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The model of ECV-304 cell oxidative stress injury was established by hydrogen peroxide (H2O2).Then EPZ-contained blood serum was taken as experimental drug. The adherence of monocytes to endothelial cell were measured by method of rose Bengal. The total RNA of cells was extracted. The intercellular adhesion molecule-1 (ICAM-1),vascular cell adhesion molecule-1 (VCAM-1) and MCP-1 mRNA expression in cells were detected by RT-PCR.MCP-1 protein expression were detected by ELISA.

采用过氧化氢建立离体培养的血管内皮细胞(ECV-304)氧化应激损伤模型，取健康大鼠每日灌服不同剂量的鲜姜有效部位(200，400，800 mg·kg-1)或阳性对照药洛伐他汀(40 mg·kg-1)，取含药血清作为受试药物，用孟加拉玫红染色法测定单核细胞与内皮细胞的黏附力；逆转录聚合酶链反应测定细胞内细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子(VCAM-1)以及单核细胞趋化蛋白(MCP-1)的mRNA表达水平；酶联免疫吸附法测定MCP-1蛋白表达水平。

The results associated with clinical data were calculated statistically. Results: The androgen-independent human CaP cell line PC_3 produced relativelyhigh levels of secreted OPG protein in vitro, whereas the androgen-responsive cellline LNCaP produced low levels of OPG protein.

采用ELISA法测定血清OPG浓度，比较骨转移组与其它各组OPG浓度差异，结合收集前列腺癌患者临床资料分析与OPG浓度相关性，制作ROC曲线比较OPG与PSA、ALP对前列腺癌骨转移的诊断价值。

Polyclonal antibodies for Ochratoxin A were generated from rabbits after the animals had been immunized with either OTA -γ- globulin or OTA - keyhole limpet hemocyanin. A competitive indirect enzyme - linked immunosorbent assay and a competitive direct ELISA were used for the characterization of the antibodies and for analysis of OTA in coffee, corn, barley and bean samples.

本实验利用OTA -γ- globulin和OTA - KLH两种不同的OTA接合物为抗原来免疫兔子，进而收集免疫血清以获得抗OTA 的多株抗体，接著利用此一抗体来建立非直接型竞争酵素连结免疫吸附分析法和直接竞争型酵素连结免疫吸附分析法，并用cdELISA的方法加以侦测咖啡及玉米类、麦类、豆类样品中赭麴毒素A 的含量。

Cancer were analyzed by chemiluminescence immunoassay and enzyme linked lmmuno??sorbant assay, which control by 28 healthy.

方法采用化学发光和ELISA法测定28例胃癌、32例癌前疾病患者和28例对照者血清及胃液中CEA、CA199、CA242的含量。

Aprotinin was not added to the control group. MTT assay at 492nm was used to evaluate the erect of aprotinin on cell proliferation.

以四甲基偶氮唑盐法检测不同质量浓度抑肽酶对细胞存活率的影响；应用ELISA法检测用不同浓度的抑肽酶作用24h后上清液中TNF-α的含量。

MethodsThe levels of CEA,CA199 and CA242 in serum and gastric fluid that gathered from 28 cases of gastric cancer and 32 cases of precancer were analyzed by chemiluminescence immunoassay and enzyme linked lmmunosorbant assay, which control by 28 healthy.

方法采用化学发光和ELISA法测定28例胃癌、32例癌前疾病患者和28例对照者血清及胃液中CEA、CA199、CA242的含量。

Methods 72 SD rats were divided into experimental group and control group randomly, treated the experimental group with tretinoin and the control group with distilled water of equal quantity by intragastic administation for lasted 14 days, assayed the bone density with dual energy X-ray absorptiometry and serum hepcidin concentration of experimental group and control group with enzyme-linked immunosorbent assay.

取72只大鼠分为模型组和对照组，模型组以维甲酸70mg/持续灌胃14天，对照组等量蒸馏水灌胃14天，双能X线骨密度仪器进行活体骨密度测定，模型组和对照组血清hepcidin含量ELISA试剂盒进行测定。

Methods:Expression of MMP-9 was detected in the culture fluid of mononuclear cells from 30 patients with MM and MMP-2 in the culture fluid of bone marrow stromal cells from 24 patients with MM by ELISA.

用酶联免疫吸附试验检测了30例MM患者单个核细胞培养液的MMP-9水平、24例MM患者骨髓基质细胞培养液的MMP-2水平和12例对照组MMP-9、MMP-2水平。

After the stimulation of LPS, the expression of CXCR4 and the concentrations of TNF-α and IL-6 in culture fluid were detected through the methods of Western Blot, immunohistochemistry and RT-PCR respectively.

在LPS刺激肺泡巨噬细胞后，分别应用ELISA、Western Blot及RT-PCR法检测肺泡巨噬细胞培养液中TNF-a和IL-6浓度以及肺泡巨噬细胞CXCR4蛋白和mRNA表达的变化。

Methods Intrathymic injection of 2.5×107 Wistar spleen cells in neonatal SD rats was performed followed by mixing lymphocyte culture with Wistar rat spleen cells (20Gy) at 4-6 weeks of age. The levels of IL-4 and IFN-γ were measured by ELISA on the third day.

将2.5×107个Wistar大鼠脾淋巴细胞注入新生SD大鼠胸腺内，4～6周龄时取其脾淋巴细胞与Wistar大鼠脾淋巴细胞(20Gy X射线照射)进行混合培养，于72h测定IL-4和γ干扰素的含量。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新