GDH、IDH、ROD同工酶语中存在不同程度的组织特异性，EST表现出明显的性别差异性。

Further studies should be done to confirm if Est-4, Acp-2 and Acp-3 could regulate the development of 8-nucleat embryo sac.

授粉后Est和Acp的酶位点变化说明与胚珠发育相关的一系列基因表达有时序性，位点Est-4、Acp-2和Acp-3是否直接参与了八核胚囊发育的控制，有待于进一步的研究。

And the repression of NO-induced cell death with Ppbi-1 overexpression in transgenic lines was apparent even at SNP concentrations up to 1mM.In addition work,we found that most of suspension cells treated with 0.5mM SNP can be stained by Sytox green,some PCD morphologic characteristics such as chromatin condensation and margination can be observed.DNA Ladder was also detected with these cells.As to the control and the cell treated with 0.5mMSNP+20μM Est,the above-mentioned characteristics can\'t be detected.

对0.5mM SNP和0.5mM SNP+20μM Est处理的飞廉转基因细胞培养三天后用特异性核染料sytox染色，0.5mMSNP单独处理的细胞大部分核被染色，而且细胞核出现了核凝聚、以及染色质边集等凋亡细胞的特征；提取细胞DNA电泳分析，观察到了DNA LADDER，这说明，该细胞可能已经发生凋亡，而Est诱导Ppbi-1基因表达的细胞均未出现这些凋亡特征。

(1)29,28,26 of 30 pairs of EST-SSR primers from wheat respectively had amplification products in maize,oilseed rape and soybean,the rate of amplification was 96.7%,93.3%,86.7%,respectively;12 of 30 pairs EST-SSR primers from wheat showed polymorphism in monocotyledons wheat and maize,9 in dicotyledons oilseed rape and soybean,and 4 in both monocotyledonous and dicotyledonous crops.

基金项目：国家生命科学与技术人才培养基地科技创新基金项目(2007088)；国家油菜产业化项目科学家岗位"化杀岗位"（nycytx-00505）；国家自然科学基金项目(30871578)中文摘要：探讨单、双子叶作物间EST-SSR引物和标记的通用性。选取30对小麦、8对油菜和14对白菜的EST-SSR引物，分别以10个小麦、10个油菜、5个大豆品种和5个玉米自交系的基因组DNA为模板，进行PCR扩增及扩增产物的多态性分析。

OPJ16-759 shows 83% to 100% identity with 14 EST sequences from abiotic stressed leaves of Vitis vinifera var. Chardonnay, 94% to 100% identity with 3 EST sequences from water stressed berry of Vitis vinifera var. Cabsau.

OPJ16-759与欧洲葡萄"霞多丽"叶片14条非生物胁迫EST序列有83 ~ 100%的同源性，与欧洲葡萄"Cabsau"浆果3条水分胁迫的EST序列有94 ~ 100%的同源性。

In the research, one kind of guzmania species named ostara was employed to be as material, and the full-length cDNA plasmid library of floral organ was constructed successfully by using SMART technology. The library had 3×10^6 original titer and more than 1 kb insert fragments. After 5'EST sequencing from 2004 positive clone chosen at random and clustering analyse, 1 758 high-quality sequences and 1 365 unigenes including 175 contigs and 1 195 singlets were obtained. These unigenes had 1 283 valid ORFs. COG analysis showed that those proteins coded by EST sequences were divided into 22 classes. Through blast analysis, full length or part cDNA of some genes controlling flower color, development of floral organs, florescence regulation and other breeding value genes were obtained.

本研究以擎天凤梨属品种Ostara为材料，采用SMART技术成功构建了擎天凤梨花器官的质粒型全长cDNA文库，初始文库滴度为3×10^6，插入片段平均长度大于1kb；随机挑取2004个阳性克隆进行5'EST测序，获得高质量序列1758条，经拼接获得1365条单基因簇，其中跨叠群175个和单条序列1195个；经ORF寻找共获得有效ORF1283条；经COG分析EST序列编码的蛋白质被分为22类；经Blast分析后，获得一批花色、花器官发育、花期调控以及其它育种价值基因（包括cDNA全长或片段）。

The conditions to measure accurately and the principles to select parameters of the multi— probes EST method are proposed, and the theoretical research, computer imitation and the experiment on a machine tool prove that these principles and suggestion are necessary and effective. The influence of the initial value errors of the probes, the installation error of the probes system, and the remaining error caused by the radical error motion are analyzed quantitively on the measurement accuracy of the multi—probes EST method. The influence of the error caused by the probe's axes which is not in a plane and the perpendicularity error of the plane which the probe's axes is in to the stational axis are discussed on the measurement results.

结合理论分析，对这些EST方法进行了计算机仿真分析和研究，重点研究了这些方法的应用条件和误差分离精度，所得出的结论是：三测头法EST基本上能实现各项误差的完全分离，适于在位测量使用；二测头法在恰当地选择测头安装角的条件下，可以得到较高测量精度，适于大型零件圆度、圆柱度、径向圆跳动和全跳动的在位测量；对置两测头时域法可以在一定程度上改善单测头法测量精度，但对随机径向误差无能为力；对置两测头频域法存在严重的谐波损失。

They conta i ned full genes ORFs and proved to have been prolongated effectively. The result showed that the seqences of 14 genes were genes known previously and 6 genes were new .We separately chose one seqence from known and the other from unknown genes to make full-length cDNA annotation. Their code number was EST contig110 and EST contig133. We designed specific primers to amplify the two genes by RT-PCR, and then sequenced. The result was obtained as expected and proved that silico cloning genes was reliable.

对20条contigs进行了基因注释，证实它们均得到了有效延伸，具有完整开放阅读框，其中已知的猪基因序列14条，猪新基因序列6条，分别从已知猪基因序列和未知猪基因序列中各抽取一条感兴趣序列进行全长cDNA的预测，它们的编号分别为EST contig110和EST contig133，设计特异引物对两条候选序列进行克隆与测序，结果与电子克隆所得序列一致，证实了电子克隆的有效性。

OPJ16-759 shows 83% to 100% identity with 14 EST sequences from abiotic stressed leaves of Vitis vinifera var. Chardonnay, 94% to 100% identity with 3 EST sequences from water stressed berry of Vitis vinifera var. Cabsau.

OPJ16-759与欧洲葡萄&霞多丽&叶片14条非生物胁迫EST序列有83 ~ 100%的同源性，与欧洲葡萄&Cabsau&浆果3条水分胁迫的EST序列有94 ~ 100%的同源性。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。