查询词典 ELISA

Objective To examine the expression of glucose-6-phosphate isomerase in patients with rheumatoid arthritis, and explore its diagnostic value. Methods The GPI was detected by ELISA in 116 cases of RA, 50 cases of suspected patients from RA, 114 non-RA and 60 cases of normal control.

目的 检测6-磷酸葡萄糖异构酶抗原在类风湿关节炎中的表达情况，探讨其在RA中的诊断价值及临床意义。

Isotypes, titer and affinity of the Mabs belong to G1 subclass withκlight chain.

利用ELISA试剂盒对单抗效价、亚类、亲和常数进行分析，4株单抗都属于IgG1，轻链为κ链。

The isotypes of the five anti-CT mAbs were identified as IgM heavy chain and λ light chain. For the purification of anti-CT mAb, Hitrap IgM Purification Column was used.

其中CT-3A与CT-9A单株抗体经大量生产以Hitrap IgM Purification Column纯化后，经间接型ELISA免疫分析抗体效价在1：15，625。

To prepare the phosphorylated PRAS40 (Ser183) antibody, We chosen 10-amino acid including Ser183 as antigen peptide through antigenicity and hydrophobicity analysis, hinged on keyhole limpet hemocyanin, and used the KLH-peptide to immunize rabbits. After antibody serum titer detection by enzyme linked immunosorbent assay, the antibody was purified with rProtein A sepharose fast flow and dephosphorylated antigen membrane. The antibody titrate reached 1:10 000 after purification and its special property was enhanced with absorption treatment of dephosphorylated antigen membrane. In addition, we used rabbit anti-PRAS40 antibody and the phosphorylated PRAS40 (Ser183) antibody to detect PRAS40 expression in several cell lines, including the normal cells HL7702, HEK293, tumor cells HepG2, A549 and S180. There were no quite difference among these cells; otherwise, we observed the decreased phosphorylation level of Ser183 after amino acid withdrawal treatment.

为了制备PRAS40(Ser183)磷酸化多克隆抗体，本实验通过蛋白疏水性抗原性分析设计多肽抗原，用其免疫家兔获得抗血清， ELISA检测其效价为1：10 000； Western blotting法检测发现，通过rProtein A Sepharose亲和层析纯化并经非磷酸化的抗原条吸附处理后的抗体可以明显提高磷酸化抗体的特异性；用PRAS40抗体及PRAS40(Ser183)磷酸化抗体对正常细胞HL7702、HEK293及肿瘤细胞HepG2、A549、S180的检测显示：磷酸化的Ser183在不同细胞中表达差异不显著，而在经细胞饥饿处理的HEK293细胞中却明显观察到了S183磷酸化水平随氨基酸含量降低而减弱的现象。

Sera from 18 Chinese haemophiliacs treated with factor Ⅷ produced in USA from 1982 to 1984 were tested for antibody to LAV/ H T L V-Ⅲ by ELISA. Four patients ( 22.2%) showed strong positive results which were confirmed by immunofluorescence test and Western blot,but none of them has developed symptoms related to AIDS.

对浙江省1982～1984年注射了美国产浓缩Ⅶ因子制剂的18例血友病患者，用酶联免疫吸附法检测了血清中淋巴腺病病毒/人T细胞Ⅲ型病毒抗体，发现4例阳性，并经免疫荧光试验和Western印迹法证实。2例应用了国产浓缩Ⅷ因子者抗体阴性。

Construct PLXRN-IL-1Ra and PLXRN-IL-10 vector, lapine synoviocytes were first transduced in culture by retroviral infection. The genetically modified synovial cells were then transplanted by intra-articular injection into the knee joints, assay of joint lavages confirmed that the gene expression was not lost after 14 days of transfer. Knees receiving the hIL-1Ra had significantly reduced cartilage breakdown. Delivery of the hIL-10 was less effective, When both genes were used together, there was a greater inhibition of cartilage breakdown and a considerable reduction of cartilage matrix degradation.

构建PLXRN-IL-1Ra、PLXRN-IL-10逆转录病毒载体，体外感染同种异体原代滑膜细胞，接着将转染了外源基因的滑膜细胞行关节腔注射入创伤性骨关节炎兔膝关节，通过对关节滑液的ELISA分析证明外源基因的表达在基因转移后14天还很稳定，只接受hIL-1Ra基因治疗的膝关节软骨损坏明显减轻，接受hIL-10基因治疗的膝关节治疗也有一定效果，当两种基因同时导入时，有非常明显的抑制软骨破坏和软骨基质降解的作用。

In this study, DAS-ELISA was used to detect PRSV in the leafages of the samples, which were collected in Haikou city, Fusui city, Zhengzhou city, Zhangzhou city.

以海口、扶绥、郑州、漳州等地采集回来的叶片样品为材料，首先通过双抗体夹心酶联免疫吸附分析的方法对所采集的叶片样品进行检测。

Methods Phosphotyrosine was first combined with hemocyanin from keyhole limpets. Then, rabbits were immuned with the complete antigen and antisera were gained. The ELISA system consisted of purified antiphosphotyrosine antibody as first antibody and sheep antirabbit lgG-HRP as second antibody. Results CV within the batch was 8.4%.

应用合成的磷酸酪氨酸-钥孔帽贝血蓝蛋白免疫家兔得到抗血清，将纯化的抗磷酸酪氨酸抗体作为第一抗体，羊抗兔Ig酶结合物作为第二抗体，建立双抗体夹心酶联免疫吸附测定法。

Elisa, squatting on the ground, watched to see the crazy, loose-jointed wagon pass by.

伊利莎蹲在地上，看着这辆怪模怪样、松松垮垮的马车驶过去。

Blocking heart lymph of group E and plasma of the rabbits before operation and at 3, 7, 14, 30, 60 days after operation, PICP and PIIINP were measured by ELISA.

阻断实验组动物心脏淋巴循环，用原位杂交的方法测定各个观察时期Ⅰ、Ⅲ型胶原蛋白mRNA的表达。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新