查询词典 ELISA

Haemocyte membrane of Fenneropenaeus chinensis was purified and WSSV was labeled with DIG, the DIG-WSSV was incubated with MAb and the mixture was added to ELISA plate coated with membrane haemocyte, then the binding was detected with anti-DIG antibody.

提取中国对虾血细胞膜并对WSSV进行地高辛标记，将病毒和各单抗混合作用后吸附对虾血细胞膜，使用抗地高辛抗体检测结合到血细胞膜上的病毒。

Expression analysis with RT-PCR revealed that PcLGBP gene was exclusively expressed in hepatopancreas and the expression could be up-regulated by heat-killed Listonella anguillarum. The binding specificity of PcLGBP to ligand were subsequently examined by recombinant expression of the cDNA fragment encoding its mature peptide in Escherichia coli BL21 (DE3) and ELISA approach.

为分析PcLGBP基因的功能，通过半定量RT-PCR方法研究了其在组织中的分布，并用荧光定量PCR技术分析了其对细菌刺激的响应，结果表明PcLGBP基因是肝胰腺特异表达的基因，能响应热致死鳗弧菌刺激而增强表达，说明PcLGBP基因可能参与螯虾的细菌防御反应。

In mouse peritoneal macrophages. Methods Mouse peritoneal macrophages were cultured in the presence of heat-killed yeast-phase PM for 24 h, and the average fluorescence intensity of TLR-2, TLR-4, and Dectin-1 in the macrophages was detected using flow cytometry. Fluorescent staining of the macrophages was performed to observe the fluorescence of TLR-2, TLR-4, and Dectin-1 with confocal microscopy. TNF-α mRNA in the cell culture supernatant was measured with real-time PCR, and TNF-α protein detected using enzyme-linked immunosorbent assay.

马尔尼菲青霉酵母相菌液与小鼠腹腔巨噬细胞共培养24h，采用流式细胞技术检测巨噬细胞TLR-2、TLR-4及Dectin-1的平均荧光强度；共聚焦显微镜观察荧光染色的受体；ELISA法测定培养液上清中TNF-α的浓度；Real time PCR检测不同时间段TNF-α的mRNA表达。

Objective To study the effects of heat-killed Penicillium marneffei on the expressions of toll-like receptor-4 (TLR-4),toll-like receptor-2 (TLR-2) and dendritic cell associated C-type lectin-1 (Dectin-1)and the production of the proinflammatory cytokine tumor necrosis factor-α.

办法马尔尼菲青霉酵母相菌液与小鼠腹腔巨噬细胞共培养24h，采用流式细胞技术检测巨噬细胞TLR-2、TLR-4及Dectin-1的平均荧光强度；共聚焦显微镜观察荧光染色的受体；ELISA法测定培养液上清中TNF-α的浓度；Real time PCR检测不同时间段TNF-α的mRNA表达。

It was used to detect telomerase activity in 293 cells and RNase-pretreated or heat-treated cells as control.

与常规TRAP法相比较，应用端粒酶TRAP-ELISA法检测端粒酶阳性的293细胞和阴性对照标本(加热或RNase处理和正常人内皮细胞)。

Methods IUGR model were established by maternal protein-malnutrition. The mRNA levels of TLR4 in hepatics of IUGR rats at 3 weeks of age were analyzed by fluorescent quantization RT–PCR. TLR4 protein levels in hepatics were determined by Western blot. Hepatics TNF-αand IL-1βlevels were measured by ELISA.

采用孕期蛋白质营养不良法建立IUGR大鼠模型，应用荧光定量RT-PCR技术检测3周龄子鼠肝脏中TLR4的mRNA含量变化，Western blot方法检测子鼠肝脏TLR4的蛋白表达，并应用酶联免疫吸附法检测肝组织中肿瘤坏死因子-α和白介素-1β的水平。

It was purified by immobilized metal ion affinity chromatography under native conditions because there was a 6 histidines tag at its amino acid terminus.

在间接ELISA和免疫印迹试验中，重组的基质蛋白可与马传贫阳性血清样品发生反应，而与健康马血清无任何反应。

Methods: Bypreparing anti-human type Ⅳ collagen IgG with rabbits and utilizing sodium periodate horse-radish peroxidase-labelling anti-human type Ⅳ collagen monoclonal antibody to change diluentcomponent and concentration, we establioshed sandwich ELISA.

以免疫家兔制备抗人COLⅣ多抗包被微孔板，采用改良过碘酸钠法辣根过氧化物酶标记抗人COLⅣ单抗，改变各种稀释液成分及浓度，建立双抗夹心酶免疫测定法。

Following that, the hybridoma cells were injected into the peritoneal cavity of BALB/c mouse.

间接ELISA法测定抗体的效价，Western-blot检测抗体的特异性。

The property of the hybridoma cell lines to secrete expected antibody was confirmed by ELISA and Western blotting analysis.

杂交瘤细胞产生的抗体可以作为相关实验的材料，杂交瘤细胞也可作为从事植物抗体研究时抗体基因的来源。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。