查询词典 ELISA

Draining lymph nodes isolated from the BALB/C mice immunized with rabbit skin collagen Type Ⅲ accord- ing to a rapid immunization protocol, the lympho- cytes were fused with the myeloma cell line NS-1, 3 out of total of 201 growing hybridomas we obtained were producing collagen Type Ⅲ reactive antibodies after 3 limited dilution clones performed.

本实验采用快速免疫的方法用兔皮肤Ⅲ型胶原免疫BLAB/C小鼠，取淋巴结的细胞与小鼠骨髓瘤细胞NS-1融合，240孔中201孔有杂交瘤生长，用ELISA检测有6孔反应阳性。选3孔生长良好，抗体浓度高的细胞进行有效的有限稀释法克隆3次，获3株分泌抗Ⅲ型胶原单克隆抗体的杂交瘤细胞。其中二株属IgG2b，一株为IgG1。

The serum and CSF levels of IL1β, IL6, TNFα and IL1ra in the patients with different kinds of dementias and the controls were measured using ELISA.

用夹心式酶联免疫吸附法测定各组老年人血清和脑脊液中白介素1β（IL1β），白介素6（IL6），肿瘤坏死因子α和白介素1受体拮抗剂（IL1ra）的水平。

The fusion DNA fragments of ag85b-mpb64 and ag85b-mpb64-esat-6 were obtained by PCR andSOE technique. Various DNA vaccines were constructed with the pcDNA3.1: fusion of two genes, and of three genes, bivalent combinations and trivalent combinations(pCA+pCM+pCE6). BALB/c mice were vaccinated with this DNA vaccines.The mice injected withBCG were positive control and the mice injected with pCDNA3.1 and PBS were negative control.The mice were immunized 3 times with 2-wk intervals. The animals in group BCG were only inoculatedsubcutaneously with 1×10~6 CFU BCG at initial vaccination. The serum IgG titers and IgG isotype weredetermined using iELISA coated with M. bovis PPD and rMAE protein expressed and depurated inprokaryotic expression system every week.

同样，利用PCR和SOE技术，获得牛分枝杆菌mpb64-ag85b和mpb64-ag85b-esat-6融合基因，以pCDNA3.1为载体构建了牛分枝杆菌多价组合和多基因融合DNA疫苗：二基因融合(pCDNA3.1-MPB64-Ag85B，简称pCMA)和三基因融合(pCDNA3.1-MPB64-Ag85B-ESAT-6，简称pCMAE)DNA疫苗；二价组合和三价组合(pCA+pCM+pCE6)DNA疫苗，免疫BALB／c小鼠，以牛分枝杆菌BCG免疫组为阳性对照，以pCDNA3.1及PBS免疫组为阴性对照，共免疫3次，每次间隔2周，BCG组仅初免时皮下免疫1次。1免后每周，以原核表达纯化的重组MPB64-Ag85B-ESAT-6蛋白和牛分枝杆菌PPD为包被抗原，以间接ELISA方法检测血清IgG水平及lgG亚类。

PCR HBV-DNA method can be Used in this research to evaluate the disinfectant effect of the endoscopes in Huangshi city.

PCR HBV-DNA对与内窥镜中痕量的HBV的检测试验方法优于ELISA HBsAg方法，在本次调查研究中应该采用PCR HBV-DNA的检测方法。

Although the virus coat protein was present in the endosperms of the seeds of infected wisteria plants by ELASA tests, it is not seed-borne.

ELISA分析显示此分离物与BCMV和WMV间具有血清学关系。

The concentrations of serum SIL-2R of 158 epileptics were measured with ELISA.

目的 探讨SIL-2R在癫痫患者血清中含量及临床意义。

Methods CCL20 and HBsAg eukaryon expression vector was constructed by using DNA recombinant technology.

方法采用基因重组技术，构建趋化因子CCL20和表面抗原的真核表达载体，将重组载体用肌肉免疫的方式分别注射正常的C57BL/6小鼠，通过ELISA方法检测C57BL/6小鼠的抗-HBs抗体水平、细胞增殖情况。

The serological assay for 37 garlic field samples in HeiLongJiang showed that the infection rate of LYSV、GCLV、OYDV、ShLV and PVX was 95%、86%、74%、50% and 78% respectively and most of them were complex infected.

对黑龙江省大蒜病毒病原进行调查及DAS-ELISA检测结果表明：37份大蒜田间样品中LYSV、GCLV、OYDV、ShLV和PVX的检出率分别为95%、86%、74%、50%和78%，且多数为复合侵染。

Plasma insulin,glucagon and cortisol levels were measured by radioimmunoassay,and the levels o...

用放免法测定血中胰岛素、胰高血糖素和皮质醇的浓度，用ELISA的方法测定血中IL 6和TNF α的浓度。

The concentration of PA1b in several plants including pea, taro, ginger, tomato, bitter gourd, garlic, onion, towel gourd and cucumbers was measured by competitive inhibiting enzyme-linked immunosorbent assay method to study the role of pea albumin 1b(PA1b) during the development of plants.

为研究豌豆胰岛素PA1b在植物生长发育中的作用，利用竞争抑制ELISA法，检测PA1b在豌豆、芋头、姜、西红柿、苦瓜、蒜头、洋葱、丝瓜、黄瓜等植物中的分布，以及PA1b在豌豆种子发芽过程中的含量变化。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。