查询词典 ELISA

Routine detecting techniquefor cell immunity such as white blood count and differential, lymphocyte blastogenesis test has beenused, and combine with flow cell technology to detect the level of cell immunization after advancedimmunization at several important aspect, and tracking experiments to observe changes in the numberof the WBC and lymphocytes, lymphocyte proliferation, the dynamic changes of CD4~+ and CD8~+lymphocyte subsets andγ-IFN; and detected dynamic changes rules of IgG, IgM in humor andmucosa with indirect ELISA, then compare with ordinary immunization methods.

主要采用白细胞计数和分类计数、淋巴细胞转化试验等常规细胞免疫检测技术结合先进的流式细胞技术从细胞免疫的几个重要方面入手检测猪瘟超前免疫后细胞免疫的水平，并进行跟踪试验，观察其白细胞、淋巴细胞数目变化情况，淋巴细胞增殖情况，CD~(4+)和CD~(8+)淋巴细胞亚群及γ-IFN的动态变化规律；同时通过间接ELISA方法检测体液和黏膜中各IgG、IgM的动态变化规律，同时与普通免疫方法进行比较。

The kinetics of T-cell subsets, blastogenic responses and anti-NDV serum IgG antibody of La Sota-immunized chicken were investigated using FACS, blastogenesis microassay and indirect ELISA after the chickens were thymusectomized.

然后，在不同的日龄分别用FACS、MTT及间接ELISA测定脾脏和外周血中CD4+和CD8+T淋巴细胞亚类数量、脾T淋巴细胞转化功能及病毒特异的血清IgG抗体的动态变化。

This study provide a way for the ELISA detection of botrytis cinerea.

该研究为今后的ELISA法快速检测灰霉菌提供了一些参考依据。

Coli BL21 and purified. The binding activities of immune sera to three EDs were examined with ELISA and Western blotting analysis.②Asthma model was induced with ovalbumin in mice of vaccine group, control vector group and asthma group. Airway pressure-time index and number of cells in BALF were checked. The quantity of goblet cells in bronchiole and the level of MUC5AC mRNA in lung were investigated by PAS staining and RT-PCR assay.

采用ELISA和Western blotting法检测免疫血清抗体对ED的亲合力。2、对DNA疫苗组小鼠制备OVA致敏哮喘模型，检测气道压力峰值—时间指数、支气管／肺泡灌洗液中炎性细胞数量，PAS特染观察小支气管杯状细胞数量及RT-PCR法检测肺组织中粘蛋白MUC5AC mRNA水平，免疫组化和RT-PCR法检测GM-CSF的蛋白与mRNA水平。

Objective To develop a high specific and sensitive ELISA for human brucella antigen detection.

目的 建立一种高特异性、敏感性的 ELISA 方法检测人布鲁氏菌抗原。

A high specific and sensitive sandwich ELISA for human brucella antigen detection has been successfully developped.

建立了高特异性、敏感性的检测人布鲁氏菌抗原的ELISA方法。

Coli were purified and used as the coating antigen in indirect ELISA test, and the results were compared with those obtained by the traditional tube agglutination test method in 100 serum samples in general survey for the Brucella infection.

以大肠杆菌表达、纯化的BP26和OMP31重组蛋白为包被抗原，初步建立了检测血清特异抗体的间接ELISA方法，并以现地牛布氏杆菌普查检测血清100份为样本，与传统的试管凝集试验进行了比较研究。

There is neither paper about detecting DsMV by DBH or IC-RT-PCR nor report about comparing ELISA with these methods published before. Therefore, the DsMV detection methods developed in this study can be applied to virus certification scheme of calla lily and help to produce healthy seedlings in Taiwan.

之前未曾有以DBH或IC-RT-PCR检测DsMV之报告，或这些方法与ELISA比较的相关报导，因此希望本研究所研发的DsMV检测方法未来可应用於每芋种苗病毒验证作业，以协助国内业者生产健康海芋种苗。

Aim:To obtain sufficient Epstein-Barr viral capsid antigen and apply it to the diagnosis of Nasopharyngeal Carcinoma based on ELISA.

目的：获得足够的EB病毒壳抗原，建立ELISA方法用于鼻咽癌的诊断。

Conditions of the ELISA were optimized, and suggested that the optimistic concentration of the recombinant capsid protein was 6.4,.

通过对已知PCV2血清及其他病毒血清的试验，表明该ELISA方法具有良好的特异性。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。