查询词典 ELISA

MethodsA phage random twelve peptide library was screened 4 times by monoclonal antihuman FGF7. ELISA staining examination was performed to choose monoclonal phages at random. Also, DNA extracted from phages with better bindingactivity was sequenced and genecompared.

方法以hFGF7单克隆抗体为靶，进行4轮生物淘选噬菌体随机十二肽库；随机挑取单克隆噬菌体，应用ELISA方法检测单克隆噬菌体的结合力，对结合力较好的单克隆噬菌体提取噬菌体DNA，进行测序，并行序列分析。

Results The titer of the antiserum obtained in this experiment determined by ELISA was up to 1.16 000 and the sensitivity was at least 10 μg/L.

结果 此次获得的抗血清ELISA方法检测抗体滴度达到了1：16000，检测cTnI的灵敏度最低为10μ/L。

The titer and specificity of murine antiserum was evaluated by ELISA, Western blot and immunofluorescent staining.

用ELISA、Western blot和免疫荧光分别检测抗血清的效价和特异性。

After positive clones were induced by L arabinose for 4 hours, the purity of protein was detected by SDS PAGE and its bioactivity was identified by competitive inhibition ELISA test.

阳性克隆用左旋阿拉伯糖诱导4 h， SDS PAGE电泳检测蛋白纯度，竞争抑制ELISA实验检测其活性。

Objective To establish an ELISA for detecting specific antibody against Aspergillus fumigatus, and to evaluate its usefulness in diagnosis of invasive aspergillosis.

目的 建立检测人血清中抗烟曲霉特异性抗体的ELISA法，评估该法在侵袭性烟曲霉病诊断中的价值。

ELISA demonstrated that tetravalent antibody has gained in avidity compared with scFv and monoclonal antibody.

ELISA检测结果表明，四价抗体与单链抗体和单克隆抗体相比，具有更高的亲和力。

To evaluate the specificity of the PCR, genomic DNA of Theileria annulata,Babesia bovis,Toxoplasma gondii,Leishmania donovani and standard strain of N. caninum were used as a template in the PCR. For determining the detection limit of amplification procedure, PCR was run on a dilution series of genomic DNA from N. caninum（1.562 5-200 ng/ml）. Brain tissue samples of 32 aborted fetuses were detected by PCR-based assay, and 23 blood samples from mothers were tested by ELISA. Results The amplified DNA fragment （350 bp）had a high identity of 98% with the Nc-5 gene sequence of N.

以环形泰勒虫、牛巴贝斯虫、刚地弓形虫、杜氏利什曼原虫以及犬新孢子虫标准株DNA为模板进行扩增以验证PCR的特异性，采用紫外分光光度计测定犬新孢子虫标准株DNA浓度和纯度，取高纯度的DNA样品用灭菌水稀释，分别取不同量的DNA进行PCR扩增，确定PCR方法的敏感性；利用该方法对32份奶牛流产的胎牛脑组织样品进行检测，同时，对其中23份流产的母牛血样进行ELISA血清学检测，以评价犬新孢子虫PCR方法的检测效果。

Methods The serum levels of BAFF in rheumatic disease patients and normal controls were determined by ELISA.

方法采用ELISA方法对各种风湿病患者及健康对照者血中BAFF进行测定。

Peripheral blood taken from 58 cases of patients with autoimmune diseases and 20 healthy controls were collected, and the expression levels of APRIL as well as BAFF mRNA in these peripheral blood and the coresponding proteins expressed in the blood plasma were determined by real-time quantitative assay and ELISA respectively.

收集58名自身免疫病患者以及20名健康对照的外周血，用实时定量RT-PCR方法检测PBMC中APRIL和BAFF mRNA的表达，用ELISA方法检测血浆中APRIL和BAFF蛋白水平。

Objective To evaluate the detected difference of tumor markers between protein biochip and ELISA method.

目的 研究蛋白质芯片与ELISA法对肿瘤标志物检测结果的差异。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新